FHUB_ECOLI
ID FHUB_ECOLI Reviewed; 660 AA.
AC P06972; P77372;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Iron(3+)-hydroxamate import system permease protein FhuB {ECO:0000305};
DE AltName: Full=Ferric hydroxamate uptake protein B {ECO:0000305};
DE AltName: Full=Ferrichrome transport system permease protein FhuB {ECO:0000305};
DE AltName: Full=Ferrichrome uptake protein FhuB {ECO:0000305};
DE AltName: Full=Iron(III)-hydroxamate import system permease protein FhuB {ECO:0000305};
GN Name=fhuB; OrderedLocusNames=b0153, JW0149;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=3020380; DOI=10.1007/bf00331021;
RA Koester W., Braun V.;
RT "Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the
RT fhuB gene and identification of the protein.";
RL Mol. Gen. Genet. 204:435-442(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 311
RP AND 364.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
RX PubMed=2823072; DOI=10.1007/bf00329835;
RA Burkhardt R., Braun V.;
RT "Nucleotide sequence of the fhuC and fhuD genes involved in iron (III)
RT hydroxamate transport: domains in FhuC homologous to ATP-binding
RT proteins.";
RL Mol. Gen. Genet. 209:49-55(1987).
RN [7]
RP FUNCTION IN IRON(3+)-HYDROXAMATE TRANSPORT, AND INTERACTION WITH FHUC.
RC STRAIN=K12;
RX PubMed=1551849; DOI=10.1128/jb.174.7.2305-2311.1992;
RA Schultz-Hauser G., Koester W., Schwarz H., Braun V.;
RT "Iron(III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated
RT membrane association of the FhuC protein and negative complementation of
RT fhuC mutants.";
RL J. Bacteriol. 174:2305-2311(1992).
RN [8]
RP INTERACTION WITH FHUD.
RX PubMed=8522527; DOI=10.1128/jb.177.24.7186-7193.1995;
RA Rohrbach M.R., Braun V., Koester W.;
RT "Ferrichrome transport in Escherichia coli K-12: altered substrate
RT specificity of mutated periplasmic FhuD and interaction of FhuD with the
RT integral membrane protein FhuB.";
RL J. Bacteriol. 177:7186-7193(1995).
RN [9]
RP INTERACTION WITH FHUD, AND DOMAIN.
RX PubMed=9426146; DOI=10.1046/j.1365-2958.1997.6592008.x;
RA Mademidis A., Killmann H., Kraas W., Flechsler I., Jung G., Braun V.;
RT "ATP-dependent ferric hydroxamate transport system in Escherichia coli:
RT periplasmic FhuD interacts with a periplasmic and with a
RT transmembrane/cytoplasmic region of the integral membrane protein FhuB, as
RT revealed by competitive peptide mapping.";
RL Mol. Microbiol. 26:1109-1123(1997).
RN [10]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [11]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Part of the ABC transporter complex FhuCDB involved in
CC iron(3+)-hydroxamate import. Responsible for the translocation of the
CC substrate across the membrane. {ECO:0000269|PubMed:1551849,
CC ECO:0000269|PubMed:3020380}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (FhuC),
CC two transmembrane proteins (FhuB) and a solute-binding protein (FhuD)
CC (Probable). FhuB interacts with FhuC and FhuD (PubMed:1551849,
CC PubMed:8522527, PubMed:9426146). {ECO:0000269|PubMed:1551849,
CC ECO:0000269|PubMed:8522527, ECO:0000269|PubMed:9426146, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255, ECO:0000269|PubMed:15919996}.
CC -!- INDUCTION: Induced 2.1-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- DOMAIN: FhuD interacts with a periplasmic and a
CC transmembrane/cytoplasmic region of FhuB. The transmembrane region may
CC be part of a pore through which a portion of FhuD inserts into the
CC cytoplasmic membrane during transport. {ECO:0000269|PubMed:9426146}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. FecCD subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA27853.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X04319; CAA27852.1; -; Genomic_DNA.
DR EMBL; X04319; CAA27853.1; ALT_INIT; Genomic_DNA.
DR EMBL; U70214; AAB08583.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73264.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96729.2; -; Genomic_DNA.
DR EMBL; X05810; CAA29256.1; -; Genomic_DNA.
DR PIR; A64739; A64739.
DR RefSeq; NP_414695.1; NC_000913.3.
DR RefSeq; WP_000044072.1; NZ_LN832404.1.
DR PDB; 7LB8; EM; 3.40 A; B=1-660.
DR PDBsum; 7LB8; -.
DR AlphaFoldDB; P06972; -.
DR SMR; P06972; -.
DR BioGRID; 4259735; 165.
DR ComplexPortal; CPX-4286; Ferric-hydroxamate ABC transporter complex.
DR DIP; DIP-9603N; -.
DR IntAct; P06972; 1.
DR STRING; 511145.b0153; -.
DR TCDB; 3.A.1.14.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P06972; -.
DR PRIDE; P06972; -.
DR EnsemblBacteria; AAC73264; AAC73264; b0153.
DR EnsemblBacteria; BAB96729; BAB96729; BAB96729.
DR GeneID; 946890; -.
DR KEGG; ecj:JW0149; -.
DR KEGG; eco:b0153; -.
DR PATRIC; fig|1411691.4.peg.2127; -.
DR EchoBASE; EB0299; -.
DR eggNOG; COG0609; Bacteria.
DR HOGENOM; CLU_013016_7_3_6; -.
DR InParanoid; P06972; -.
DR OMA; MFPYQIP; -.
DR PhylomeDB; P06972; -.
DR BioCyc; EcoCyc:FHUB-MON; -.
DR BioCyc; MetaCyc:FHUB-MON; -.
DR PRO; PR:P06972; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; TAS:EcoCyc.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0098711; P:iron ion import across plasma membrane; IC:ComplexPortal.
DR GO; GO:0033214; P:siderophore-dependent iron import into cell; IBA:GO_Central.
DR Gene3D; 1.10.3470.10; -; 2.
DR InterPro; IPR037294; ABC_BtuC-like.
DR InterPro; IPR000522; ABC_transptr_permease_BtuC.
DR PANTHER; PTHR30472; PTHR30472; 2.
DR Pfam; PF01032; FecCD; 2.
DR SUPFAM; SSF81345; SSF81345; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Ion transport; Iron;
KW Iron transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..660
FT /note="Iron(3+)-hydroxamate import system permease protein
FT FhuB"
FT /id="PRO_0000060028"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 567..587
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 607..627
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 635..655
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 11
FT /note="L -> V (in Ref. 6; CAA29256)"
FT /evidence="ECO:0000305"
FT CONFLICT 311
FT /note="T -> I (in Ref. 1; CAA27852/CAA27853)"
FT /evidence="ECO:0000305"
FT CONFLICT 364
FT /note="Missing (in Ref. 1; CAA27852/CAA27853)"
FT /evidence="ECO:0000305"
FT HELIX 7..31
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 37..41
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 50..53
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 55..82
FT /evidence="ECO:0007829|PDB:7LB8"
FT TURN 90..94
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 95..107
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 115..133
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 143..167
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 173..178
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 186..188
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 189..206
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 209..216
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 219..224
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 229..250
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 257..259
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 261..268
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 273..301
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 308..322
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 349..369
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 372..375
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 381..386
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 387..389
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 391..415
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 422..425
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 427..441
FT /evidence="ECO:0007829|PDB:7LB8"
FT TURN 446..448
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 450..452
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 455..472
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 476..499
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 505..512
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 521..537
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 538..541
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 542..547
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 553..558
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 562..583
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 589..601
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 606..630
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 632..635
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 639..657
FT /evidence="ECO:0007829|PDB:7LB8"
SQ SEQUENCE 660 AA; 70423 MW; 427313E7B421B0BC CRC64;
MSKRIALFPA LLLALLVIVA TALTWMNFSQ ALPRSQWAQA AWSPDIDVIE QMIFHYSLLP
RLAISLLVGA GLGLVGVLFQ QVLRNPLAEP TTLGVATGAQ LGITVTTLWA IPGAMASQFA
AQAGACVVGL IVFGVAWGKR LSPVTLILAG LVVSLYCGAI NQLLVIFHHD QLQSMFLWST
GTLTQTDWGG VERLWPQLLG GVMLTLLLLR PLTLMGLDDG VARNLGLALS LARLAALSLA
IVISALLVNA VGIIGFIGLF APLLAKMLGA RRLLPRLMLA SLIGALILWL SDQIILWLTR
VWMEVSTGSV TALIGAPLLL WLLPRLRSIS APDMKVNDRV AAERQHVLAF ALAGGVLLLM
AVVVALSFGR DAHGWTWASG ALLEDLMPWR WPRIMAALFA GVMLAVAGCI IQRLTGNPMA
SPEVLGISSG AAFGVVLMLF LVPGNAFGWL LPAGSLGAAV TLLIIMIAAG RGGFSPHRML
LAGMALSTAF TMLLMMLQAS GDPRMAQVLT WISGSTYNAT DAQVWRTGIV MVILLAITPL
CRRWLTILPL GGDTARAVGM ALTPTRIALL LLAACLTATA TMTIGPLSFV GLMAPHIARM
MGFRRTMPHI VISALVGGLL LVFADWCGRM VLFPFQIPAG LLSTFIGAPY FIYLLRKQSR
