ID   FG3N_SOYBN              Reviewed;         459 AA.
AC   A0A0G4DBR5;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=UDP-glycosyltransferase 79B30 {ECO:0000303|PubMed:26002063};
DE            EC=2.4.1.239 {ECO:0000305};
DE   AltName: Full=Flavonol 3-O-glucoside/galactoside (1->2) glucosyltransferase {ECO:0000303|PubMed:26002063};
DE            Short=GmF3G2''Gt-a {ECO:0000303|PubMed:26002063};
GN   Name=FG3 {ECO:0000303|PubMed:26002063};
GN   Synonyms=UGT79B30 {ECO:0000303|PubMed:26002063};
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847 {ECO:0000312|EMBL:BAR88077.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, 3D-STRUCTURE
RP   MODELING, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nezumisaya;
RX   PubMed=26002063; DOI=10.1186/s12870-015-0504-7;
RA   Di S., Yan F., Rodas F.R., Rodriguez T.O., Murai Y., Iwashina T.,
RA   Sugawara S., Mori T., Nakabayashi R., Yonekura-Sakakibara K., Saito K.,
RA   Takahashi R.;
RT   "Linkage mapping, molecular cloning and functional analysis of soybean gene
RT   Fg3 encoding flavonol 3-O-glucoside/galactoside (1->2)
RT   glucosyltransferase.";
RL   BMC Plant Biol. 15:126-126(2015).
CC   -!- FUNCTION: Flavonol 3-O-glucoside/galactoside (1->2) glucosyltransferase
CC       converting kaempferol 3-O-glucoside to kaempferol 3-O-sophoroside. Has
CC       a broad in vitro activity for kaempferol/quercetin 3-O-
CC       glucoside/galactoside derivatives, but cannot glucosylate kaempferol 3-
CC       O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)- glucoside] and 3-O-rhamnosyl-
CC       (1->4)-[glucosyl-(1->6)-glucoside]. Has a higher preference for UDP-
CC       glucose than UDP-galactose, and no activity with UDP-arabinose and UDP-
CC       glucuronic acid. Represents probably a dominant allele of the gene.
CC       {ECO:0000269|PubMed:26002063}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a flavonol 3-O-beta-D-glucoside + UDP-alpha-D-glucose = a
CC         flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside + H(+) + UDP;
CC         Xref=Rhea:RHEA:18893, ChEBI:CHEBI:15378, ChEBI:CHEBI:16816,
CC         ChEBI:CHEBI:52142, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC         EC=2.4.1.239; Evidence={ECO:0000305};
CC   -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:26002063}.
CC   -!- MISCELLANEOUS: Flavonol glycosides (FGs) with glucose at the 2''-
CC       position of glucose or galactose at the 3-position of kaempferol are
CC       present in cv. Nezumisaya and absent in cv. Harosoy, whereas FGs with
CC       glucose at the 6''-position of glucose or galactose at the 3''-position
CC       of kaempferol are present in cv. Harosoy and absent in cv. Nezumisaya.
CC       {ECO:0000269|PubMed:26002063}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; LC017844; BAR88077.1; -; mRNA.
DR   AlphaFoldDB; A0A0G4DBR5; -.
DR   SMR; A0A0G4DBR5; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0033838; F:flavonol-3-O-glucoside glucosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..459
FT                   /note="UDP-glycosyltransferase 79B30"
FT                   /id="PRO_0000436353"
SQ   SEQUENCE   459 AA;  51117 MW;  75D50AC92403A3CE CRC64;
     MKSRPLHIAM YPWLAMGHQI AFLHLCNKLA IRGHKISFIT PPKAQAKLEP FNLHPNSITF
     VTINVPHVEG LPPDAQTTAD VTYPLQPQIM TAMDLTKDDI ETLLTGLKPD LVFYDFTHWM
     PALAKRLGIK AVHYCTASSV MIGYTLTPAR FHQGTDLMES DLMEPPEGYP DSSIKLQTHE
     ARVFAAKRKD TFGSNVLFYD RQFIALNEAD LLAYRTCREI EGPYMDYIGK QFNKPVVATG
     PVILDPPTLD LEEKFSTWLG GFEPGSVVYC CFGSECTLRP NQFLELVLGL ELTGMPFLAA
     VKAPLGFETV ESAMPEGFQE RVKGRGFVYG GWVQQQLILA HPSVGCFITH CGSGSLSEAL
     VNKCQLVLLP NVGDQILNAR MMGTNLEVGV EVEKGDEDGM YTKESVCKAV SIVMDCENET
     SKRVRANHAR IRELLLNKDL ESSYVDSFCM RLQEIVEGI