ID   AKAI1_HUMAN             Reviewed;          69 AA.
AC   P0CW23;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=A-kinase anchor protein inhibitor 1;
GN   Name=AKAIN1 {ECO:0000312|HGNC:HGNC:28285}; Synonyms=C18orf42;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Subthalamic nucleus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16177791; DOI=10.1038/nature03983;
RA   Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA   Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA   Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA   Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA   Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA   Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA   Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA   Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA   Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 18.";
RL   Nature 437:551-555(2005).
RN   [3]
RP   TISSUE SPECIFICITY, SUBUNIT, MUTAGENESIS OF ILE-25, AND FUNCTION.
RX   PubMed=25653177; DOI=10.1111/gtc.12217;
RA   Fukuda M., Aizawa Y.;
RT   "Hypothetical gene C18orf42 encodes a novel protein kinase A-binding
RT   protein.";
RL   Genes Cells 20:267-280(2015).
CC   -!- FUNCTION: Protein kinase A (PKA)-binding protein. Binds to type II
CC       regulatory subunits of protein kinase A (PKA) and may block the A-
CC       kinase anchoring protein (AKAP)-mediated subcellular localization of
CC       PKA (PubMed:25653177). {ECO:0000269|PubMed:25653177}.
CC   -!- SUBUNIT: Binds cAMP-dependent protein kinase (PKA). Interacts
CC       specifically with RII-regulatory subunits of PKA (PRKAR2A and PRKAR2B)
CC       (PubMed:25653177). {ECO:0000269|PubMed:25653177}.
CC   -!- INTERACTION:
CC       P0CW23; Q9HAT0: ROPN1; NbExp=3; IntAct=EBI-18397742, EBI-1378139;
CC   -!- TISSUE SPECIFICITY: Preferentially expressed in the neural tissues
CC       (PubMed:25653177). {ECO:0000269|PubMed:25653177}.
CC   -!- DOMAIN: The RII-alpha binding site, predicted to form an amphipathic
CC       helix, could participate in protein-protein interactions with a
CC       complementary surface on the R-subunit dimer.
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DR   EMBL; DA365827; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AP001496; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP005380; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS54179.1; -.
DR   RefSeq; NP_001138666.1; NM_001145194.1.
DR   AlphaFoldDB; P0CW23; -.
DR   SMR; P0CW23; -.
DR   BioGRID; 568101; 1.
DR   IntAct; P0CW23; 1.
DR   STRING; 9606.ENSP00000399075; -.
DR   BioMuta; AKAIN1; -.
DR   DMDM; 338817804; -.
DR   MassIVE; P0CW23; -.
DR   PaxDb; P0CW23; -.
DR   PeptideAtlas; P0CW23; -.
DR   PRIDE; P0CW23; -.
DR   ProteomicsDB; 52522; -.
DR   Antibodypedia; 62516; 10 antibodies from 8 providers.
DR   DNASU; 642597; -.
DR   Ensembl; ENST00000434239.4; ENSP00000399075.3; ENSG00000231824.4.
DR   GeneID; 642597; -.
DR   KEGG; hsa:642597; -.
DR   MANE-Select; ENST00000434239.4; ENSP00000399075.3; NM_001145194.2; NP_001138666.1.
DR   UCSC; uc010wzc.2; human.
DR   CTD; 642597; -.
DR   DisGeNET; 642597; -.
DR   GeneCards; AKAIN1; -.
DR   HGNC; HGNC:28285; AKAIN1.
DR   HPA; ENSG00000231824; Tissue enhanced (adrenal gland, brain).
DR   MIM; 616427; gene.
DR   neXtProt; NX_P0CW23; -.
DR   OpenTargets; ENSG00000231824; -.
DR   VEuPathDB; HostDB:ENSG00000231824; -.
DR   eggNOG; ENOG502SEI0; Eukaryota.
DR   GeneTree; ENSGT00560000078638; -.
DR   HOGENOM; CLU_2782380_0_0_1; -.
DR   InParanoid; P0CW23; -.
DR   OMA; CELTKKH; -.
DR   PhylomeDB; P0CW23; -.
DR   PathwayCommons; P0CW23; -.
DR   SignaLink; P0CW23; -.
DR   BioGRID-ORCS; 642597; 5 hits in 1005 CRISPR screens.
DR   GenomeRNAi; 642597; -.
DR   Pharos; P0CW23; Tdark.
DR   PRO; PR:P0CW23; -.
DR   Proteomes; UP000005640; Chromosome 18.
DR   RNAct; P0CW23; protein.
DR   Bgee; ENSG00000231824; Expressed in cerebellar hemisphere and 94 other tissues.
DR   ExpressionAtlas; P0CW23; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0051018; F:protein kinase A binding; IDA:UniProtKB.
DR   GO; GO:0031333; P:negative regulation of protein-containing complex assembly; IEA:Ensembl.
DR   GO; GO:0008104; P:protein localization; IDA:UniProtKB.
DR   GO; GO:0065003; P:protein-containing complex assembly; IEA:Ensembl.
DR   InterPro; IPR019511; AKAP7_RI-RII-bd_dom.
DR   Pfam; PF10470; AKAP7_RIRII_bdg; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..69
FT                   /note="A-kinase anchor protein inhibitor 1"
FT                   /id="PRO_0000410771"
FT   REGION          17..33
FT                   /note="PKA-RII subunit binding domain"
FT                   /evidence="ECO:0000305"
FT   REGION          37..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        40..69
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         25
FT                   /note="I->P: Reduces binding to RII subunits of PKA."
FT                   /evidence="ECO:0000269|PubMed:25653177"
SQ   SEQUENCE   69 AA;  7848 MW;  BB4ED950347AD8C5 CRC64;
     MVFAPGEKPG NEPEEVKLQN ASKQIVQNAI LQAVQQVSQE SQRREERISD NRDHIQLGVG
     ELTKKHEKK