FIB12_BPT4
ID FIB12_BPT4 Reviewed; 527 AA.
AC P10930;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 3.
DT 23-FEB-2022, entry version 125.
DE RecName: Full=Short tail fiber protein gp12;
DE AltName: Full=Gene product 12;
DE Short=gp12;
GN Name=12;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D;
RX PubMed=3357780; DOI=10.1093/nar/16.5.2334;
RA Selivanov N.A., Prilipov A.G., Mesyanzhinov V.V.;
RT "Nucleotide and deduced amino acid sequence of bacteriophage T4 gene 12.";
RL Nucleic Acids Res. 16:2334-2334(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
RC STRAIN=D;
RX PubMed=2548819; DOI=10.1089/dna.1.1989.8.287;
RA Barrett B.K., Berget P.B.;
RT "Using transposon Tn5 insertions to sequence bacteriophage T4 gene 11.";
RL DNA 8:287-295(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4.
RC STRAIN=D;
RX PubMed=2726468; DOI=10.1093/nar/17.8.3303;
RA Prilipov A.G., Selivanov N.A., Efimov V.P., Marusich E.I.,
RA Mesyanzhinov V.V.;
RT "Nucleotide sequences of bacteriophage T4 genes 9, 10 and 11.";
RL Nucleic Acids Res. 17:3303-3303(1989).
RN [5]
RP FUNCTION.
RX PubMed=12837775; DOI=10.1128/jb.185.14.4022-4030.2003;
RA Weigele P.R., Scanlon E., King J.;
RT "Homotrimeric, beta-stranded viral adhesins and tail proteins.";
RL J. Bacteriol. 185:4022-4030(2003).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 250-527, AND SUBUNIT.
RX PubMed=11743729; DOI=10.1006/jmbi.2000.5204;
RA van Raaij M.J., Schoehn G., Burda M.R., Miller S.;
RT "Crystal structure of a heat and protease-stable part of the bacteriophage
RT T4 short tail fibre.";
RL J. Mol. Biol. 314:1137-1146(2001).
RN [7]
RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP LOCATION, AND FUNCTION.
RX PubMed=27193680; DOI=10.1038/nature17971;
RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT "Structure of the T4 baseplate and its function in triggering sheath
RT contraction.";
RL Nature 533:346-352(2016).
CC -!- FUNCTION: Structural component of the short tail fiber. Adhesion
CC protein that binds irreversibly to the lipopolysaccharides component
CC (LPS) on the cell surface of Escherichia coli B strains during virus
CC attachment. After at least three long tail fibers have bound, short
CC tail fibers extend and bind irreversibly to the core region of the host
CC cell LPS. {ECO:0000269|PubMed:12837775, ECO:0000269|PubMed:27193680}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:11743729,
CC ECO:0000269|PubMed:27193680}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:27193680}.
CC -!- SIMILARITY: Belongs to the tevenvirinae short tail fiber protein
CC family. {ECO:0000305}.
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DR EMBL; X06792; CAA29951.1; -; Genomic_DNA.
DR EMBL; AF158101; AAD42417.1; -; Genomic_DNA.
DR EMBL; M26253; AAA32495.1; -; Genomic_DNA.
DR EMBL; X14192; CAA32398.1; -; Genomic_DNA.
DR PIR; C32479; C32479.
DR PIR; S01889; GIBPT4.
DR RefSeq; NP_049770.1; NC_000866.4.
DR PDB; 1H6W; X-ray; 1.90 A; A=85-396, B=518-527.
DR PDB; 1OCY; X-ray; 1.50 A; A=332-527.
DR PDB; 1PDI; EM; 12.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=250-527.
DR PDB; 5IV5; EM; 4.11 A; AI/AJ/BA/DB/DC/DD/FE/FF/FG/HH/HI/HJ/O/P/Q/l/m/n=1-527.
DR PDBsum; 1H6W; -.
DR PDBsum; 1OCY; -.
DR PDBsum; 1PDI; -.
DR PDBsum; 5IV5; -.
DR SMR; P10930; -.
DR DrugBank; DB04272; Citric acid.
DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR GeneID; 1258789; -.
DR KEGG; vg:1258789; -.
DR EvolutionaryTrace; P10930; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR GO; GO:0098024; C:virus tail, fiber; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1340.10; -; 1.
DR Gene3D; 4.10.1070.10; -; 1.
DR InterPro; IPR015173; Phage_T4_Gp12.
DR InterPro; IPR011083; Phage_tail_collar_dom.
DR InterPro; IPR037053; Phage_tail_collar_dom_sf.
DR InterPro; IPR027448; Short_tail_fibre_C.
DR InterPro; IPR044916; Short_tail_fibre_C_sf.
DR Pfam; PF07484; Collar; 1.
DR Pfam; PF09089; gp12-short_mid; 1.
DR Pfam; PF14928; S_tail_recep_bd; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host-virus interaction; Late protein; Reference proteome;
KW Viral attachment to host cell; Viral attachment to host entry receptor;
KW Viral tail fiber protein; Viral tail protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..527
FT /note="Short tail fiber protein gp12"
FT /id="PRO_0000165002"
FT CONFLICT 33
FT /note="Q -> H (in Ref. 1; CAA29951 and 2; AAD42417)"
FT /evidence="ECO:0000305"
FT HELIX 247..250
FT /evidence="ECO:0007829|PDB:1H6W"
FT STRAND 257..259
FT /evidence="ECO:0007829|PDB:1H6W"
FT STRAND 264..268
FT /evidence="ECO:0007829|PDB:1H6W"
FT STRAND 276..281
FT /evidence="ECO:0007829|PDB:1H6W"
FT HELIX 335..340
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 346..353
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 359..364
FT /evidence="ECO:0007829|PDB:1OCY"
FT TURN 369..371
FT /evidence="ECO:0007829|PDB:1OCY"
FT HELIX 373..378
FT /evidence="ECO:0007829|PDB:1OCY"
FT TURN 379..383
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 387..391
FT /evidence="ECO:0007829|PDB:1OCY"
FT HELIX 407..410
FT /evidence="ECO:0007829|PDB:1OCY"
FT HELIX 412..414
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 415..417
FT /evidence="ECO:0007829|PDB:1OCY"
FT TURN 425..428
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 454..456
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 465..467
FT /evidence="ECO:0007829|PDB:1OCY"
FT HELIX 493..495
FT /evidence="ECO:0007829|PDB:1OCY"
FT HELIX 498..500
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 508..510
FT /evidence="ECO:0007829|PDB:1OCY"
FT STRAND 520..524
FT /evidence="ECO:0007829|PDB:1OCY"
SQ SEQUENCE 527 AA; 56205 MW; F86C7E65205486CC CRC64;
MSNNTYQHVS NESRYVKFDP TDTNFPPEIT DVQAAIAAIS PAGVNGVPDA SSTTKGILFI
PTEQEVIDGT NNTKAVTPAT LATRLSYPNA TETVYGLTRY STNDEAIAGV NNESSITPAK
FTVALNNAFE TRVSTESSNG VIKISSLPQA LAGADDTTAM TPLKTQQLAI KLIAQIAPSE
TTATESDQGV VQLATVAQVR QGTLREGYAI SPYTFMNSSS TEEYKGVIKL GTQSEVNSNN
ASVAVTGATL NGRGSTTSMR GVVKLTTTAG SQSGGDASSA LAWNADVIQQ RGGQIIYGTL
RIEDTFTIAN GGANITGTVR MTGGYIQGNR IVTQNEIDRT IPVGAIMMWA ADSLPSDAWR
FCHGGTVSAS DCPLYASRIG TRYGGNPSNP GLPDMRGLFV RGSGRGSHLT NPNVNGNDQF
GKPRLGVGCT GGYVGEVQIQ QMSYHKHAGG FGEHDDLGAF GNTRRSNFVG TRKGLDWDNR
SYFTNDGYEI DPESQRNSKY TLNRPELIGN ETRPWNISLN YIIKVKE
