FGSR_GIBZE
ID FGSR_GIBZE Reviewed; 492 AA.
AC I1RC73; A0A098D5P4;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Zn(2)-C6 fungal-type transcription factor {ECO:0000303|PubMed:30874562};
GN Name=SR {ECO:0000303|PubMed:30874562}; ORFNames=FG01176, FGRAMPH1_01T02919;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, PHOSPHORYLATION AT
RP THR-82; SER-92; SER-102; THR-217 AND SER-305, MUTAGENESIS OF THR-82;
RP SER-92; SER-102; THR-217 AND SER-305, AND INTERACTION WITH HOG1.
RX PubMed=30874562; DOI=10.1038/s41467-019-09145-6;
RA Liu Z., Jian Y., Chen Y., Kistler H.C., He P., Ma Z., Yin Y.;
RT "A phosphorylated transcription factor regulates sterol biosynthesis in
RT Fusarium graminearum.";
RL Nat. Commun. 10:1228-1228(2019).
CC -!- FUNCTION: Transcription factor that targets gene promoters containing 2
CC conserved CGAA repeat sequences (PubMed:30874562). Positively regulates
CC the expression of ergosterol biosynthesis genes including CYP51A and
CC CYP51B encoding the sterol 14-alpha demethylase, and ERG6A and ERG6B
CC encoding the sterol 24-C-methyltransferase (PubMed:30874562).
CC {ECO:0000269|PubMed:30874562}.
CC -!- SUBUNIT: Interacts with HOG1. {ECO:0000269|PubMed:30874562}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:30874562}.
CC -!- PTM: Phosphorylation at Thr-82, Ser-92, Ser-102, thr-117 and ser-305 by
CC HOG1 is required for regulating expression of ergosterol biosynthesis
CC genes. {ECO:0000269|PubMed:30874562}.
CC -!- DISRUPTION PHENOTYPE: Reduces the production of ergosterol
CC (PubMed:30874562). Leads to increased sensitivity to azole compounds,
CC but not to iprodione and fludioxonil that target the high osmolarity
CC glycerol (HOG) pathway (PubMed:30874562).
CC {ECO:0000269|PubMed:30874562}.
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DR EMBL; HG970332; CEF73261.1; -; Genomic_DNA.
DR RefSeq; XP_011316947.1; XM_011318645.1.
DR SMR; I1RC73; -.
DR GeneID; 23548625; -.
DR KEGG; fgr:FGSG_01176; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G02919; -.
DR eggNOG; ENOG502SP5N; Eukaryota.
DR HOGENOM; CLU_024934_6_2_1; -.
DR InParanoid; I1RC73; -.
DR PHI-base; PHI:1933; -.
DR PHI-base; PHI:6134; -.
DR Proteomes; UP000070720; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR021858; Fun_TF.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF11951; Fungal_trans_2; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..492
FT /note="Zn(2)-C6 fungal-type transcription factor"
FT /id="PRO_0000454349"
FT DNA_BIND 14..41
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 58..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..106
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 82
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:30874562"
FT MOD_RES 92
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:30874562"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:30874562"
FT MOD_RES 217
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:30874562"
FT MOD_RES 305
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 82
FT /note="T->A: Impairs phosphorylation by HOG1, blocks
FT induction of CYP51A expression and exhibits
FT hypersensitivity to tebuconazole; when associated with A-
FT 92, A-102, A-217 and A-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 82
FT /note="T->D: Mimics phosphorylation by HOG1; when
FT associated with D-92, D-102, D-217 and D-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 92
FT /note="S->A: Impairs phosphorylation by HOG1, blocks
FT induction of CYP51A expression and exhibits
FT hypersensitivity to tebuconazole; when associated with A-
FT 82, A-102, A-217 and A-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 92
FT /note="S->D: Mimics phosphorylation by HOG1; when
FT associated with D-82, D-102, D-217 and D-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 102
FT /note="S->A: Impairs phosphorylation by HOG1, blocks
FT induction of CYP51A expression and exhibits
FT hypersensitivity to tebuconazole; when associated with A-
FT 82, A-92, A-217 and A-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 102
FT /note="S->D: Mimics phosphorylation by HOG1; when
FT associated with D-82, D-92, D-217 and D-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 217
FT /note="T->A: Impairs phosphorylation by HOG1, blocks
FT induction of CYP51A expression and exhibits
FT hypersensitivity to tebuconazole; when associated with A-
FT 82, A-92, A-102 and A-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 217
FT /note="T->D: Mimics phosphorylation by HOG1; when
FT associated with D-82, D-92, D-102 and D-305."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 305
FT /note="S->A: Impairs phosphorylation by HOG1, blocks
FT induction of CYP51A expression and exhibits
FT hypersensitivity to tebuconazole; when associated with A-
FT 82, A-92, A-102 and A-217."
FT /evidence="ECO:0000269|PubMed:30874562"
FT MUTAGEN 305
FT /note="S->D: Mimics phosphorylation by HOG1; when
FT associated with D-82, D-92, D-102 and D-217."
FT /evidence="ECO:0000269|PubMed:30874562"
SQ SEQUENCE 492 AA; 54123 MW; FC67D7AEA3DCC187 CRC64;
MPPRRSHKKS RAGCRRCKNR KIKCDEVHPR CGNCAKHGVP CDFSNPDVLE ELAISTNTST
ESVGAPTPSP APTVNFNSAP RTPLTRPRAP SSPARAPRPN PSPPTSVYSQ PSISSSTNTI
DHGERMLELR LMHHYTNVTS KTLLTNSPAA EDIWQRAVPQ MAFSGNGKTY LADAILSVAA
LHLRSMSPND KALVRASHAY SASSLSAFGA SLGAGITPEN AEALFLTATL IAFQASASRI
FVKDDGDAAP GDPTVRYVPP LSWFHAFQGV KTVVANSWQW IHHSDIVKVI IDSQPSFQLN
LNPRSPDSFF GHMLEGLADE LSNEDPRLVA STTQAYSHAV SVLNWAHKNY HAAAALTFTA
TVSKRYVDLV DARRPRALAI LACFFALLKR MDNVWWLQDV ARREVMGLVS LFEPGSKWWR
HLEWPIRIAV LDGSSIPQDI WGTELEEQAP EQQNVLGSMT QHIEMFAEML NQHTQPPIPI
ADEDLIVPDS PD
