AKA28_HUMAN
ID AKA28_HUMAN Reviewed; 197 AA.
AC Q86UN6; A6NNZ0; Q86UN4; Q86UN5;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=A-kinase anchor protein 14;
DE Short=AKAP-14;
DE AltName: Full=A-kinase anchor protein 28 kDa;
DE Short=AKAP 28;
DE AltName: Full=Protein kinase A-anchoring protein 14;
DE Short=PRKA14;
GN Name=AKAP14; Synonyms=AKAP28;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBUNIT, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF LEU-43 AND VAL-47.
RC TISSUE=Lung epithelium;
RX PubMed=12475942; DOI=10.1091/mbc.e02-07-0391;
RA Kultgen P.L., Byrd S.K., Ostrowski L.E., Milgram S.L.;
RT "Characterization of an A-kinase anchoring protein in human ciliary
RT axonemes.";
RL Mol. Biol. Cell 13:4156-4166(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Binds to type II regulatory subunits of protein kinase A and
CC anchors/targets them.
CC -!- SUBUNIT: Binds to type II regulatory subunits (RII).
CC {ECO:0000269|PubMed:12475942}.
CC -!- INTERACTION:
CC Q86UN6; Q9C005: DPY30; NbExp=3; IntAct=EBI-2119626, EBI-744973;
CC Q86UN6; P31321: PRKAR1B; NbExp=3; IntAct=EBI-2119626, EBI-2805516;
CC Q86UN6; P13861-2: PRKAR2A; NbExp=3; IntAct=EBI-2119626, EBI-11752137;
CC Q86UN6; P31323: PRKAR2B; NbExp=7; IntAct=EBI-2119626, EBI-2930670;
CC Q86UN6; Q9HAT0: ROPN1; NbExp=3; IntAct=EBI-2119626, EBI-1378139;
CC Q86UN6; Q96C74: ROPN1L; NbExp=3; IntAct=EBI-2119626, EBI-9033237;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Isoform A;
CC IsoId=Q86UN6-1; Sequence=Displayed;
CC Name=2; Synonyms=Isoform B;
CC IsoId=Q86UN6-2; Sequence=VSP_009909;
CC Name=3; Synonyms=Isoform C;
CC IsoId=Q86UN6-3; Sequence=VSP_009910, VSP_009911;
CC -!- TISSUE SPECIFICITY: Present in cilia (at protein level). Expressed in
CC tissues containing axoneme-based organelles (cilia and/or flagella):
CC trachea and testis. Highly expressed in airway cilia.
CC {ECO:0000269|PubMed:12475942}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF514780; AAP20825.1; -; mRNA.
DR EMBL; AF514781; AAP20826.1; -; mRNA.
DR EMBL; AF514782; AAP20827.1; -; mRNA.
DR EMBL; AC002477; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC066357; AAH66357.1; -; mRNA.
DR CCDS; CCDS14591.1; -. [Q86UN6-1]
DR CCDS; CCDS35376.1; -. [Q86UN6-2]
DR CCDS; CCDS35377.1; -. [Q86UN6-3]
DR RefSeq; NP_001008534.1; NM_001008534.1. [Q86UN6-2]
DR RefSeq; NP_001008535.1; NM_001008535.1. [Q86UN6-3]
DR RefSeq; NP_848928.1; NM_178813.5. [Q86UN6-1]
DR AlphaFoldDB; Q86UN6; -.
DR SMR; Q86UN6; -.
DR BioGRID; 127707; 17.
DR IntAct; Q86UN6; 15.
DR STRING; 9606.ENSP00000360485; -.
DR iPTMnet; Q86UN6; -.
DR PhosphoSitePlus; Q86UN6; -.
DR BioMuta; AKAP14; -.
DR DMDM; 46395790; -.
DR MassIVE; Q86UN6; -.
DR PaxDb; Q86UN6; -.
DR PeptideAtlas; Q86UN6; -.
DR PRIDE; Q86UN6; -.
DR ProteomicsDB; 1648; -.
DR ProteomicsDB; 69835; -. [Q86UN6-1]
DR ProteomicsDB; 69836; -. [Q86UN6-2]
DR ProteomicsDB; 69837; -. [Q86UN6-3]
DR Antibodypedia; 44608; 104 antibodies from 26 providers.
DR DNASU; 158798; -.
DR Ensembl; ENST00000334356.2; ENSP00000334680.2; ENSG00000186471.13. [Q86UN6-2]
DR Ensembl; ENST00000371422.5; ENSP00000360476.1; ENSG00000186471.13. [Q86UN6-3]
DR Ensembl; ENST00000371425.8; ENSP00000360479.4; ENSG00000186471.13. [Q86UN6-2]
DR Ensembl; ENST00000371431.8; ENSP00000360485.3; ENSG00000186471.13. [Q86UN6-1]
DR GeneID; 158798; -.
DR KEGG; hsa:158798; -.
DR MANE-Select; ENST00000371431.8; ENSP00000360485.3; NM_178813.6; NP_848928.1.
DR UCSC; uc004ese.4; human. [Q86UN6-1]
DR CTD; 158798; -.
DR GeneCards; AKAP14; -.
DR HGNC; HGNC:24061; AKAP14.
DR HPA; ENSG00000186471; Group enriched (fallopian tube, testis).
DR MIM; 300462; gene.
DR neXtProt; NX_Q86UN6; -.
DR OpenTargets; ENSG00000186471; -.
DR PharmGKB; PA134914893; -.
DR VEuPathDB; HostDB:ENSG00000186471; -.
DR eggNOG; ENOG502S0CR; Eukaryota.
DR GeneTree; ENSGT00390000003444; -.
DR HOGENOM; CLU_116552_0_0_1; -.
DR InParanoid; Q86UN6; -.
DR OMA; FRVRWSI; -.
DR OrthoDB; 1552956at2759; -.
DR PhylomeDB; Q86UN6; -.
DR TreeFam; TF329057; -.
DR PathwayCommons; Q86UN6; -.
DR SignaLink; Q86UN6; -.
DR BioGRID-ORCS; 158798; 9 hits in 698 CRISPR screens.
DR ChiTaRS; AKAP14; human.
DR GenomeRNAi; 158798; -.
DR Pharos; Q86UN6; Tbio.
DR PRO; PR:Q86UN6; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q86UN6; protein.
DR Bgee; ENSG00000186471; Expressed in bronchial epithelial cell and 114 other tissues.
DR Genevisible; Q86UN6; HS.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005952; C:cAMP-dependent protein kinase complex; IMP:UniProtKB.
DR GO; GO:0034237; F:protein kinase A regulatory subunit binding; IPI:UniProtKB.
DR InterPro; IPR025663; AKAP_28.
DR Pfam; PF14469; AKAP28; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..197
FT /note="A-kinase anchor protein 14"
FT /id="PRO_0000064521"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 35..52
FT /note="RII-binding"
FT VAR_SEQ 88..147
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12475942"
FT /id="VSP_009909"
FT VAR_SEQ 88..89
FT /note="KC -> VS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12475942"
FT /id="VSP_009910"
FT VAR_SEQ 90..197
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12475942"
FT /id="VSP_009911"
FT MUTAGEN 43
FT /note="L->P: Abolishes RII-binding; when associated with P-
FT 47."
FT /evidence="ECO:0000269|PubMed:12475942"
FT MUTAGEN 47
FT /note="V->P: Abolishes RII-binding; when associated with P-
FT 43."
FT /evidence="ECO:0000269|PubMed:12475942"
FT CONFLICT Q86UN6-2:87
FT /note="S -> SP (in Ref. 1; AAP20826)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 197 AA; 22815 MW; 3A76B9C3036EAEB9 CRC64;
MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN
PLKNIKWMTH GEFTVEKGLK QIDEYFSKCV SKKCWAHGVE FVERKDLIHS FLYIYYVHWS
ISTADLPVAR ISAGTYFTMK VSKTKPPDAP IVVSYVGDHQ ALVHRPGMVR FRENWQKNLT
DAKYSFMESF PFLFNRV
