FHLA_SALTS
ID FHLA_SALTS Reviewed; 692 AA.
AC E1WAA4; P40734; Q9L6U9;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Formate hydrogenlyase transcriptional activator;
GN Name=fhlA; OrderedLocusNames=SL1344_2839;
OS Salmonella typhimurium (strain SL1344).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=216597;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SL1344;
RX PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT "The transcriptional landscape and small RNAs of Salmonella enterica
RT serovar Typhimurium.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-692.
RC STRAIN=SL1344;
RX PubMed=7783645; DOI=10.1111/j.1365-2958.1995.tb02382.x;
RA Mills D.M., Bajaj V., Lee C.A.;
RT "A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion
RT genes is absent from the corresponding region of the Escherichia coli K-12
RT chromosome.";
RL Mol. Microbiol. 15:749-759(1995).
CC -!- FUNCTION: Required for induction of expression of the formate
CC dehydrogenase H and hydrogenase-3 structural genes. {ECO:0000250}.
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DR EMBL; FQ312003; CBW18937.1; -; Genomic_DNA.
DR EMBL; U16278; AAA80577.1; -; Genomic_DNA.
DR RefSeq; WP_000122692.1; NZ_QASL01000017.1.
DR AlphaFoldDB; E1WAA4; -.
DR SMR; E1WAA4; -.
DR EnsemblBacteria; CBW18937; CBW18937; SL1344_2839.
DR KEGG; sey:SL1344_2839; -.
DR PATRIC; fig|216597.6.peg.3160; -.
DR HOGENOM; CLU_000445_95_0_6; -.
DR OMA; RMKRTID; -.
DR BioCyc; SENT216597:SL1344_RS14805-MON; -.
DR Proteomes; UP000008962; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 2.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; DNA-binding; Nucleotide-binding; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..692
FT /note="Formate hydrogenlyase transcriptional activator"
FT /id="PRO_0000406081"
FT DOMAIN 202..344
FT /note="GAF"
FT DOMAIN 381..610
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 663..682
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 409..416
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 472..481
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT CONFLICT 639
FT /note="A -> D (in Ref. 2; AAA80577)"
FT /evidence="ECO:0000305"
FT CONFLICT 691..692
FT /note="LA -> FERVAGSDRLEMMRAVAWRFPFTSCADIFPAIPERALIVDKVPHGQ
FT DRMPPKHSRTCPTHNLLHLLAFSRLIAVHRALLTAGFSSPNTHLSRRFCA (in Ref.
FT 2; AAA80577)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 692 AA; 78253 MW; DD7E43240B2BBE6D CRC64;
MSYTPMSDLG QQGLFDITRT LLQQPDLASL SEALSQLVKR SALADSAGIV LWQAQSQRAQ
YYATRENGRP VEYEDETVLA HGPVRRILSR PDALHCNFHE FTETWPQLAA SGLYPEFGHY
CLLPLAAEGR IFGGCEFIRQ EDRPWSEKEY DRLHTFTQIV GVVAEQIQNR VNNNVDYDLL
CRERDNFRIL VAITNAVLSR LDIDELVSEV AKEIHHYFNI DAISIVLRSH RKNKLNIYST
HYLDEHHPAH EQSEVDEAGT LTERVFKSKE MLLINLNERD PLAPYERMLF DTWGNQIQTL
CLLPLMSGKT MLGVLKLAQC EEKVFTTANL KLLRQIAERV AIAVDNALAY QEIHRLKERL
VDENLALTEQ LNNVDSEFGE IIGRSEAMYN VLKQVEMVAQ SDSTVLILGE TGTGKELIAR
AIHNLSGRSG RRMVKMNCAA MPAGLLESDL FGHERGAFTG ASAQRIGRFE LADKSSLFLD
EVGDMPLELQ PKLLRVLQEQ EFERLGSNKL IQTDVRLIAA TNRDLKKMVA DREFRNDLYY
RLNVFPIQLP PLRERPEDIP LLVKAFTFKI ARRMGRNIDS IPAETLRTLS SMEWPGNVRE
LENVVERAVL LTRGNVLQLS LPDITAVTPD TSPVATESAK EGEDEYQLII RVLKETNGVV
AGPKGAAQRL GLKRTTLLSR MKRLGIDKDA LA