FIBL_GALME
ID FIBL_GALME Reviewed; 267 AA.
AC Q26427;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Fibroin light chain;
DE Short=Fib-L;
DE AltName: Full=L-fibroin;
DE AltName: Full=PG-1;
DE Contains:
DE RecName: Full=Fibroin light chain, short form;
DE Flags: Precursor;
GN Name=FIBL;
OS Galleria mellonella (Greater wax moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC Pyralidae; Galleriinae; Galleria.
OX NCBI_TaxID=7137;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 17-27.
RC TISSUE=Posterior silk gland;
RX PubMed=7715595; DOI=10.1007/bf00425815;
RA Zurovec M., Vaskova M., Kodrik D., Sehnal F., Kumaran A.K.;
RT "Light-chain fibroin of Galleria mellonella L.";
RL Mol. Gen. Genet. 247:1-6(1995).
CC -!- FUNCTION: It is likely that the major role of L-chain is to prevent the
CC retention of H-chain in ER by forming the disulfide linkage.
CC {ECO:0000250}.
CC -!- SUBUNIT: Silk fibroin elementary unit consists in a disulfide-linked
CC heavy and light chain and a p25 glycoprotein in molar ratios of 6:6:1.
CC This results in a complex of approximately 2.3 MDa.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced exclusively in the posterior (PSG) section
CC of silk glands, which are essentially modified salivary glands.
CC -!- DEVELOPMENTAL STAGE: Expressed in the posterior silk glands throughout
CC the penultimate and last larval instars. Declines in immobile pupae and
CC disappears within the next 12 hours when insects pupate.
CC -!- PTM: Partially N-terminally processed to yield a short form which lacks
CC the first two residues of the long form. {ECO:0000250}.
CC -!- PTM: The interchain disulfide bridge is essential for the intracellular
CC transport and secretion of fibroin. {ECO:0000250}.
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DR EMBL; S77817; AAB34044.1; -; mRNA.
DR PIR; S54713; S54713.
DR AlphaFoldDB; Q26427; -.
DR Proteomes; UP000504614; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR008660; L-fibroin.
DR Pfam; PF05849; L-fibroin; 1.
DR PIRSF; PIRSF005765; L-fibroin; 1.
PE 1: Evidence at protein level;
KW Acetylation; Direct protein sequencing; Disulfide bond; Reference proteome;
KW Secreted; Signal; Silk protein.
FT SIGNAL 1..16
FT /evidence="ECO:0000269|PubMed:7715595"
FT CHAIN 17..267
FT /note="Fibroin light chain"
FT /id="PRO_0000021257"
FT CHAIN 19..267
FT /note="Fibroin light chain, short form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000389560"
FT MOD_RES 19
FT /note="N-acetylserine; in short form"
FT /evidence="ECO:0000250"
FT DISULFID 103..162
FT /evidence="ECO:0000255"
FT DISULFID 195
FT /note="Interchain (with heavy chain)"
FT /evidence="ECO:0000250"
FT CONFLICT 24
FT /note="Q -> E (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 267 AA; 27079 MW; 2A01050F9F120F6F CRC64;
MLPFVLVLLV ATSALAAPSV VISQDNINNI APRVGNGRPI SSALIDRAFE IVDGGDTNIY
ILTIQQILND LADQPDGLSQ SLAVTQAVAA LGELATGVPG NSCEAAAVID AYANSVRTGD
NSALSIAVAN YINRLSSNIG LISQLASNPD SLRYSSGPAG NCAGGGRSYQ FEAAWDAVLN
NANPYQIGLI NEEYCAARRL YNAFNSRSNN VGAAITAGAV VAQTQAAQII LPSLVNVLSA
VAAGGNVAGA AAQAGQALAN AAANVQL