FER1_SULTO
ID FER1_SULTO Reviewed; 104 AA.
AC P55907; O32423;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Zinc-containing ferredoxin-1;
GN Name=zfx1; Synonyms=zfx; OrderedLocusNames=STK_01630;
OS Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS (Sulfolobus tokodaii).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfurisphaera.
OX NCBI_TaxID=273063;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=8753789; DOI=10.1006/bbrc.1996.1200;
RA Wakagi T., Fujii T., Oshima T.;
RT "Molecular cloning, sequencing, and heterologous expression of a novel
RT zinc-containing ferredoxin gene from a thermoacidophilic Archaeon
RT Sulfolobus sp. strain 7.";
RL Biochem. Biophys. Res. Commun. 225:489-493(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT Sulfolobus tokodaii strain7.";
RL DNA Res. 8:123-140(2001).
RN [3]
RP PROTEIN SEQUENCE OF 2-31, AND METHYLATION AT LYS-30.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=7826373; DOI=10.1006/bbrc.1995.1080;
RA Iwasaki T., Fujii T., Wakagi T., Oshima T.;
RT "Alternative form of the dicluster ferredoxin from the thermoacidophilic
RT archaeon, Sulfolobus sp. strain 7.";
RL Biochem. Biophys. Res. Commun. 206:563-569(1995).
RN [4]
RP CHARACTERIZATION.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=7961925; DOI=10.1016/s0021-9258(18)43899-9;
RA Iwasaki T., Wakagi T., Isogai Y., Tanaka K., Iizuka T., Oshima T.;
RT "Functional and evolutionary implications of a [3Fe-4S] cluster of the
RT dicluster-type ferredoxin from the thermoacidophilic archaeon, Sulfolobus
RT sp. strain 7.";
RL J. Biol. Chem. 269:29444-29450(1994).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=9063899; DOI=10.1021/bi961966j;
RA Fujii T., Hata Y., Oozeki M., Moriyama H., Wakagi T., Tanaka N., Oshima T.;
RT "The crystal structure of zinc-containing ferredoxin from the
RT thermoacidophilic archaeon Sulfolobus sp. strain 7.";
RL Biochemistry 36:1505-1513(1997).
CC -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons
CC in a wide variety of metabolic reactions.
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Note=Binds 1 [3Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Note=Binds 1 [4Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Note=Binds 1 zinc ion.;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Redox potential:
CC E(0) is -280 mV for the 3Fe-4S and -530 mV for the 4Fe-4S clusters.;
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DR EMBL; D78179; BAA22587.1; -; Genomic_DNA.
DR EMBL; BA000023; BAB65120.1; -; Genomic_DNA.
DR PIR; JC4907; JC4907.
DR RefSeq; WP_010978102.1; NC_003106.2.
DR PDB; 1XER; X-ray; 2.00 A; A=2-104.
DR PDBsum; 1XER; -.
DR AlphaFoldDB; P55907; -.
DR SMR; P55907; -.
DR STRING; 273063.STK_01630; -.
DR iPTMnet; P55907; -.
DR EnsemblBacteria; BAB65120; BAB65120; STK_01630.
DR GeneID; 54121801; -.
DR KEGG; sto:STK_01630; -.
DR PATRIC; fig|273063.9.peg.200; -.
DR eggNOG; arCOG04548; Archaea.
DR OMA; QFHENRD; -.
DR OrthoDB; 111853at2157; -.
DR EvolutionaryTrace; P55907; -.
DR Proteomes; UP000001015; Chromosome.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR009157; Fd_Zn-bd.
DR Pfam; PF13187; Fer4_9; 1.
DR PIRSF; PIRSF000068; Zn_Fdx_Sulfol; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; 3Fe-4S; 4Fe-4S; Direct protein sequencing;
KW Electron transport; Iron; Iron-sulfur; Metal-binding; Methylation;
KW Reference proteome; Repeat; Transport; Zinc.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7826373"
FT CHAIN 2..104
FT /note="Zinc-containing ferredoxin-1"
FT /id="PRO_0000159180"
FT DOMAIN 38..66
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 75..104
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT REGION 2..37
FT /note="N-terminal extension"
FT BINDING 17
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 20
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 46
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT BINDING 52
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT BINDING 56
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 77
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 84
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 87
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 90
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 94
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT MOD_RES 30
FT /note="N6-methyllysine"
FT /evidence="ECO:0000303|PubMed:7826373"
FT CONFLICT 26
FT /note="V -> L (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="N -> T (in Ref. 1; BAA22587)"
FT /evidence="ECO:0000305"
FT HELIX 7..10
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 13..17
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 20..24
FT /evidence="ECO:0007829|PDB:1XER"
FT TURN 28..30
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:1XER"
FT TURN 43..45
FT /evidence="ECO:0007829|PDB:1XER"
FT HELIX 51..55
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:1XER"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:1XER"
FT HELIX 89..93
FT /evidence="ECO:0007829|PDB:1XER"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:1XER"
SQ SEQUENCE 104 AA; 11134 MW; 04A9E52F5EDA4F4A CRC64;
MGIDPNYRTN RQVVGEHSGH KVYGPVEPPK VLGIHGTIVG VDFDLCIADG SCINACPVNV
FQWYDTPGHP ASEKKADPVN EQACIFCMAC VNVCPVAAID VKPP
