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FHMAD_MYCTU
ID   FHMAD_MYCTU             Reviewed;         360 AA.
AC   P96809; F2GLS9; I6Y6Z3; L0T5M8;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=F420-dependent hydroxymycolic acid dehydrogenase {ECO:0000303|PubMed:24349169};
DE            Short=fHMAD {ECO:0000303|PubMed:24349169};
DE            EC=1.1.98.- {ECO:0000269|PubMed:24349169};
DE   AltName: Full=FGD2 {ECO:0000303|PubMed:23110042};
DE   Flags: Precursor;
GN   Name=fgd2 {ECO:0000312|EMBL:CCP42857.1};
GN   OrderedLocusNames=Rv0132c {ECO:0000312|EMBL:CCP42857.1},
GN   LH57_00740 {ECO:0000312|EMBL:AIR12853.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   ACTIVITY REGULATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=10879539; DOI=10.1038/35016103;
RA   Stover C.K., Warrener P., VanDevanter D.R., Sherman D.R., Arain T.M.,
RA   Langhorne M.H., Anderson S.W., Towell J.A., Yuan Y., McMurray D.N.,
RA   Kreiswirth B.N., Barry C.E., Baker W.R.;
RT   "A small-molecule nitroimidazopyran drug candidate for the treatment of
RT   tuberculosis.";
RL   Nature 405:962-966(2000).
RN   [4]
RP   SUBSTRATE FOR THE TAT PATHWAY, F420-BINDING, SUBCELLULAR LOCATION, AND
RP   SUBUNIT.
RC   STRAIN=H37Rv;
RX   PubMed=23110042; DOI=10.1371/journal.pone.0045003;
RA   Bashiri G., Perkowski E.F., Turner A.P., Feltcher M.E., Braunstein M.,
RA   Baker E.N.;
RT   "Tat-dependent translocation of an F420-binding protein of Mycobacterium
RT   tuberculosis.";
RL   PLoS ONE 7:E45003-E45003(2012).
RN   [5]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, PATHWAY, AND DRUG
RP   TARGET.
RX   PubMed=24349169; DOI=10.1371/journal.pone.0081985;
RA   Purwantini E., Mukhopadhyay B.;
RT   "Rv0132c of Mycobacterium tuberculosis encodes a coenzyme F420-dependent
RT   hydroxymycolic acid dehydrogenase.";
RL   PLoS ONE 8:E81985-E81985(2013).
CC   -!- FUNCTION: Catalyzes the coenzyme F420-dependent oxidation of
CC       hydroxymycolic acids (H-MAs) to ketomycolic acids (K-MAs), a lipid
CC       class making up the mycobacterial pseudo-outer membrane and over one-
CC       third of the dry weight of M.tuberculosis (PubMed:24349169). Does not
CC       exhibit F420-dependent glucose-6-phosphate dehydrogenase (FGD) activity
CC       (PubMed:23110042). {ECO:0000269|PubMed:23110042,
CC       ECO:0000269|PubMed:24349169}.
CC   -!- ACTIVITY REGULATION: Is inhibited by the anti-tuberculous drug PA-824,
CC       a bicyclic 4-nitroimidazole class compound (PubMed:24349169).
CC       Therefore, this is consistent with the finding that PA-824 inhibits the
CC       formation of K-MAs and causes an accumulation of hydroxymycolic acids
CC       (H-MAs) in M.tuberculosis (PubMed:10879539).
CC       {ECO:0000269|PubMed:10879539, ECO:0000269|PubMed:24349169}.
CC   -!- PATHWAY: Lipid metabolism; mycolic acid biosynthesis.
CC       {ECO:0000269|PubMed:24349169}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:23110042}.
CC   -!- SUBCELLULAR LOCATION: Cell envelope {ECO:0000269|PubMed:23110042}.
CC       Note=The Rv0132c-F420 complex is translocated via the Tat pathway
CC       across the cytoplasmic membrane, where Rv0132c is anchored to the cell
CC       envelope. {ECO:0000269|PubMed:23110042}.
CC   -!- PTM: Is exported by the Tat system. The position of the signal peptide
CC       cleavage has not been experimentally proven.
CC       {ECO:0000269|PubMed:23110042}.
CC   -!- PTM: May be lipidated. {ECO:0000305|PubMed:23110042}.
CC   -!- SIMILARITY: Belongs to the F420-dependent hydroxymycolic acid
CC       dehydrogenase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP42857.1; -; Genomic_DNA.
DR   EMBL; CP009480; AIR12853.1; -; Genomic_DNA.
DR   RefSeq; NP_214646.1; NC_000962.3.
DR   RefSeq; WP_003899826.1; NZ_NVQJ01000001.1.
DR   AlphaFoldDB; P96809; -.
DR   SMR; P96809; -.
DR   STRING; 83332.Rv0132c; -.
DR   PaxDb; P96809; -.
DR   DNASU; 886877; -.
DR   GeneID; 886877; -.
DR   KEGG; mtu:Rv0132c; -.
DR   PATRIC; fig|83332.111.peg.153; -.
DR   TubercuList; Rv0132c; -.
DR   eggNOG; COG2141; Bacteria.
DR   HOGENOM; CLU_027853_4_0_11; -.
DR   OMA; HPWLDNQ; -.
DR   PhylomeDB; P96809; -.
DR   UniPathway; UPA00915; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0031975; C:envelope; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR031017; F420_FGD2.
DR   InterPro; IPR019945; F420_G6P_DH-rel.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR04465; ArgArg_F420; 1.
DR   TIGRFAMs; TIGR03557; F420_G6P_family; 1.
PE   1: Evidence at protein level;
KW   Lipid biosynthesis; Lipid metabolism; Oxidoreductase; Reference proteome;
KW   Signal.
FT   SIGNAL          1..40
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00648"
FT   CHAIN           41..360
FT                   /note="F420-dependent hydroxymycolic acid dehydrogenase"
FT                   /id="PRO_0000435125"
FT   ACT_SITE        78
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
FT   ACT_SITE        147
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
FT   BINDING         77
FT                   /ligand="coenzyme F420-(gamma-Glu)n"
FT                   /ligand_id="ChEBI:CHEBI:133980"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
FT   BINDING         145..146
FT                   /ligand="coenzyme F420-(gamma-Glu)n"
FT                   /ligand_id="ChEBI:CHEBI:133980"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
FT   BINDING         150
FT                   /ligand="coenzyme F420-(gamma-Glu)n"
FT                   /ligand_id="ChEBI:CHEBI:133980"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
FT   BINDING         213..214
FT                   /ligand="coenzyme F420-(gamma-Glu)n"
FT                   /ligand_id="ChEBI:CHEBI:133980"
FT                   /evidence="ECO:0000250|UniProtKB:P9WNE1"
SQ   SEQUENCE   360 AA;  38445 MW;  C42D1911CB57244A CRC64;
     MTGISRRTFG LAAGFGAIGA GGLGGGCSTR SGPTPTPEPA SRGVGVVLSH EQFRTDRLVA
     HAQAAEQAGF RYVWASDHLQ PWQDNEGHSM FPWLTLALVG NSTSSILFGT GVTCPIYRYH
     PATVAQAFAS LAILNPGRVF LGLGTGERLN EQAATDTFGN YRERHDRLIE AIVLIRQLWS
     GERISFTGHY FRTDELKLYD TPAMPPPIFV AASGPQSATL AGRYGDGWIA QARDINDAKL
     LAAFAAGAQA AGRDPTTLGK RAELFAVVGD DKAAARAADL WRFTAGAVDQ PNPVEIQRAA
     ESNPIEKVLA NWAVGTDPGV HIGAVQAVLD AGAVPFLHFP QDDPITAIDF YRTNVLPELR
 
 
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