AKA7A_MOUSE
ID AKA7A_MOUSE Reviewed; 81 AA.
AC O55074; G3X9Q5;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 4.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=A-kinase anchor protein 7 isoform alpha;
DE Short=AKAP-7 isoform alpha;
DE AltName: Full=A-kinase anchor protein 18;
DE Short=AKAP-18;
DE AltName: Full=A-kinase anchor protein 9 kDa;
DE Short=AKAP 9;
DE AltName: Full=Protein kinase A-anchoring protein 7 isoform alpha;
DE Short=PRKA7 isoform alpha;
GN Name=Akap7; Synonyms=Akap18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2,
RP AND PALMITOYLATION AT CYS-5 AND CYS-6.
RX PubMed=9545239; DOI=10.1093/emboj/17.8.2261;
RA Fraser I.D.C., Tavalin S.J., Lester L.B., Langeberg L.K., Westphal A.M.,
RA Dean R.A., Marrion N.V., Scott J.D.;
RT "A novel lipid-anchored A-kinase anchoring protein facilitates cAMP-
RT responsive membrane events.";
RL EMBO J. 17:2261-2272(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=9620705; DOI=10.1016/s0896-6273(00)80482-1;
RA Gray P.C., Johnson B.D., Westenbroek R.E., Hays L.G., Yates J.R. III,
RA Scheuer T., Catterall W.A., Murphy B.J.;
RT "Primary structure and function of an A kinase anchoring protein associated
RT with calcium channels.";
RL Neuron 20:1017-1026(1998).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Targets the cAMP-dependent protein kinase (PKA) to the plasma
CC membrane, and permits functional coupling to the L-type calcium
CC channel. The membrane-associated form reduces epithelial sodium channel
CC (ENaC) activity, whereas the free cytoplasmic form may negatively
CC regulate ENaC channel feedback inhibition by intracellular sodium (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:9620705}.
CC -!- SUBUNIT: Binds cAMP-dependent protein kinase (PKA). Interacts with
CC PRKCA; only the cytoplasmic form is capable of interacting with PRKCA
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lateral cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000269|PubMed:9545239}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Alpha;
CC IsoId=O55074-1; Sequence=Displayed;
CC Name=Gamma;
CC IsoId=Q7TN79-1; Sequence=External;
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DR EMBL; AF047716; AAC40085.1; -; mRNA.
DR EMBL; AC153549; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC153550; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466540; EDL04795.1; -; Genomic_DNA.
DR CCDS; CCDS83688.1; -. [O55074-1]
DR RefSeq; NP_001334389.1; NM_001347460.1. [O55074-1]
DR AlphaFoldDB; O55074; -.
DR SMR; O55074; -.
DR BioGRID; 240608; 1.
DR iPTMnet; O55074; -.
DR PhosphoSitePlus; O55074; -.
DR SwissPalm; O55074; -.
DR PeptideAtlas; O55074; -.
DR PRIDE; O55074; -.
DR ProteomicsDB; 296149; -. [O55074-1]
DR Antibodypedia; 19674; 297 antibodies from 28 providers.
DR DNASU; 432442; -.
DR Ensembl; ENSMUST00000100012; ENSMUSP00000097590; ENSMUSG00000039166. [O55074-1]
DR GeneID; 432442; -.
DR KEGG; mmu:432442; -.
DR UCSC; uc007erh.1; mouse. [O55074-1]
DR CTD; 9465; -.
DR MGI; MGI:1859150; Akap7.
DR VEuPathDB; HostDB:ENSMUSG00000039166; -.
DR GeneTree; ENSGT00390000012756; -.
DR HOGENOM; CLU_177905_0_0_1; -.
DR OrthoDB; 606751at2759; -.
DR BioGRID-ORCS; 432442; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Akap7; mouse.
DR Proteomes; UP000000589; Chromosome 10.
DR Bgee; ENSMUSG00000039166; Expressed in animal zygote and 224 other tissues.
DR ExpressionAtlas; O55074; baseline and differential.
DR Genevisible; O55074; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0070382; C:exocytic vesicle; ISO:MGI.
DR GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; IDA:SynGO.
DR GO; GO:0016328; C:lateral plasma membrane; ISO:MGI.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0016529; C:sarcoplasmic reticulum; ISO:MGI.
DR GO; GO:0030315; C:T-tubule; ISO:MGI.
DR GO; GO:0016208; F:AMP binding; ISO:MGI.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0019904; F:protein domain specific binding; ISO:MGI.
DR GO; GO:0051018; F:protein kinase A binding; ISS:UniProtKB.
DR GO; GO:0034237; F:protein kinase A regulatory subunit binding; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; IDA:MGI.
DR GO; GO:0071320; P:cellular response to cAMP; ISO:MGI.
DR GO; GO:0050804; P:modulation of chemical synaptic transmission; IDA:SynGO.
DR GO; GO:1902261; P:positive regulation of delayed rectifier potassium channel activity; ISO:MGI.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; ISO:MGI.
DR GO; GO:0008104; P:protein localization; ISO:MGI.
DR GO; GO:0060306; P:regulation of membrane repolarization; ISO:MGI.
DR GO; GO:0010738; P:regulation of protein kinase A signaling; ISO:MGI.
DR GO; GO:0007178; P:transmembrane receptor protein serine/threonine kinase signaling pathway; ISO:MGI.
DR InterPro; IPR019511; AKAP7_RI-RII-bd_dom.
DR Pfam; PF10470; AKAP7_RIRII_bdg; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Lipoprotein; Membrane; Myristate;
KW Palmitate; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..81
FT /note="A-kinase anchor protein 7 isoform alpha"
FT /id="PRO_0000064532"
FT REGION 2..11
FT /note="Required for membrane localization"
FT /evidence="ECO:0000250"
FT REGION 29..42
FT /note="RII-binding"
FT /evidence="ECO:0000250"
FT REGION 49..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..81
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000269|PubMed:9545239"
FT LIPID 5
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:9545239"
FT LIPID 6
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:9545239"
FT CONFLICT 12
FT /note="D -> E (in Ref. 1; AAC40085)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="R -> K (in Ref. 1; AAC40085)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="T -> V (in Ref. 1; AAC40085)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 81 AA; 9169 MW; 2297FA55FE35AC9E CRC64;
MGQLCCFPFA RDEGKICEKD RREPEDAELV RLSKRLVENA VLKAVQQYLE ETQNKKQPGE
GNSTKAEEGD RNGDGSDNNR K
