ID   FHLA_SALTY              Reviewed;         692 AA.
AC   P0CL46; P40734; Q9L6U9;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Formate hydrogenlyase transcriptional activator;
GN   Name=fhlA; OrderedLocusNames=STM2859;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DNA-BINDING.
RC   STRAIN=TL 117;
RX   PubMed=10848985; DOI=10.1046/j.1432-1327.2000.01399.x;
RA   Leonhartsberger S., Ehrenreich A., Bock A.;
RT   "Analysis of the domain structure and the DNA binding site of the
RT   transcriptional activator FhlA.";
RL   Eur. J. Biochem. 267:3672-3684(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Required for induction of expression of the formate
CC       dehydrogenase H and hydrogenase-3 structural genes. {ECO:0000250}.
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DR   EMBL; AF232733; AAF70634.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL21739.1; -; Genomic_DNA.
DR   RefSeq; NP_461780.1; NC_003197.2.
DR   RefSeq; WP_000122693.1; NC_003197.2.
DR   AlphaFoldDB; P0CL46; -.
DR   SMR; P0CL46; -.
DR   STRING; 99287.STM2859; -.
DR   PaxDb; P0CL46; -.
DR   EnsemblBacteria; AAL21739; AAL21739; STM2859.
DR   GeneID; 1254382; -.
DR   KEGG; stm:STM2859; -.
DR   PATRIC; fig|99287.12.peg.3015; -.
DR   HOGENOM; CLU_000445_95_0_6; -.
DR   PhylomeDB; P0CL46; -.
DR   BioCyc; SENT99287:STM2859-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   1: Evidence at protein level;
KW   Activator; ATP-binding; DNA-binding; Nucleotide-binding;
KW   Reference proteome; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..692
FT                   /note="Formate hydrogenlyase transcriptional activator"
FT                   /id="PRO_0000081298"
FT   DOMAIN          202..344
FT                   /note="GAF"
FT   DOMAIN          381..610
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        663..682
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         409..416
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         472..481
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   CONFLICT        639
FT                   /note="D -> A (in Ref. 1; AAF70634)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   692 AA;  78297 MW;  787B43240B2D63DB CRC64;
     MSYTPMSDLG QQGLFDITRT LLQQPDLASL SEALSQLVKR SALADSAGIV LWQAQSQRAQ
     YYATRENGRP VEYEDETVLA HGPVRRILSR PDALHCNFHE FTETWPQLAA SGLYPEFGHY
     CLLPLAAEGR IFGGCEFIRQ EDRPWSEKEY DRLHTFTQIV GVVAEQIQNR VNNNVDYDLL
     CRERDNFRIL VAITNAVLSR LDIDELVSEV AKEIHHYFNI DAISIVLRSH RKNKLNIYST
     HYLDEHHPAH EQSEVDEAGT LTERVFKSKE MLLINLNERD PLAPYERMLF DTWGNQIQTL
     CLLPLMSGKT MLGVLKLAQC EEKVFTTANL KLLRQIAERV AIAVDNALAY QEIHRLKERL
     VDENLALTEQ LNNVDSEFGE IIGRSEAMYN VLKQVEMVAQ SDSTVLILGE TGTGKELIAR
     AIHNLSGRSG RRMVKMNCAA MPAGLLESDL FGHERGAFTG ASAQRIGRFE LADKSSLFLD
     EVGDMPLELQ PKLLRVLQEQ EFERLGSNKL IQTDVRLIAA TNRDLKKMVA DREFRNDLYY
     RLNVFPIQLP PLRERPEDIP LLVKAFTFKI ARRMGRNIDS IPAETLRTLS SMEWPGNVRE
     LENVVERAVL LTRGNVLQLS LPDITAVTPD TSPVATESDK EGEDEYQLII RVLKETNGVV
     AGPKGAAQRL GLKRTTLLSR MKRLGIDKDA LA