FEPD_ECOLI
ID FEPD_ECOLI Reviewed; 334 AA.
AC P23876; P77097; Q2MBL1;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Ferric enterobactin transport system permease protein FepD {ECO:0000305};
GN Name=fepD; OrderedLocusNames=b0590, JW0582;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=1838574; DOI=10.1111/j.1365-2958.1991.tb00788.x;
RA Shea C.M., McIntosh M.A.;
RT "Nucleotide sequence and genetic organization of the ferric enterobactin
RT transport system: homology to other periplasmic binding protein-dependent
RT systems in Escherichia coli.";
RL Mol. Microbiol. 5:1415-1428(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1787794; DOI=10.1111/j.1365-2958.1991.tb00787.x;
RA Chenault S.S., Earhart C.F.;
RT "Organization of genes encoding membrane proteins of the Escherichia coli
RT ferrienterobactin permease.";
RL Mol. Microbiol. 5:1405-1413(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=1479347; DOI=10.1099/00221287-138-10-2167;
RA Chenault S.S., Earhart C.F.;
RT "Identification of hydrophobic proteins FepD and FepG of the Escherichia
RT coli ferrienterobactin permease.";
RL J. Gen. Microbiol. 138:2167-2171(1992).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [8]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Part of the ABC transporter complex FepBDGC involved in
CC ferric enterobactin uptake (PubMed:1838574, PubMed:1479347).
CC Responsible for the translocation of the substrate across the membrane
CC (Probable). {ECO:0000269|PubMed:1479347, ECO:0000269|PubMed:1838574,
CC ECO:0000305}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (FepC),
CC two transmembrane proteins (FepD and FepG) and a solute-binding protein
CC (FepB). {ECO:0000305|PubMed:1479347}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:1479347,
CC ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Controlled in part by the amount of available iron
CC (PubMed:1838574). Induced by hydroxyurea (PubMed:20005847).
CC {ECO:0000269|PubMed:1838574, ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. FecCD subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40789.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X57471; CAA40707.1; -; Genomic_DNA.
DR EMBL; X59402; CAA42043.1; -; Genomic_DNA.
DR EMBL; U82598; AAB40789.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73691.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76345.1; -; Genomic_DNA.
DR PIR; S16296; S16296.
DR RefSeq; NP_415122.1; NC_000913.3.
DR RefSeq; WP_000016947.1; NZ_SSZK01000032.1.
DR AlphaFoldDB; P23876; -.
DR SMR; P23876; -.
DR BioGRID; 4261558; 236.
DR ComplexPortal; CPX-4404; Ferric-enterobactin ABC transporter complex.
DR STRING; 511145.b0590; -.
DR TCDB; 3.A.1.14.2; the atp-binding cassette (abc) superfamily.
DR PaxDb; P23876; -.
DR PRIDE; P23876; -.
DR EnsemblBacteria; AAC73691; AAC73691; b0590.
DR EnsemblBacteria; BAE76345; BAE76345; BAE76345.
DR GeneID; 945214; -.
DR KEGG; ecj:JW0582; -.
DR KEGG; eco:b0590; -.
DR PATRIC; fig|1411691.4.peg.1679; -.
DR EchoBASE; EB0292; -.
DR eggNOG; COG0609; Bacteria.
DR HOGENOM; CLU_013016_1_0_6; -.
DR InParanoid; P23876; -.
DR OMA; VFIWIVR; -.
DR PhylomeDB; P23876; -.
DR BioCyc; EcoCyc:FEPD-MON; -.
DR BioCyc; MetaCyc:FEPD-MON; -.
DR PRO; PR:P23876; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015620; F:ferric-enterobactin transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015685; P:ferric-enterobactin import into cell; IMP:EcoCyc.
DR GO; GO:0033212; P:iron import into cell; IC:ComplexPortal.
DR GO; GO:0033214; P:siderophore-dependent iron import into cell; IBA:GO_Central.
DR Gene3D; 1.10.3470.10; -; 1.
DR InterPro; IPR037294; ABC_BtuC-like.
DR InterPro; IPR000522; ABC_transptr_permease_BtuC.
DR PANTHER; PTHR30472; PTHR30472; 1.
DR Pfam; PF01032; FecCD; 1.
DR SUPFAM; SSF81345; SSF81345; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion transport; Iron; Iron transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..334
FT /note="Ferric enterobactin transport system permease
FT protein FepD"
FT /id="PRO_0000060022"
FT TOPO_DOM 1..9
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..63
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..92
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..151
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..192
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..241
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..305
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
FT CONFLICT 233..234
FT /note="RV -> AL (in Ref. 2; CAA42043)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 33871 MW; BB5C5E939A96951B CRC64;
MSGSVAVTRA IAVPGLLLLL IIATALSLLI GAKSLPASVV LEAFSGTCQS ADCTIVLDAR
LPRTLAGLLA GGALGLAGAL MQTLTRNPLA DPGLLGVNAG ASFAIVLGAA LFGYSSAQEQ
LAMAFAGALV ASLIVAFTGS QGGGQLSPVR LTLAGVALAA VLEGLTSGIA LLNPDVYDQL
RFWQAGSLDI RNLHTLKVVL IPVLIAGATA LLLSRALNSL SLGSDTATAL GSRVARTQLI
GLLAITVLCG SATAIVGPIA FIGLMMPHMA RWLVGADHRW SLPVTLLATP ALLLFADIIG
RVIVPGELRV SVVSAFIGAP VLIFLVRRKT RGGA
