FIBER_BPSPP
ID FIBER_BPSPP Reviewed; 1111 AA.
AC Q0PDK6;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 29-SEP-2021, entry version 47.
DE RecName: Full=Tail spike protein {ECO:0000305};
DE Short=TSP;
DE AltName: Full=Gene product 21;
DE Short=Gp21;
GN Name=21;
OS Bacillus phage SPP1 (Bacteriophage SPP1).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae.
OX NCBI_TaxID=10724;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1] {ECO:0000312|Proteomes:UP000002559}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=9434185; DOI=10.1016/s0378-1119(97)00547-7;
RA Alonso J.C., Luder G., Stiege A.C., Chai S., Weise F., Trautner T.A.;
RT "The complete nucleotide sequence and functional organization of Bacillus
RT subtilis bacteriophage SPP1.";
RL Gene 204:201-212(1997).
RN [2]
RP FUNCTION, DOMAIN, INTERACTION WITH HOST YUEB, AND SUBCELLULAR LOCATION.
RX PubMed=22171743; DOI=10.1111/j.1365-2958.2011.07931.x;
RA Vinga I., Baptista C., Auzat I., Petipas I., Lurz R., Tavares P.,
RA Santos M.A., Sao-Jose C.;
RT "Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor
RT binding and trigger of phage DNA ejection.";
RL Mol. Microbiol. 83:289-303(2012).
CC -!- FUNCTION: Binds to the host entry receptor YueB and triggers viral DNA
CC ejection. Binding to Yueb. This interaction initiates a cascade of
CC conformational changes at the gp21 C-terminus causing disassembly of
CC the tail spike organization and opening of the cap region formed by the
CC N-terminus of gp21. {ECO:0000269|PubMed:22171743}.
CC -!- SUBUNIT: Interacts with host Yueb receptor; this interaction triggers
CC viral DNA ejection. {ECO:0000269|PubMed:22171743}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305|PubMed:22171743}.
CC -!- DOMAIN: The N-terminus is part of the distal tail cap. The C-terminus
CC is part of the spike structure and is probably involved in host
CC receptor binding. {ECO:0000269|PubMed:22171743}.
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DR EMBL; X97918; CAL18688.1; -; Genomic_DNA.
DR RefSeq; YP_009268706.1; NC_004166.2.
DR SMR; Q0PDK6; -.
DR GeneID; 955339; -.
DR KEGG; vg:955339; -.
DR Proteomes; UP000002559; Genome.
DR GO; GO:0098024; C:virus tail, fiber; IDA:UniProtKB.
DR GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IDA:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR007119; Phage_minor_struct_N.
DR TIGRFAMs; TIGR01665; put_anti_recept; 1.
PE 1: Evidence at protein level;
KW Host-virus interaction; Reference proteome; Viral attachment to host cell;
KW Viral attachment to host entry receptor;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT CHAIN 1..1111
FT /note="Tail spike protein"
FT /id="PRO_0000438142"
SQ SEQUENCE 1111 AA; 123953 MW; 6743A97C141BE172 CRC64;
MRSLSKNIWI MSNFEVLLTT LTGDRDAGGC RYWNGSFEEP LSPGETATYQ FYADASHPNS
KYVINDNKVI FKEPEQFGGD YRMFTIIDTE LGRSEQDGRY IIATCEPAWY ELDDDFIENK
RPTDKTPQEA LDLLLEGTRY EGEVDPDLQM RASDSFYQVS VKEAVIQFFQ TWGGYFKDTI
EFDGNEITRR VIKSLKRRGA ANGVRFEHDK DITSIRRNIT GYPKTALYPY GASLEMTDED
GNATGGYTRY IDIKDVVWSK AAGDPVDKPA GQAWIGDPDL LKVFGRPMPD GSLRHRWTRW
QNEDITTPEE LIEAAYYDLI NNVSKAIVNY NVEVAPKNVK LGDTCIILDR EFATPIELEA
DVIKMTYFTD RPEEGATIEL GEYLNLDDIE RQIGAINDRF KKNEGKWNTG GGITEVTDGA
FPDKKPPKPS NITVKSMFQS IFLSWNYDPS SYIAGYRVYA SKTPGFTPTD NDIIFEGKMS
GYEHKVGNNE TWYYRLSTVN YHKTESDMSD QYSAQSERIM NDDILFGAIT ADKLANLAVT
ADKISRSFDE GNIFPGSLLK PSQFYDYPNT KHSVTDKTFN ELTITQVIDD STRLRFGISG
QNKAPNNIQR MPLEQGQIYT LSFEVKRNNT TDLSYIHLKK PDNTFMLLSS SLKDISAYPS
DEFVRVDLQF TAPETSSKFT LGFGGYNRNG NVTPCSFVIR KVQVRKGTEI KDYGYSPYDI
MLVDGAIYSD YIQSAAINSA HIADAAIGSA AIQNAAIKNA HLGTAIVDTA NIKDAAITSA
KIKELSADLI TSGTINAINI TGSLIRGGRF EPLNESSAYT SYIEGDKIYQ RVNTINYGGT
PKKTYNDLTI TSGGVTMVYG GRDTGTDEPL REMTLRDGSL YMSGNKNDTG YSEIQMYCND
DDVVNAPKSI FRQKLGGVNR MSLHSYYNSV PGSYNVQMDT RNTSIYSHFA NGWQLTADGK
IMIMSKNGQG IYLDPSNDNV AGQVTMHVGN AGQAGGLIQP VIAGAPHYSI TDIHGALQTN
MSVMLYQYNF VMPSKGDAYA SKYNDIPPPF ECENIFMVVP TVFGGYSDHV HATITSQSSS
GFRCYVRGTG SSTGILGRTF QVRFLIFYEV N