FG2KO_SOYBN
ID FG2KO_SOYBN Reviewed; 464 AA.
AC I1LCI8;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=UDP-glycosyltransferase 79A6 {ECO:0000303|PubMed:24072327};
DE EC=2.4.1.159 {ECO:0000305};
DE AltName: Full=Flavonol 3-O-glucoside (1->6) rhamnosyltransferase {ECO:0000303|PubMed:24072327};
DE Short=GmF3G6''Rt-a {ECO:0000303|PubMed:24072327};
GN Name=FG2 {ECO:0000303|PubMed:24072327};
GN Synonyms=UGT79A6 {ECO:0000303|PubMed:24072327};
GN OrderedLocusNames=Glyma10g33790;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000312|Proteomes:UP000008827};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Koganejiro;
RX PubMed=24072327; DOI=10.1007/s11103-013-0133-1;
RA Rojas Rodas F., Rodriguez T.O., Murai Y., Iwashina T., Sugawara S.,
RA Suzuki M., Nakabayashi R., Yonekura-Sakakibara K., Saito K., Kitajima J.,
RA Toda K., Takahashi R.;
RT "Linkage mapping, molecular cloning and functional analysis of soybean gene
RT Fg2 encoding flavonol 3-O-glucoside (1->6) rhamnosyltransferase.";
RL Plant Mol. Biol. 84:287-300(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
CC -!- FUNCTION: Flavonol 3-O-glucoside (1->6) rhamnosyltransferase converting
CC kaempferol 3-O-glucoside to kaempferol 3-O-rutinoside and utilizing 3-
CC O-glucosylated/galactosylated flavonols and UDP-rhamnose as substrates.
CC Prefers kaempferol to quercetin as an aglycon, and 3-O-galactosides to
CC 3-O-glucosides as a glycosylation pattern.
CC {ECO:0000269|PubMed:24072327}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol 3-O-beta-D-glucoside + UDP-beta-L-rhamnose = a
CC flavonol 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] + H(+) +
CC UDP; Xref=Rhea:RHEA:22528, ChEBI:CHEBI:15378, ChEBI:CHEBI:16816,
CC ChEBI:CHEBI:17952, ChEBI:CHEBI:58223, ChEBI:CHEBI:83836;
CC EC=2.4.1.159; Evidence={ECO:0000305};
CC -!- TISSUE SPECIFICITY: Expressed in young leaves, flowers, pods and pod
CC shells. Barely detected in seeds, roots and root nodules.
CC {ECO:0000269|PubMed:24072327}.
CC -!- MISCELLANEOUS: Flavonol glycosides (FGs) having rhamnose at the 6''-
CC position of glucose or galactose that is bound to the 3-position of
CC kaempferol are present in cv. Koganejiro, whereas FGs of cv.
CC Kitakomachi are devoid of rhamnose. {ECO:0000269|PubMed:24072327}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB828193; BAN91401.1; -; mRNA.
DR EMBL; CM000843; KRH34605.1; -; Genomic_DNA.
DR RefSeq; NP_001275524.1; NM_001288595.1.
DR AlphaFoldDB; I1LCI8; -.
DR SMR; I1LCI8; -.
DR STRING; 3847.GLYMA10G33790.1; -.
DR PRIDE; I1LCI8; -.
DR EnsemblPlants; KRH34605; KRH34605; GLYMA_10G194000.
DR GeneID; 100819964; -.
DR Gramene; KRH34605; KRH34605; GLYMA_10G194000.
DR KEGG; gmx:100819964; -.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_2_3_1; -.
DR InParanoid; I1LCI8; -.
DR OMA; AACHNAC; -.
DR OrthoDB; 508327at2759; -.
DR Proteomes; UP000008827; Chromosome 10.
DR GO; GO:0047230; F:flavonol-3-O-glucoside L-rhamnosyltransferase activity; IDA:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..464
FT /note="UDP-glycosyltransferase 79A6"
FT /id="PRO_0000436355"
SQ SEQUENCE 464 AA; 52244 MW; 3D1D650968989455 CRC64;
MPSELAMNND ELHVVMFPFL AFGHISPFVQ LSNKLFSHGV HVTFLSAASN IPRIRSTLNL
NPAINVISLK FPNGITNTAE LPPHLAGNLI HALDLTQDQV KSLLLELKPH YVFFDFAQHW
LPKLASEVGI KSVHFSVYSA ISDAYITVPS RFADVEGRNI TFEDLKKPPP GYPQNSNISL
KAFEAMDFMF LFTRFGEKNL TGYERVLQSL GECSFIVFKT CKEIEGPYLD YIETQFRKPV
LLSGPLVPEP STDVLEEKWS KWLDGFPAKS VILCSFGSET FLSDYQIKEL ASGLELTGLP
FILVLNFPSN LSAKAELERA LPKGYLERVK NRGVVHSGWF QQQLVLKHSS VGCYVCHGGF
SSVIEAMVNE CQLVLLPFKG DQFFNSKLIA NDLKAGVEVN RSDEDGFFHK EDILEALKTV
MLEDNKEQGK QIRENHMQWS KFLSNKEIQN KFITDLVAQL KSMA
