ID   FHLA_SALTS              Reviewed;         692 AA.
AC   E1WAA4; P40734; Q9L6U9;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Formate hydrogenlyase transcriptional activator;
GN   Name=fhlA; OrderedLocusNames=SL1344_2839;
OS   Salmonella typhimurium (strain SL1344).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=216597;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SL1344;
RX   PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA   Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA   Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA   Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA   Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT   "The transcriptional landscape and small RNAs of Salmonella enterica
RT   serovar Typhimurium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-692.
RC   STRAIN=SL1344;
RX   PubMed=7783645; DOI=10.1111/j.1365-2958.1995.tb02382.x;
RA   Mills D.M., Bajaj V., Lee C.A.;
RT   "A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion
RT   genes is absent from the corresponding region of the Escherichia coli K-12
RT   chromosome.";
RL   Mol. Microbiol. 15:749-759(1995).
CC   -!- FUNCTION: Required for induction of expression of the formate
CC       dehydrogenase H and hydrogenase-3 structural genes. {ECO:0000250}.
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DR   EMBL; FQ312003; CBW18937.1; -; Genomic_DNA.
DR   EMBL; U16278; AAA80577.1; -; Genomic_DNA.
DR   RefSeq; WP_000122692.1; NZ_QASL01000017.1.
DR   AlphaFoldDB; E1WAA4; -.
DR   SMR; E1WAA4; -.
DR   EnsemblBacteria; CBW18937; CBW18937; SL1344_2839.
DR   KEGG; sey:SL1344_2839; -.
DR   PATRIC; fig|216597.6.peg.3160; -.
DR   HOGENOM; CLU_000445_95_0_6; -.
DR   OMA; RMKRTID; -.
DR   BioCyc; SENT216597:SL1344_RS14805-MON; -.
DR   Proteomes; UP000008962; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; DNA-binding; Nucleotide-binding; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..692
FT                   /note="Formate hydrogenlyase transcriptional activator"
FT                   /id="PRO_0000406081"
FT   DOMAIN          202..344
FT                   /note="GAF"
FT   DOMAIN          381..610
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        663..682
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         409..416
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         472..481
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   CONFLICT        639
FT                   /note="A -> D (in Ref. 2; AAA80577)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        691..692
FT                   /note="LA -> FERVAGSDRLEMMRAVAWRFPFTSCADIFPAIPERALIVDKVPHGQ
FT                   DRMPPKHSRTCPTHNLLHLLAFSRLIAVHRALLTAGFSSPNTHLSRRFCA (in Ref.
FT                   2; AAA80577)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   692 AA;  78253 MW;  DD7E43240B2BBE6D CRC64;
     MSYTPMSDLG QQGLFDITRT LLQQPDLASL SEALSQLVKR SALADSAGIV LWQAQSQRAQ
     YYATRENGRP VEYEDETVLA HGPVRRILSR PDALHCNFHE FTETWPQLAA SGLYPEFGHY
     CLLPLAAEGR IFGGCEFIRQ EDRPWSEKEY DRLHTFTQIV GVVAEQIQNR VNNNVDYDLL
     CRERDNFRIL VAITNAVLSR LDIDELVSEV AKEIHHYFNI DAISIVLRSH RKNKLNIYST
     HYLDEHHPAH EQSEVDEAGT LTERVFKSKE MLLINLNERD PLAPYERMLF DTWGNQIQTL
     CLLPLMSGKT MLGVLKLAQC EEKVFTTANL KLLRQIAERV AIAVDNALAY QEIHRLKERL
     VDENLALTEQ LNNVDSEFGE IIGRSEAMYN VLKQVEMVAQ SDSTVLILGE TGTGKELIAR
     AIHNLSGRSG RRMVKMNCAA MPAGLLESDL FGHERGAFTG ASAQRIGRFE LADKSSLFLD
     EVGDMPLELQ PKLLRVLQEQ EFERLGSNKL IQTDVRLIAA TNRDLKKMVA DREFRNDLYY
     RLNVFPIQLP PLRERPEDIP LLVKAFTFKI ARRMGRNIDS IPAETLRTLS SMEWPGNVRE
     LENVVERAVL LTRGNVLQLS LPDITAVTPD TSPVATESAK EGEDEYQLII RVLKETNGVV
     AGPKGAAQRL GLKRTTLLSR MKRLGIDKDA LA