FER2_AZOVD
ID FER2_AZOVD Reviewed; 107 AA.
AC P82802; C1DH09;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Ferredoxin, 2Fe-2s;
DE AltName: Full=2FeAvFdI;
DE AltName: Full=Iron-sulfur protein I;
DE AltName: Full=Shethna protein I;
GN OrderedLocusNames=Avin_01520;
OS Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=322710;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DJ / ATCC BAA-1303;
RX PubMed=19429624; DOI=10.1128/jb.00504-09;
RA Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A.,
RA Houmiel K., Imperial J., Kennedy C., Larson T.J., Latreille P., Ligon L.S.,
RA Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I.,
RA Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L.,
RA Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H.,
RA Dean D.R., Dixon R., Wood D.;
RT "Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized
RT to support diverse anaerobic metabolic processes.";
RL J. Bacteriol. 191:4534-4545(2009).
RN [2]
RP PROTEIN SEQUENCE OF 2-107, SUBUNIT, AND MASS SPECTROMETRY.
RX PubMed=10439076; DOI=10.1007/s007750050317;
RA Chatelet C., Meyer J.;
RT "The [2Fe-2S] protein I (Shethna protein I) from Azotobacter vinelandii is
RT homologous to the [2Fe-2S] ferredoxin from Clostridium pasteurianum.";
RL J. Biol. Inorg. Chem. 4:311-317(1999).
CC -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons
CC in a wide variety of metabolic reactions. Might be involved in nitrogen
CC fixation.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Note=Binds 1 [2Fe-2S] cluster.;
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10439076}.
CC -!- MASS SPECTROMETRY: Mass=22876; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10439076};
CC -!- SIMILARITY: Belongs to the 2Fe2S Shethna-type ferredoxin family.
CC {ECO:0000305}.
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DR EMBL; CP001157; ACO76416.1; -; Genomic_DNA.
DR RefSeq; WP_012698844.1; NC_012560.1.
DR PDB; 5ABR; X-ray; 2.11 A; A/B=1-107.
DR PDBsum; 5ABR; -.
DR AlphaFoldDB; P82802; -.
DR SMR; P82802; -.
DR STRING; 322710.Avin_01520; -.
DR PRIDE; P82802; -.
DR EnsemblBacteria; ACO76416; ACO76416; Avin_01520.
DR KEGG; avn:Avin_01520; -.
DR eggNOG; COG3411; Bacteria.
DR HOGENOM; CLU_126515_1_1_6; -.
DR OMA; GICHKKE; -.
DR OrthoDB; 1412344at2; -.
DR Proteomes; UP000002424; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Direct protein sequencing; Electron transport; Iron;
KW Iron-sulfur; Metal-binding; Nitrogen fixation; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:10439076"
FT CHAIN 2..107
FT /note="Ferredoxin, 2Fe-2s"
FT /id="PRO_0000187247"
FT BINDING 11
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT BINDING 24
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT BINDING 56
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT BINDING 60
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:5ABR"
FT TURN 24..28
FT /evidence="ECO:0007829|PDB:5ABR"
FT HELIX 29..42
FT /evidence="ECO:0007829|PDB:5ABR"
FT TURN 46..48
FT /evidence="ECO:0007829|PDB:5ABR"
FT STRAND 49..55
FT /evidence="ECO:0007829|PDB:5ABR"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:5ABR"
FT STRAND 66..69
FT /evidence="ECO:0007829|PDB:5ABR"
FT TURN 70..72
FT /evidence="ECO:0007829|PDB:5ABR"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:5ABR"
FT HELIX 82..89
FT /evidence="ECO:0007829|PDB:5ABR"
FT TURN 90..93
FT /evidence="ECO:0007829|PDB:5ABR"
FT HELIX 98..100
FT /evidence="ECO:0007829|PDB:5ABR"
FT TURN 104..106
FT /evidence="ECO:0007829|PDB:5ABR"
SQ SEQUENCE 107 AA; 11395 MW; 44A9DB807959F794 CRC64;
MAKPEFHIFI CAQNRPAGHP RGSCGAKGAE GVYNAFAQVL IQKNLTNRIA LTTTGCLGPC
QAGANVLIYP GAVMYSWVEP ADAAIIVEQH LLGGEPYADK LTPAEIW