FHUE_ECOLI
ID FHUE_ECOLI Reviewed; 729 AA.
AC P16869; P77292;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=FhuE receptor;
DE AltName: Full=Outer-membrane receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B and Fe(III)-rhodotrulic acid;
DE Flags: Precursor;
GN Name=fhuE; OrderedLocusNames=b1102, JW1088;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF VAL-44; VAL-46 AND
RP GLY-163.
RC STRAIN=K12;
RX PubMed=2162465; DOI=10.1111/j.1365-2958.1990.tb00609.x;
RA Sauer U., Hantke K., Braun V.;
RT "Sequence of the fhuE outer-membrane receptor gene of Escherichia coli K12
RT and properties of mutants.";
RL Mol. Microbiol. 4:427-437(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF 37-50.
RC STRAIN=K12;
RX PubMed=3032906; DOI=10.1128/jb.169.5.2044-2049.1987;
RA Sauer M., Hantke K., Braun V.;
RT "Ferric-coprogen receptor FhuE of Escherichia coli: processing and sequence
RT common to all TonB-dependent outer membrane receptor proteins.";
RL J. Bacteriol. 169:2044-2049(1987).
RN [6]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Required for the uptake of Fe(3+) via coprogen, ferrioxamine
CC B, and rhodotorulic acid.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC -!- INDUCTION: For induction, the TonB and the ExbB proteins have to be
CC active. Induced by hydroxyurea (PubMed:20005847).
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC {ECO:0000305}.
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DR EMBL; X17615; CAA35616.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74186.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35909.1; -; Genomic_DNA.
DR PIR; C64854; C64854.
DR RefSeq; NP_415620.1; NC_000913.3.
DR RefSeq; WP_000953441.1; NZ_SSZK01000019.1.
DR PDB; 6E4V; X-ray; 2.00 A; A=37-729.
DR PDBsum; 6E4V; -.
DR AlphaFoldDB; P16869; -.
DR SMR; P16869; -.
DR BioGRID; 4260942; 181.
DR ComplexPortal; CPX-3579; Ferric-coprogen outer membrane transporter complex.
DR STRING; 511145.b1102; -.
DR TCDB; 1.B.14.1.1; the outer membrane receptor (omr) family.
DR PaxDb; P16869; -.
DR PRIDE; P16869; -.
DR EnsemblBacteria; AAC74186; AAC74186; b1102.
DR EnsemblBacteria; BAA35909; BAA35909; BAA35909.
DR GeneID; 945649; -.
DR KEGG; ecj:JW1088; -.
DR KEGG; eco:b1102; -.
DR PATRIC; fig|1411691.4.peg.1166; -.
DR EchoBASE; EB0302; -.
DR eggNOG; COG4773; Bacteria.
DR HOGENOM; CLU_008287_9_3_6; -.
DR InParanoid; P16869; -.
DR OMA; GRLDWYD; -.
DR PhylomeDB; P16869; -.
DR BioCyc; EcoCyc:EG10306-MON; -.
DR BioCyc; MetaCyc:EG10306-MON; -.
DR PRO; PR:P16869; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR GO; GO:0055072; P:iron ion homeostasis; IC:ComplexPortal.
DR Gene3D; 2.170.130.10; -; 1.
DR Gene3D; 2.40.170.20; -; 1.
DR InterPro; IPR012910; Plug_dom.
DR InterPro; IPR037066; Plug_dom_sf.
DR InterPro; IPR039423; TonB-dep_rcpt.
DR InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR InterPro; IPR010916; TonB_box_CS.
DR InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR InterPro; IPR010917; TonB_rcpt_CS.
DR InterPro; IPR010105; TonB_sidphr_rcpt.
DR PANTHER; PTHR32552; PTHR32552; 1.
DR Pfam; PF07715; Plug; 1.
DR Pfam; PF00593; TonB_dep_Rec; 1.
DR TIGRFAMs; TIGR01783; TonB-siderophor; 1.
DR PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Direct protein sequencing;
KW Ion transport; Iron; Iron transport; Membrane; Receptor;
KW Reference proteome; Signal; TonB box; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..36
FT /evidence="ECO:0000269|PubMed:3032906"
FT CHAIN 37..729
FT /note="FhuE receptor"
FT /id="PRO_0000034749"
FT REGION 48..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 42..49
FT /note="TonB box"
FT MOTIF 712..729
FT /note="TonB C-terminal box"
FT COMPBIAS 56..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 44
FT /note="V->P: Abolishes transport activity."
FT /evidence="ECO:0000269|PubMed:2162465"
FT MUTAGEN 46
FT /note="V->P: Abolishes transport activity."
FT /evidence="ECO:0000269|PubMed:2162465"
FT MUTAGEN 163
FT /note="G->A,V,N,D,H: Impaired transport and growth."
FT /evidence="ECO:0000269|PubMed:2162465"
FT CONFLICT 363
FT /note="G -> C (in Ref. 1; CAA35616)"
FT /evidence="ECO:0000305"
FT TURN 67..69
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 79..85
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 87..93
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 98..103
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 109..114
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 135..138
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 147..149
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 155..163
FT /evidence="ECO:0007829|PDB:6E4V"
FT TURN 166..168
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 174..182
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 191..199
FT /evidence="ECO:0007829|PDB:6E4V"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 203..215
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 220..232
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 239..253
FT /evidence="ECO:0007829|PDB:6E4V"
FT TURN 254..256
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 257..272
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 302..319
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 322..342
FT /evidence="ECO:0007829|PDB:6E4V"
FT TURN 348..350
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 358..360
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 362..364
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 368..392
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 395..419
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 421..424
FT /evidence="ECO:0007829|PDB:6E4V"
FT HELIX 427..429
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 443..464
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 467..481
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 486..501
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 503..517
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 532..545
FT /evidence="ECO:0007829|PDB:6E4V"
FT TURN 546..549
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 550..571
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 575..577
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 579..584
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 588..601
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 604..616
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 622..624
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 629..639
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 646..655
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 658..664
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 667..673
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 676..686
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 688..699
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 711..713
FT /evidence="ECO:0007829|PDB:6E4V"
FT STRAND 720..728
FT /evidence="ECO:0007829|PDB:6E4V"
SQ SEQUENCE 729 AA; 81233 MW; 68ACEE7D110F76CD CRC64;
MLSTQFNRDN QYQAITKPSL LAGCIALALL PSAAFAAPAT EETVIVEGSA TAPDDGENDY
SVTSTSAGTK MQMTQRDIPQ SVTIVSQQRM EDQQLQTLGE VMENTLGISK SQADSDRALY
YSRGFQIDNY MVDGIPTYFE SRWNLGDALS DMALFERVEV VRGATGLMTG TGNPSAAINM
VRKHATSREF KGDVSAEYGS WNKERYVADL QSPLTEDGKI RARIVGGYQN NDSWLDRYNS
EKTFFSGIVD ADLGDLTTLS AGYEYQRIDV NSPTWGGLPR WNTDGSSNSY DRARSTAPDW
AYNDKEINKV FMTLKQQFAD TWQATLNATH SEVEFDSKMM YVDAYVNKAD GMLVGPYSNY
GPGFDYVGGT GWNSGKRKVD ALDLFADGSY ELFGRQHNLM FGGSYSKQNN RYFSSWANIF
PDEIGSFYNF NGNFPQTDWS PQSLAQDDTT HMKSLYAATR VTLADPLHLI LGARYTNWRV
DTLTYSMEKN HTTPYAGLVF DINDNWSTYA SYTSIFQPQN DRDSSGKYLA PITGNNYELG
LKSDWMNSRL TTTLAIFRIE QDNVAQSTGT PIPGSNGETA YKAVDGTVSK GVEFELNGAI
TDNWQLTFGA TRYIAEDNEG NAVNPNLPRT TVKMFTSYRL PVMPELTVGG GVNWQNRVYT
DTVTPYGTFR AEQGSYALVD LFTRYQVTKN FSLQGNVNNL FDKTYDTNVE GSIVYGTPRN
FSITGTYQF
