FER_THEAC
ID FER_THEAC Reviewed; 143 AA.
AC P00218; Q9V304;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Zinc-containing ferredoxin;
GN Name=zfx; OrderedLocusNames=Ta1448;
OS Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
OS 15155 / AMRC-C165).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Thermoplasmataceae; Thermoplasma.
OX NCBI_TaxID=273075;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165;
RX PubMed=11029001; DOI=10.1038/35035069;
RA Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT acidophilum.";
RL Nature 407:508-513(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND MASS SPECTROMETRY.
RC STRAIN=HO-62;
RX PubMed=10438486; DOI=10.1074/jbc.274.33.23160;
RA Cosper N.J., Stalhandske C.M.V., Iwasaki H., Oshima T., Scott R.A.,
RA Iwasaki T.;
RT "Structural conservation of the isolated zinc site in archaeal zinc-
RT containing ferredoxins as revealed by X-ray absorption spectroscopic
RT analysis and its evolutionary implications.";
RL J. Biol. Chem. 274:23160-23168(1999).
RN [3]
RP PROTEIN SEQUENCE OF 2-143.
RC STRAIN=ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165;
RA Wakabayashi S., Fujimoto N., Wada K., Matsubara H., Kerscher L.,
RA Oesterhelt D.;
RT "Amino acid sequence of a ferredoxin from thermoacidophilic archaebacteria,
RT Thermoplasma acidophilum.";
RL FEBS Lett. 162:21-24(1983).
RN [4]
RP PROTEIN SEQUENCE OF 2-16, AND CHARACTERIZATION.
RC STRAIN=HO-62;
RX PubMed=9013590; DOI=10.1074/jbc.272.6.3453;
RA Iwasaki T., Suzuki T., Kon T., Imai T., Urushiyama A., Ohmori D.,
RA Oshima T.;
RT "Novel zinc-containing ferredoxin family in thermoacidophilic archaea.";
RL J. Biol. Chem. 272:3453-3458(1997).
CC -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons
CC in a wide variety of metabolic reactions.
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC Note=Binds 1 [3Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Note=Binds 1 [4Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Note=Binds 1 zinc ion.;
CC -!- MASS SPECTROMETRY: Mass=15961; Mass_error=10; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10438486};
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DR EMBL; AL445067; CAC12568.1; -; Genomic_DNA.
DR EMBL; AB023294; BAA82797.1; -; Genomic_DNA.
DR PIR; T37333; FEYTA.
DR RefSeq; WP_052295737.1; NC_002578.1.
DR AlphaFoldDB; P00218; -.
DR SMR; P00218; -.
DR STRING; 273075.Ta1448m; -.
DR EnsemblBacteria; CAC12568; CAC12568; CAC12568.
DR GeneID; 1457113; -.
DR KEGG; tac:Ta1448; -.
DR eggNOG; arCOG04548; Archaea.
DR HOGENOM; CLU_1507315_0_0_2; -.
DR OMA; CMACESV; -.
DR Proteomes; UP000001024; Chromosome.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR009157; Fd_Zn-bd.
DR Pfam; PF00037; Fer4; 2.
DR PIRSF; PIRSF000068; Zn_Fdx_Sulfol; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 3Fe-4S; 4Fe-4S; Direct protein sequencing; Electron transport; Iron;
KW Iron-sulfur; Metal-binding; Reference proteome; Repeat; Transport; Zinc.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9013590, ECO:0000269|Ref.3"
FT CHAIN 2..143
FT /note="Zinc-containing ferredoxin"
FT /id="PRO_0000159182"
FT DOMAIN 60..89
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 115..143
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT REGION 13..60
FT /note="N-terminal extension"
FT BINDING 31
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 58
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 69
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000305"
FT BINDING 75
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000305"
FT BINDING 79
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 117
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 124
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 127
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 130
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 134
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000305"
FT VARIANT 102
FT /note="Q -> E (in strain: HO-62)"
FT VARIANT 106
FT /note="E -> A (in strain: HO-62)"
SQ SEQUENCE 143 AA; 16093 MW; 5DA840A7856B165D CRC64;
MVKLEELDFK PKPIDEHFLE NDKDYPVTGQ HNGHDVRAEG MQRLDADGKP YPTKLGIHGT
HVAVDWDCCI ADGACMDVCP VNLYEWNLNP GKSGTGNDHK IQKGSEEWNK YRTDKCDPVR
ESDCIFCMAC ESVCPVRAIK ITP
