FHUC_ECOLI
ID FHUC_ECOLI Reviewed; 265 AA.
AC P07821; P77548;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Iron(3+)-hydroxamate import ATP-binding protein FhuC {ECO:0000305};
DE EC=7.2.2.16 {ECO:0000269|PubMed:1551849};
DE AltName: Full=Ferric hydroxamate uptake protein C {ECO:0000305};
DE AltName: Full=Ferrichrome transport ATP-binding protein FhuC {ECO:0000305};
DE AltName: Full=Iron(III)-hydroxamate import ATP-binding protein FhuC {ECO:0000305};
GN Name=fhuC; OrderedLocusNames=b0151, JW0147;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3301821; DOI=10.1128/jb.169.8.3844-3849.1987;
RA Coulton J.W., Mason P., Allatt D.D.;
RT "fhuC and fhuD genes for iron (III)-ferrichrome transport into Escherichia
RT coli K-12.";
RL J. Bacteriol. 169:3844-3849(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2823072; DOI=10.1007/bf00329835;
RA Burkhardt R., Braun V.;
RT "Nucleotide sequence of the fhuC and fhuD genes involved in iron (III)
RT hydroxamate transport: domains in FhuC homologous to ATP-binding
RT proteins.";
RL Mol. Gen. Genet. 209:49-55(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [7]
RP FUNCTION IN IRON(3+)-HYDROXAMATE TRANSPORT, CATALYTIC ACTIVITY, INTERACTION
RP WITH FHUB, SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-50 AND ASP-172.
RC STRAIN=K12;
RX PubMed=1551849; DOI=10.1128/jb.174.7.2305-2311.1992;
RA Schultz-Hauser G., Koester W., Schwarz H., Braun V.;
RT "Iron(III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated
RT membrane association of the FhuC protein and negative complementation of
RT fhuC mutants.";
RL J. Bacteriol. 174:2305-2311(1992).
RN [8]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Part of the ABC transporter complex FhuCDB involved in
CC iron(3+)-hydroxamate import. Responsible for energy coupling to the
CC transport system. {ECO:0000269|PubMed:1551849}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Fe(3+)-hydroxamate complex-[hydroxamate-binding
CC protein]Side 1 = ADP + phosphate + Fe(3+)-hydroxamate complexSide 2 +
CC [hydroxamate-binding protein]Side 1.; EC=7.2.2.16;
CC Evidence={ECO:0000269|PubMed:1551849};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (FhuC),
CC two transmembrane proteins (FhuB) and a solute-binding protein (FhuD)
CC (Probable). FhuC interacts with FhuB (PubMed:1551849).
CC {ECO:0000269|PubMed:1551849, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:1551849};
CC Peripheral membrane protein {ECO:0000269|PubMed:1551849}. Note=FhuB
CC mediates the association of FhuC with the cytoplasmic membrane.
CC {ECO:0000269|PubMed:1551849}.
CC -!- INDUCTION: Induced 1.6-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Iron (Fe3+)-
CC hydroxamate importer (TC 3.A.1.14.7) family. {ECO:0000305}.
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DR EMBL; M12486; AAB61769.1; -; Genomic_DNA.
DR EMBL; X05810; CAA29254.1; -; Genomic_DNA.
DR EMBL; U70214; AAB08581.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73262.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96727.1; -; Genomic_DNA.
DR PIR; G64738; QRECFH.
DR RefSeq; NP_414693.1; NC_000913.3.
DR RefSeq; WP_001158931.1; NZ_STEB01000032.1.
DR PDB; 7LB8; EM; 3.40 A; C/U=1-265.
DR PDBsum; 7LB8; -.
DR AlphaFoldDB; P07821; -.
DR SMR; P07821; -.
DR BioGRID; 4261072; 353.
DR BioGRID; 849632; 2.
DR ComplexPortal; CPX-4286; Ferric-hydroxamate ABC transporter complex.
DR IntAct; P07821; 5.
DR STRING; 511145.b0151; -.
DR TCDB; 3.A.1.14.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P07821; -.
DR PRIDE; P07821; -.
DR EnsemblBacteria; AAC73262; AAC73262; b0151.
DR EnsemblBacteria; BAB96727; BAB96727; BAB96727.
DR GeneID; 945250; -.
DR KEGG; ecj:JW0147; -.
DR KEGG; eco:b0151; -.
DR PATRIC; fig|1411691.4.peg.2129; -.
DR EchoBASE; EB0300; -.
DR eggNOG; COG1120; Bacteria.
DR InParanoid; P07821; -.
DR OMA; RYAWNGL; -.
DR PhylomeDB; P07821; -.
DR BioCyc; EcoCyc:FHUC-MON; -.
DR BioCyc; MetaCyc:FHUC-MON; -.
DR PRO; PR:P07821; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; TAS:EcoCyc.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0015625; F:ABC-type ferric hydroxamate transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR GO; GO:0098711; P:iron ion import across plasma membrane; IC:ComplexPortal.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell inner membrane; Cell membrane;
KW Ion transport; Iron; Iron transport; Membrane; Nucleotide-binding;
KW Reference proteome; Translocase; Transport.
FT CHAIN 1..265
FT /note="Iron(3+)-hydroxamate import ATP-binding protein
FT FhuC"
FT /id="PRO_0000092327"
FT DOMAIN 12..248
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 44..51
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 168..179
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT MUTAGEN 50
FT /note="K->Q: Lack of activity."
FT /evidence="ECO:0000269|PubMed:1551849"
FT MUTAGEN 172
FT /note="D->E: Lack of activity."
FT /evidence="ECO:0000269|PubMed:1551849"
FT CONFLICT 31
FT /note="S -> C (in Ref. 2; CAA29254)"
FT /evidence="ECO:0000305"
FT CONFLICT 226..249
FT /note="MIAQGTPAEIMRGETLEMIYGIPM -> SDCSGNACGNYARRNPRNDLWHPD
FT (in Ref. 1; AAB61769 and 3; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT STRAND 12..17
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 36..43
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 50..57
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 80..86
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 102..108
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 111..114
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 125..134
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 139..141
FT /evidence="ECO:0007829|PDB:7LB8"
FT TURN 145..147
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 150..163
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 167..175
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 180..197
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 200..204
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 208..213
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 216..220
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 228..230
FT /evidence="ECO:0007829|PDB:7LB8"
FT TURN 232..234
FT /evidence="ECO:0007829|PDB:7LB8"
FT HELIX 237..245
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 255..259
FT /evidence="ECO:0007829|PDB:7LB8"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:7LB8"
SQ SEQUENCE 265 AA; 28886 MW; 2511770D6D9EE772 CRC64;
MQEYTNHSDT TFALRNISFR VPGRTLLHPL SLTFPAGKVT GLIGHNGSGK STLLKMLGRH
QPPSEGEILL DAQPLESWSS KAFARKVAYL PQQLPPAEGM TVRELVAIGR YPWHGALGRF
GAADREKVEE AISLVGLKPL AHRLVDSLSG GERQRAWIAM LVAQDSRCLL LDEPTSALDI
AHQVDVLSLV HRLSQERGLT VIAVLHDINM AARYCDYLVA LRGGEMIAQG TPAEIMRGET
LEMIYGIPMG ILPHPAGAAP VSFVY
