ID   AKA14_RAT               Reviewed;         502 AA.
AC   O35817;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=A-kinase anchor protein 14;
DE            Short=AKAP-14;
DE   AltName: Full=TAKAP-1.2;
DE   AltName: Full=Testis-specific A-kinase-anchoring protein TAKAP-80;
GN   Name=Akap14; Synonyms=Takap80;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Testis;
RX   PubMed=9208934; DOI=10.1111/j.1432-1033.1997.t01-1-00425.x;
RA   Mei X., Singh I.S., Erlichman J., Orr G.A.;
RT   "Cloning and characterization of a testis-specific, developmentally
RT   regulated A-kinase anchoring protein (TAKAP-80) present on the fibrous
RT   sheath of rat sperm.";
RL   Eur. J. Biochem. 246:425-432(1997).
CC   -!- FUNCTION: Binds to type II regulatory subunits of protein kinase A and
CC       anchors/targets them.
CC   -!- SUBUNIT: Binds to type II regulatory subunits (RII).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Note=In fibrous sheath
CC       from epididymal sperm.
CC   -!- TISSUE SPECIFICITY: Testis specific. {ECO:0000269|PubMed:9208934}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at detectable levels 30 days after birth
CC       and increased greatly during the next 10 days.
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DR   EMBL; AJ002474; CAA05485.1; -; mRNA.
DR   RefSeq; NP_067735.1; NM_021703.1.
DR   AlphaFoldDB; O35817; -.
DR   STRING; 10116.ENSRNOP00000009123; -.
DR   PaxDb; O35817; -.
DR   PRIDE; O35817; -.
DR   Ensembl; ENSRNOT00000009123; ENSRNOP00000009123; ENSRNOG00000006899.
DR   GeneID; 60332; -.
DR   KEGG; rno:60332; -.
DR   UCSC; RGD:708510; rat.
DR   CTD; 158798; -.
DR   RGD; 708510; Akap14.
DR   eggNOG; ENOG502S0CR; Eukaryota.
DR   GeneTree; ENSGT00390000003444; -.
DR   HOGENOM; CLU_542857_0_0_1; -.
DR   InParanoid; O35817; -.
DR   OMA; IRFREQW; -.
DR   OrthoDB; 1266963at2759; -.
DR   TreeFam; TF329057; -.
DR   PRO; PR:O35817; -.
DR   Proteomes; UP000002494; Chromosome X.
DR   Bgee; ENSRNOG00000006899; Expressed in testis and 2 other tissues.
DR   Genevisible; O35817; RN.
DR   GO; GO:0005930; C:axoneme; ISO:RGD.
DR   GO; GO:0005952; C:cAMP-dependent protein kinase complex; ISO:RGD.
DR   GO; GO:0035686; C:sperm fibrous sheath; IDA:RGD.
DR   GO; GO:0051018; F:protein kinase A binding; IPI:RGD.
DR   GO; GO:0034237; F:protein kinase A regulatory subunit binding; ISO:RGD.
DR   GO; GO:0007283; P:spermatogenesis; IEP:RGD.
DR   InterPro; IPR025663; AKAP_28.
DR   Pfam; PF14469; AKAP28; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat.
FT   CHAIN           1..502
FT                   /note="A-kinase anchor protein 14"
FT                   /id="PRO_0000064520"
FT   REPEAT          199..202
FT                   /note="1"
FT   REPEAT          205..208
FT                   /note="2"
FT   REPEAT          211..214
FT                   /note="3"
FT   REPEAT          217..220
FT                   /note="4"
FT   REPEAT          235..238
FT                   /note="5"
FT   REPEAT          241..244
FT                   /note="6"
FT   REPEAT          250..253
FT                   /note="7"
FT   REPEAT          262..265
FT                   /note="8"
FT   REGION          1..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          199..265
FT                   /note="8 X 4 AA repeat of P-X-X-P"
FT   REGION          258..378
FT                   /note="RII-binding"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..84
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   502 AA;  55756 MW;  D06DED22F954E975 CRC64;
     MNNKKKPPNQ KAKTVENTST AKAKKPEAPE PKNVTEPKDS KAVEDPKKSV TNQKEKKTVT
     IIADPKDKKS VTEPKEKKTV TISDPKDKKP VIEKDKRTVT FVTDVKDKRI VTDTKENRIA
     SIDTKEKGPV VPEVKEKKVQ LIDPKEKVVA PVAPVAPVAH AAQVPHAAQV PHAVQVPHAV
     PVAHAAPVAH TAQVSHAAPV APSLPVVPPA PVVPLAPVVP PAPVAHTEPV AHAVPFAPPA
     PVAPPASVAP PVPDAPVALV APVAPQVKQK IRVSEVKRKK EVPPRVPQLK EMKVVLFTPE
     IKEKKVVSEI KEKTQVIDGK EKLIVPDVKE KKPAVVEKKD ERMNEIARTV VEGVLAASVQ
     FVEEARNPIK NIKWLTHGEF TAEKGRKQIE KFVSTWEFQN RWVYYADFIE KKDLIHSFHY
     IYRVRWSAPT AVRPMARVSA NAYFTIKFNK SKPADMPVDV SYIFENSELL QRPGKIRFRE
     QWLRDITETK HILLESIPFK VV