FER_PYRFU
ID FER_PYRFU Reviewed; 67 AA.
AC P29603;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Ferredoxin;
GN Name=fdxA; OrderedLocusNames=PF1909;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=8045914; DOI=10.1128/jb.176.15.4790-4793.1994;
RA Heltzel A., Smith E.T., Zhou Z.H., Blamey J.M., Adams M.W.W.;
RT "Cloning, expression, and molecular characterization of the gene encoding
RT an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic
RT archaeon Pyrococcus furiosus.";
RL J. Bacteriol. 176:4790-4793(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [3]
RP MUTAGENESIS OF ASP-15, CIRCULAR DICHROISM ANALYSIS, AND EPR SPECTROSCOPY.
RX PubMed=9283079; DOI=10.1021/bi9708141;
RA Zhou Z.H., Adams M.W.W.;
RT "Site-directed mutations of the 4Fe-ferredoxin from the hyperthermophilic
RT archaeon Pyrococcus furiosus: role of the cluster-coordinating aspartate in
RT physiological electron transfer reactions.";
RL Biochemistry 36:10892-10900(1997).
RN [4]
RP MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, AND
RP RESONANCE RAMAN SPECTROSCOPY OF (3FE--4S) FORM.
RA Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.;
RT "Spectroscopic evidence for a new type of [Fe3S4] cluster in a mutant form
RT of Pyrococcus furiosus ferredoxin.";
RL J. Am. Chem. Soc. 120:8525-8526(1998).
RN [5]
RP MUTAGENESIS OF CYSTEINE RESIDUES, MAGNETIC CIRCULAR DICHROISM, EPR
RP SPECTROSCOPY, AND RESONANCE RAMAN SPECTROSCOPY.
RX PubMed=10441157; DOI=10.1021/bi990671d;
RA Brereton P.S., Duderstadt R.E., Staples C.R., Johnson M.K., Adams M.W.W.;
RT "Effect of serinate ligation at each of the iron sites of the [Fe4S4]
RT cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and
RT biological properties.";
RL Biochemistry 38:10594-10605(1999).
RN [6]
RP MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, AND
RP RESONANCE RAMAN SPECTROSCOPY.
RX PubMed=10413088; DOI=10.1016/s0014-5793(99)00766-8;
RA Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.;
RT "A pure S=3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus
RT ferredoxin.";
RL FEBS Lett. 454:21-26(1999).
RN [7]
RP STRUCTURE BY NMR.
RX PubMed=1445925; DOI=10.1021/bi00162a038;
RA Busse S.C., la Mar G.N., Yu L.P., Howard J.B., Smith E.T., Zhou Z.H.,
RA Adams M.W.W.;
RT "Proton NMR investigation of the oxidized three-iron clusters in the
RT ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and
RT Thermococcus litoralis.";
RL Biochemistry 31:11952-11962(1992).
RN [8]
RP STRUCTURE BY NMR OF 3FE--4S FORM.
RX PubMed=8193147; DOI=10.1021/bi00186a035;
RA Teng Q., Zhou Z.H., Smith E.T., Busse S.C., Howard J.B., Adams M.W.W.,
RA La Mar G.N.;
RT "Solution 1H NMR determination of secondary structure for the three-iron
RT form of ferredoxin from the hyperthermophilic archaeon Pyrococcus
RT furiosus.";
RL Biochemistry 33:6316-6326(1994).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=7547865; DOI=10.1021/bi00036a010;
RA Calzolai L., Gorst C.M., Zhou Z.H., Teng Q., Adams M.W.W., La Mar G.N.;
RT "1H NMR investigation of the electronic and molecular structure of the
RT four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus.
RT Identification of Asp 14 as a cluster ligand in each of the four redox
RT states.";
RL Biochemistry 34:11373-11384(1995).
RN [10]
RP STRUCTURE BY NMR OF 3FE--4S FORM.
RX PubMed=7819255; DOI=10.1021/bi00002a027;
RA Gorst C.M., Yeh Y.-H., Teng Q., Calzolai L., Zhou Z.H., Adams M.W.W.,
RA La Mar G.N.;
RT "1H NMR investigation of the paramagnetic cluster environment in Pyrococcus
RT furiosus three-iron ferredoxin: sequence-specific assignment of ligated
RT cysteines independent of tertiary structure.";
RL Biochemistry 34:600-610(1995).
RN [11]
RP STRUCTURE BY NMR, AND DISULFIDE BOND.
RX PubMed=7612619; DOI=10.1021/bi00027a030;
RA Gorst C.M., Zhou Z.H., Teng Q., Howard J.B., Adams M.W.W., La Mar G.N.;
RT "Participation of the disulfide bridge in the redox cycle of the ferredoxin
RT from the hyperthermophile Pyrococcus furiosus: 1H nuclear magnetic
RT resonance time resolution of the four redox states at ambient
RT temperature.";
RL Biochemistry 34:8788-8795(1995).
RN [12]
RP STRUCTURE BY NMR, AND MUTAGENESIS OF ASP-15.
RA Calzolai L., Zhou Z.H., Adams M.W.W., La Mar G.N.;
RT "Role of cluster-ligated aspartate in gating electron transfer in the four-
RT iron ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.";
RL J. Am. Chem. Soc. 118:2513-2514(1996).
RN [13]
RP STRUCTURE BY NMR, AND MUTAGENESIS OF ASP-15.
RA Calzolai L., Gorst C.M., Bren K.L., Zhou Z.H., Adams M.W.W., La Mar G.N.;
RT "Solution NMR study of the electronic structure and magnetic properties of
RT cluster ligation mutants of the four-iron ferredoxin from the
RT hyperthermophilic archaeon Pyrococcus furiosus.";
RL J. Am. Chem. Soc. 119:9341-9350(1997).
RN [14]
RP STRUCTURE BY NMR OF 3FE--4S FORM.
RX PubMed=11601981; DOI=10.1021/bi0106179;
RA Webba da Silva M., Sham S., Gorst C.M., Calzolai L., Brereton P.S.,
RA Adams M.W.W., La Mar G.N.;
RT "Solution NMR characterization of the thermodynamics of the disulfide bond
RT orientational isomerism and its effect of cluster electronic properties for
RT the hyperthermostable three-iron cluster ferredoxin from the archaeon
RT Pyrococcus furiosus.";
RL Biochemistry 40:12575-12583(2001).
RN [15]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTER,
RP AND SUBUNIT.
RX PubMed=15122884; DOI=10.1021/bi049942x;
RA Nielsen M.S., Harris P., Ooi B.L., Christensen H.E.;
RT "The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the
RT hyperthermophilic archaeon Pyrococcus furiosus.";
RL Biochemistry 43:5188-5194(2004).
CC -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons
CC in a wide variety of metabolic reactions.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Note=Binds 1 [4Fe-4S] cluster which readily converts to a stable [3Fe-
CC 4S] form.;
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
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DR EMBL; X79502; CAA56040.1; -; Genomic_DNA.
DR EMBL; AE009950; AAL82033.1; -; Genomic_DNA.
DR PIR; A44203; A44203.
DR RefSeq; WP_011013049.1; NC_018092.1.
DR PDB; 1SIZ; X-ray; 2.25 A; A/C=2-67.
DR PDB; 1SJ1; X-ray; 1.50 A; A/B=2-67.
DR PDB; 2Z8Q; X-ray; 1.70 A; A/B=2-67.
DR PDB; 3PNI; X-ray; 2.80 A; A/B=2-67.
DR PDB; 4DHV; X-ray; 1.95 A; A/B=2-67.
DR PDBsum; 1SIZ; -.
DR PDBsum; 1SJ1; -.
DR PDBsum; 2Z8Q; -.
DR PDBsum; 3PNI; -.
DR PDBsum; 4DHV; -.
DR AlphaFoldDB; P29603; -.
DR SMR; P29603; -.
DR STRING; 186497.PF1909; -.
DR EnsemblBacteria; AAL82033; AAL82033; PF1909.
DR GeneID; 41713730; -.
DR KEGG; pfu:PF1909; -.
DR PATRIC; fig|186497.12.peg.1980; -.
DR eggNOG; arCOG00349; Archaea.
DR HOGENOM; CLU_139698_6_4_2; -.
DR OMA; DVFEMND; -.
DR OrthoDB; 111853at2157; -.
DR PhylomeDB; P29603; -.
DR EvolutionaryTrace; P29603; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR InterPro; IPR001080; 3Fe4S_ferredoxin.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR PRINTS; PR00352; 3FE4SFRDOXIN.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; 3Fe-4S; 4Fe-4S; Disulfide bond; Electron transport; Iron;
KW Iron-sulfur; Metal-binding; Reference proteome; Repeat; Transport.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..67
FT /note="Ferredoxin"
FT /id="PRO_0000159199"
FT DOMAIN 3..31
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 36..67
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 12
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 15
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 18
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT BINDING 57
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT DISULFID 22..49
FT /evidence="ECO:0000269|PubMed:7612619,
FT ECO:0000269|PubMed:7819255"
FT MUTAGEN 15
FT /note="D->C: No effect on the efficiency of electron
FT transfer."
FT /evidence="ECO:0000269|PubMed:9283079, ECO:0000269|Ref.12,
FT ECO:0000269|Ref.13"
FT MUTAGEN 15
FT /note="D->S: The efficiency of electron transfer is
FT compromised."
FT /evidence="ECO:0000269|PubMed:9283079, ECO:0000269|Ref.12,
FT ECO:0000269|Ref.13"
FT MUTAGEN 34
FT /note="A->C: The mixed spin cluster is converted into a
FT homogeneous spin cluster (S=3/2)."
FT /evidence="ECO:0000269|PubMed:10413088, ECO:0000269|Ref.4"
FT STRAND 3..7
FT /evidence="ECO:0007829|PDB:1SJ1"
FT TURN 9..11
FT /evidence="ECO:0007829|PDB:1SJ1"
FT HELIX 17..21
FT /evidence="ECO:0007829|PDB:1SJ1"
FT TURN 23..25
FT /evidence="ECO:0007829|PDB:1SJ1"
FT STRAND 26..28
FT /evidence="ECO:0007829|PDB:1SJ1"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:1SJ1"
FT HELIX 44..56
FT /evidence="ECO:0007829|PDB:1SJ1"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:1SJ1"
SQ SEQUENCE 67 AA; 7298 MW; AF9B062690B34420 CRC64;
MAWKVSVDQD TCIGDAICAS LCPDVFEMND EGKAQPKVEV IEDEELYNCA KEAMEACPVS
AITIEEA
