FER_HYDSH
ID FER_HYDSH Reviewed; 78 AA.
AC Q45560;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Ferredoxin 7Fe;
DE AltName: Full=Seven-iron ferredoxin;
GN Name=fdxA;
OS Hydrogenibacillus schlegelii (Bacillus schlegelii).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Hydrogenibacillus.
OX NCBI_TaxID=1484;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43741 / DSM 2000 / CCUG 26017 / CIP 106933 / NCIMB 13107 /
RC MA-48;
RX PubMed=8003034; DOI=10.1006/bbrc.1994.1792;
RA Aono S., Nakamura S., Aono R., Okura I.;
RT "Cloning and expression of the gene encoding the 7Fe type ferredoxin from a
RT thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii.";
RL Biochem. Biophys. Res. Commun. 201:938-942(1994).
RN [2]
RP CHARACTERIZATION.
RX PubMed=1338329; DOI=10.1093/oxfordjournals.jbchem.a123977;
RA Aono S., Kurita H., Uno S., Okura I.;
RT "Purification and characterization of a 7Fe ferredoxin from a thermophilic
RT hydrogen-oxidizing bacterium, Bacillus schlegelii.";
RL J. Biochem. 112:792-795(1992).
RN [3]
RP STRUCTURE BY NMR.
RC STRAIN=ATCC 43741 / DSM 2000 / CCUG 26017 / CIP 106933 / NCIMB 13107 /
RC MA-48;
RX PubMed=9657695; DOI=10.1021/bi972818b;
RA Aono S., Bentrop D., Bertini I., Donaire A., Luchinat C., Niikura Y.,
RA Rosato A.;
RT "Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic
RT bacterium Bacillus schlegelii by 1H NMR spectroscopy.";
RL Biochemistry 37:9812-9826(1998).
RN [4]
RP MUTAGENESIS OF ASP-14.
RX PubMed=9276454; DOI=10.1016/s0014-5793(97)00834-x;
RA Aono S., Bentrop D., Bertini I., Luchinat C., Macinai R.;
RT "The D13C variant of Bacillus schlegelii 7Fe ferredoxin is an 8Fe
RT ferredoxin as revealed by 1H-NMR spectroscopy.";
RL FEBS Lett. 412:501-505(1997).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Note=Binds 1 [4Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Note=Binds 1 [3Fe-4S] cluster.;
CC -!- SUBUNIT: Monomer.
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DR EMBL; D29804; BAA06187.1; -; Genomic_DNA.
DR PIR; JC2496; JC2496.
DR RefSeq; WP_066198812.1; NZ_JYFD01000154.1.
DR PDB; 1BC6; NMR; -; A=2-78.
DR PDB; 1BD6; NMR; -; A=2-78.
DR PDB; 1BQX; NMR; -; A=2-78.
DR PDB; 1BWE; NMR; -; A=2-78.
DR PDBsum; 1BC6; -.
DR PDBsum; 1BD6; -.
DR PDBsum; 1BQX; -.
DR PDBsum; 1BWE; -.
DR AlphaFoldDB; Q45560; -.
DR BMRB; Q45560; -.
DR SMR; Q45560; -.
DR STRING; 1484.SA87_06055; -.
DR EvolutionaryTrace; Q45560; -.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR000813; 7Fe_ferredoxin.
DR Pfam; PF00037; Fer4; 1.
DR PRINTS; PR00354; 7FE8SFRDOXIN.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; 3Fe-4S; 4Fe-4S; Electron transport; Iron; Iron-sulfur;
KW Metal-binding; Repeat; Transport.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..78
FT /note="Ferredoxin 7Fe"
FT /id="PRO_0000159172"
FT DOMAIN 2..29
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 31..60
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 9
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT BINDING 17
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT BINDING 21
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 40
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 43
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 46
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 50
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT MUTAGEN 14
FT /note="D->C: Transformation from a 7S to 8S ferredoxin."
FT /evidence="ECO:0000269|PubMed:9276454"
FT TURN 8..11
FT /evidence="ECO:0007829|PDB:1BC6"
FT TURN 18..20
FT /evidence="ECO:0007829|PDB:1BC6"
FT STRAND 26..28
FT /evidence="ECO:0007829|PDB:1BC6"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:1BC6"
FT TURN 37..39
FT /evidence="ECO:0007829|PDB:1BC6"
FT HELIX 46..49
FT /evidence="ECO:0007829|PDB:1BC6"
FT HELIX 51..53
FT /evidence="ECO:0007829|PDB:1BC6"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:1BC6"
FT TURN 58..60
FT /evidence="ECO:0007829|PDB:1BC6"
FT HELIX 63..75
FT /evidence="ECO:0007829|PDB:1BC6"
SQ SEQUENCE 78 AA; 8874 MW; 3295879B2E8C2BAA CRC64;
MAYVITEPCI GTKDASCVEV CPVDCIHEGE DQYYIDPDVC IDCGACEAVC PVSAIYHEDF
VPEEWKSYIQ KNRDFFKK
