FENR_STAA3
ID FENR_STAA3 Reviewed; 344 AA.
AC Q2FEC4;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Ferredoxin--NADP reductase {ECO:0000255|HAMAP-Rule:MF_01685};
DE Short=FNR {ECO:0000255|HAMAP-Rule:MF_01685};
DE Short=Fd-NADP(+) reductase {ECO:0000255|HAMAP-Rule:MF_01685};
DE EC=1.18.1.2 {ECO:0000255|HAMAP-Rule:MF_01685};
GN OrderedLocusNames=SAUSA300_2319;
OS Staphylococcus aureus (strain USA300).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=367830;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=USA300;
RX PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA Perdreau-Remington F.;
RT "Complete genome sequence of USA300, an epidemic clone of community-
RT acquired meticillin-resistant Staphylococcus aureus.";
RL Lancet 367:731-739(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2
CC oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:20125, Rhea:RHEA-
CC COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.18.1.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01685};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01685};
CC Note=Binds 1 FAD per subunit. {ECO:0000255|HAMAP-Rule:MF_01685};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01685}.
CC -!- SIMILARITY: Belongs to the ferredoxin--NADP reductase type 2 family.
CC {ECO:0000255|HAMAP-Rule:MF_01685}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000255; ABD21289.1; -; Genomic_DNA.
DR RefSeq; WP_000655971.1; NZ_CP027476.1.
DR PDB; 5TWB; X-ray; 1.82 A; A=1-344.
DR PDB; 5TWC; X-ray; 2.31 A; A=1-344.
DR PDBsum; 5TWB; -.
DR PDBsum; 5TWC; -.
DR AlphaFoldDB; Q2FEC4; -.
DR SMR; Q2FEC4; -.
DR EnsemblBacteria; ABD21289; ABD21289; SAUSA300_2319.
DR KEGG; saa:SAUSA300_2319; -.
DR HOGENOM; CLU_031864_5_5_9; -.
DR OMA; LEQCAPF; -.
DR Proteomes; UP000001939; Chromosome.
DR GO; GO:0004324; F:ferredoxin-NADP+ reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_01685; FENR2; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR022890; Fd--NADP_Rdtase_type_2.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW 3D-structure; FAD; Flavoprotein; NADP; Oxidoreductase.
FT CHAIN 1..344
FT /note="Ferredoxin--NADP reductase"
FT /id="PRO_0000364947"
FT BINDING 12
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 31
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 39
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 43
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 83
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 118
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 285
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT BINDING 326
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01685"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 11..22
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 32..36
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 38..42
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 59..70
FT /evidence="ECO:0007829|PDB:5TWB"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 76..78
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 83..90
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 93..98
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 103..111
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 117..120
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:5TWC"
FT HELIX 131..133
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 150..154
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 158..168
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 171..178
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 180..182
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 186..194
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 198..202
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 204..211
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 215..225
FT /evidence="ECO:0007829|PDB:5TWB"
FT TURN 226..228
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 231..235
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 237..241
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 245..248
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 251..254
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 255..257
FT /evidence="ECO:0007829|PDB:5TWC"
FT STRAND 261..265
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 270..274
FT /evidence="ECO:0007829|PDB:5TWB"
FT STRAND 280..282
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 296..314
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 325..327
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 329..331
FT /evidence="ECO:0007829|PDB:5TWB"
FT HELIX 332..342
FT /evidence="ECO:0007829|PDB:5TWB"
SQ SEQUENCE 344 AA; 38230 MW; 46E1A319359751D3 CRC64;
MKDVTIIGGG PSGLYASFYA GLRDMSVRLI DVQSELGGKM RIYPEKIIWD IGGIAPKPCH
EILKDTIKQG LYFKPEVHLN ERVVDIRKKA ERHFEVETEA GEIYTSKAVI IAIGAGIINP
KQLDVKGVER YQLTNLHYVV QSYRRFKDKD VLISGGGNTA LDWAHDIAKI AKSVTVVYRK
EDVSGHEAMK TLVTDLNVKL CPKTRIKYLV GNDDETHISE VVLEHVESGD RHTVKFDDVI
ISHGFDRCNT LLSETSSKLD MHDDCRVKGF GNTTTSIPGI YACGDIVYHD AKSHLIASAF
SDGANAANLA KTYIQPDANA EGYVSSHHEV FKEANKTIVN KHLY
