FER3_DROME
ID FER3_DROME Reviewed; 195 AA.
AC Q9VGJ5; A0AQE5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein Fer3;
DE AltName: Full=Basic helix-loop-helix protein N-twist;
DE AltName: Full=Nephew of atonal 3;
DE AltName: Full=Neuronal twist;
GN Name=fer3; Synonyms=Nato3, Ntwist; ORFNames=CG6913;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=11472856; DOI=10.1016/s0925-4773(01)00437-3;
RA Segev E., Halachmi N., Salzberg A., Ben-Arie N.;
RT "Nato3 is an evolutionarily conserved bHLH transcription factor expressed
RT in the CNS of Drosophila and mouse.";
RL Mech. Dev. 106:197-202(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=12217327; DOI=10.1006/dbio.2002.0753;
RA Verzi M.P., Anderson J.P., Dodou E., Kelly K.K., Greene S.B., North B.J.,
RA Cripps R.M., Black B.L.;
RT "N-twist, an evolutionarily conserved bHLH protein expressed in the
RT developing CNS, functions as a transcriptional inhibitor.";
RL Dev. Biol. 249:174-190(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16951084; DOI=10.1534/genetics.106.058008;
RA Proeschel M., Zhang Z., Parsch J.;
RT "Widespread adaptive evolution of Drosophila genes with sex-biased
RT expression.";
RL Genetics 174:893-900(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP IDENTIFICATION, AND DEVELOPMENTAL STAGE.
RX PubMed=10973473; DOI=10.1073/pnas.170301897;
RA Moore A.W., Barbel S., Jan L.Y., Jan Y.N.;
RT "A genomewide survey of basic helix-loop-helix factors in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10436-10441(2000).
CC -!- FUNCTION: Transcription factor that binds to the E-box and functions as
CC inhibitor of transcription. DNA binding requires dimerization with an E
CC protein. Inhibits transcription activation by ASCL1/MASH1 by
CC sequestering E proteins (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DEVELOPMENTAL STAGE: First detected in 6 to 9 hour old embryos.
CC Expression is highest in 9 to 15 hour old embryos. First detected in
CC stage 11 embryos within the neurogenic region. Detected in the
CC procephalic region, the ventral nerve cord, subesophagal, thoracic and
CC abdominal ganglia in stage 13 to 14 embryos. Detected in embryonic
CC midgut primordia. Barely detectable at the first stage of larval
CC development. Not detectable in older larvae, pupae or adults.
CC {ECO:0000269|PubMed:10973473, ECO:0000269|PubMed:11472856,
CC ECO:0000269|PubMed:12217327}.
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DR EMBL; AF369898; AAK72957.1; -; Genomic_DNA.
DR EMBL; AF517123; AAN04087.1; -; mRNA.
DR EMBL; AM294883; CAL26869.1; -; Genomic_DNA.
DR EMBL; AM294884; CAL26870.1; -; Genomic_DNA.
DR EMBL; AM294885; CAL26871.1; -; Genomic_DNA.
DR EMBL; AM294886; CAL26872.1; -; Genomic_DNA.
DR EMBL; AM294887; CAL26873.1; -; Genomic_DNA.
DR EMBL; AM294888; CAL26874.1; -; Genomic_DNA.
DR EMBL; AM294889; CAL26875.1; -; Genomic_DNA.
DR EMBL; AM294890; CAL26876.1; -; Genomic_DNA.
DR EMBL; AM294891; CAL26877.1; -; Genomic_DNA.
DR EMBL; AM294892; CAL26878.1; -; Genomic_DNA.
DR EMBL; AM294893; CAL26879.1; -; Genomic_DNA.
DR EMBL; AM294894; CAL26880.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54684.2; -; Genomic_DNA.
DR EMBL; AY094777; AAM11130.1; -; mRNA.
DR RefSeq; NP_524322.1; NM_079598.3.
DR AlphaFoldDB; Q9VGJ5; -.
DR SMR; Q9VGJ5; -.
DR BioGRID; 66537; 7.
DR IntAct; Q9VGJ5; 5.
DR STRING; 7227.FBpp0081911; -.
DR PaxDb; Q9VGJ5; -.
DR DNASU; 41411; -.
DR EnsemblMetazoa; FBtr0082435; FBpp0081911; FBgn0037937.
DR GeneID; 41411; -.
DR KEGG; dme:Dmel_CG6913; -.
DR CTD; 41411; -.
DR FlyBase; FBgn0037937; Fer3.
DR VEuPathDB; VectorBase:FBgn0037937; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00990000206751; -.
DR HOGENOM; CLU_099192_0_0_1; -.
DR InParanoid; Q9VGJ5; -.
DR OMA; RDFASPY; -.
DR OrthoDB; 1513995at2759; -.
DR PhylomeDB; Q9VGJ5; -.
DR BioGRID-ORCS; 41411; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 41411; -.
DR PRO; PR:Q9VGJ5; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037937; Expressed in presumptive embryonic/larval central nervous system and 6 other tissues.
DR ExpressionAtlas; Q9VGJ5; baseline and differential.
DR Genevisible; Q9VGJ5; DM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:FlyBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..195
FT /note="Protein Fer3"
FT /id="PRO_0000328681"
FT DOMAIN 86..138
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 56..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 7
FT /note="I -> S (in Ref. 3; CAL26880/CAL26879/CAL26878/
FT CAL26877/CAL26876/CAL26875/CAL26874/CAL26873/CAL26872/
FT CAL26871/CAL26870/CAL26869)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 22124 MW; A3AC33803B9D71B5 CRC64;
MQHPHPIDQP TYMPDVPFQP LWGQEAPPPP IVPYQELIAG FPCTDLSLWQ RSQVTPLVPQ
RPSTNGRANG SSSSSKKTRR RVASMAQRRA ANIRERRRMF NLNEAFDKLR RKVPTFAYEK
RLSRIETLRL AITYIGFMAE LLSGTPSNSH KSRSDVYGSM NGHHQAPPPA IHPHHLHPAA
AYQRDFASPY NHSLS
