FHL5_MOUSE
ID FHL5_MOUSE Reviewed; 284 AA.
AC Q9WTX7;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Four and a half LIM domains protein 5;
DE Short=FHL-5;
DE AltName: Full=Activator of cAMP-responsive element modulator in testis;
DE Short=Activator of CREM in testis;
GN Name=Fhl5; Synonyms=Act;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP INTERACTION WITH CREM.
RC TISSUE=Testis;
RX PubMed=10086359; DOI=10.1038/18237;
RA Fimia G.M., De Cesare D., Sassone-Corsi P.;
RT "CBP-independent activation of CREM and CREB by the LIM-only protein ACT.";
RL Nature 398:165-169(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH SPAG8.
RX PubMed=20488182; DOI=10.1016/j.febslet.2010.05.016;
RA Wu H., Chen Y., Miao S., Zhang C., Zong S., Koide S.S., Wang L.;
RT "Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM
RT in testis during spermatogenesis.";
RL FEBS Lett. 584:2807-2815(2010).
CC -!- FUNCTION: May be involved in the regulation of spermatogenesis.
CC Stimulates CREM transcriptional activity in a phosphorylation-
CC independent manner (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CREM (via the third LIM domain)
CC (PubMed:10086359). Interacts (via second LIM domain) with SPAG8
CC (PubMed:20488182). {ECO:0000269|PubMed:10086359,
CC ECO:0000269|PubMed:20488182}.
CC -!- INTERACTION:
CC Q9WTX7; Q3V0Q6: Spag8; NbExp=5; IntAct=EBI-7530396, EBI-7981981;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10086359}.
CC Note=Nuclei of round and elongated spermatids.
CC -!- TISSUE SPECIFICITY: Testis-specific, temporal expression is coordinated
CC with CREM. {ECO:0000269|PubMed:10086359}.
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DR EMBL; AF083394; AAD23916.1; -; mRNA.
DR EMBL; AK006414; BAB24576.1; -; mRNA.
DR EMBL; BC048473; AAH48473.1; -; mRNA.
DR CCDS; CCDS18009.1; -.
DR RefSeq; NP_067293.1; NM_021318.3.
DR RefSeq; XP_011248382.1; XM_011250080.2.
DR AlphaFoldDB; Q9WTX7; -.
DR SMR; Q9WTX7; -.
DR BioGRID; 208318; 3.
DR IntAct; Q9WTX7; 2.
DR MINT; Q9WTX7; -.
DR STRING; 10090.ENSMUSP00000029922; -.
DR iPTMnet; Q9WTX7; -.
DR PhosphoSitePlus; Q9WTX7; -.
DR MaxQB; Q9WTX7; -.
DR PaxDb; Q9WTX7; -.
DR PRIDE; Q9WTX7; -.
DR ProteomicsDB; 266843; -.
DR Antibodypedia; 31954; 165 antibodies from 23 providers.
DR DNASU; 57756; -.
DR Ensembl; ENSMUST00000029922; ENSMUSP00000029922; ENSMUSG00000028259.
DR Ensembl; ENSMUST00000108204; ENSMUSP00000103839; ENSMUSG00000028259.
DR GeneID; 57756; -.
DR KEGG; mmu:57756; -.
DR UCSC; uc008sef.1; mouse.
DR CTD; 9457; -.
DR MGI; MGI:1913192; Fhl5.
DR VEuPathDB; HostDB:ENSMUSG00000028259; -.
DR eggNOG; KOG1704; Eukaryota.
DR GeneTree; ENSGT00950000183028; -.
DR HOGENOM; CLU_001357_2_0_1; -.
DR InParanoid; Q9WTX7; -.
DR OMA; ERCFNCA; -.
DR OrthoDB; 642235at2759; -.
DR PhylomeDB; Q9WTX7; -.
DR TreeFam; TF314113; -.
DR BioGRID-ORCS; 57756; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Fhl5; mouse.
DR PRO; PR:Q9WTX7; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9WTX7; protein.
DR Bgee; ENSMUSG00000028259; Expressed in seminiferous tubule of testis and 30 other tissues.
DR Genevisible; Q9WTX7; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0030018; C:Z disc; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0006351; P:transcription, DNA-templated; TAS:MGI.
DR CDD; cd09428; LIM2_FHL5; 1.
DR InterPro; IPR037987; FHL2/3/5.
DR InterPro; IPR042947; FHL5_LIM2.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24205; PTHR24205; 1.
DR Pfam; PF00412; LIM; 4.
DR SMART; SM00132; LIM; 4.
DR PROSITE; PS00478; LIM_DOMAIN_1; 4.
DR PROSITE; PS50023; LIM_DOMAIN_2; 4.
PE 1: Evidence at protein level;
KW LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat; Zinc;
KW Zinc-finger.
FT CHAIN 1..284
FT /note="Four and a half LIM domains protein 5"
FT /id="PRO_0000075743"
FT DOMAIN 39..100
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 101..160
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 161..220
FT /note="LIM zinc-binding 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT ZN_FING 8..32
FT /note="C4-type"
FT /evidence="ECO:0000255"
SQ SEQUENCE 284 AA; 32907 MW; 0537543FC0B222E4 CRC64;
MTSSQFDCQY CTSSLIGKKY VLKDDNLYCI SCYDRIFSNY CEQCKEPIES DSKDLCYKNR
HWHEGCFRCN KCHHSLVEKP FVAKDDRLLC TDCYSNECSS KCFHCKRTIM PGSRKMEFKG
NYWHETCFVC EHCRQPIGTK PLISKESGNY CVPCFEKEFA HYCNFCKKVI TSGGITFRDQ
IWHKECFLCS GCRKELYEEA FMSKDDFPFC LDCYNHLYAK KCAACTKPIT GLRGAKFICF
QDRQWHSECF NCGKCSVSLV GEGFLTHNME ILCRKCGSGA DTDA
