FIE2_ORYSJ
ID FIE2_ORYSJ Reviewed; 376 AA.
AC Q6ZJX0; A1KXU8;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Polycomb group protein FIE1 {ECO:0000305};
DE AltName: Full=Protein FERTILIZATION-INDEPENDENT ENDOSPERM 2 {ECO:0000303|PubMed:19825651};
DE Short=OsFIE2 {ECO:0000303|PubMed:19825651};
DE AltName: Full=WD40 repeat-containing protein 153 {ECO:0000303|PubMed:22429805};
DE Short=OsWD40-153 {ECO:0000303|PubMed:22429805};
GN Name=FIE2 {ECO:0000303|PubMed:19825651};
GN OrderedLocusNames=Os08g0137100 {ECO:0000312|EMBL:BAF22867.1},
GN LOC_Os08g04270 {ECO:0000305};
GN ORFNames=OJ1613_G04.20 {ECO:0000312|EMBL:BAD03073.1},
GN OsJ_25971 {ECO:0000312|EMBL:EEE68010.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH EZ1.
RX DOI=10.1007/s11816-012-0229-0;
RA Na J.K., Seo M.H., Yoon I.S., Lee Y.H., Lee K.O., Kim D.Y.;
RT "Involvement of rice polycomb protein OsFIE2 in plant growth and seed
RT size.";
RL Plant Biotechnol. Rep. 6:339-346(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=19825651; DOI=10.1093/mp/ssp036;
RA Luo M., Platten D., Chaudhury A., Peacock W.J., Dennis E.S.;
RT "Expression, imprinting, and evolution of rice homologs of the polycomb
RT group genes.";
RL Mol. Plant 2:711-723(2009).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=22429805; DOI=10.1186/1471-2164-13-100;
RA Ouyang Y., Huang X., Lu Z., Yao J.;
RT "Genomic survey, expression profile and co-expression network analysis of
RT OsWD40 family in rice.";
RL BMC Genomics 13:100-100(2012).
RN [9]
RP INTERACTION WITH EZ1.
RX PubMed=23150632; DOI=10.1105/tpc.112.102269;
RA Zhang L., Cheng Z., Qin R., Qiu Y., Wang J.L., Cui X., Gu L., Zhang X.,
RA Guo X., Wang D., Jiang L., Wu C.Y., Wang H., Cao X., Wan J.;
RT "Identification and characterization of an epi-allele of FIE1 reveals a
RT regulatory linkage between two epigenetic marks in rice.";
RL Plant Cell 24:4407-4421(2012).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT, AND INTERACTION
RP WITH EZ1.
RX PubMed=23505380; DOI=10.1371/journal.pgen.1003322;
RA Nallamilli B.R., Zhang J., Mujahid H., Malone B.M., Bridges S.M., Peng Z.;
RT "Polycomb group gene OsFIE2 regulates rice (Oryza sativa) seed development
RT and grain filling via a mechanism distinct from Arabidopsis.";
RL PLoS Genet. 9:E1003322-E1003322(2013).
RN [11]
RP FUNCTION.
RX PubMed=24020752; DOI=10.1111/nph.12472;
RA Li S., Zhou B., Peng X., Kuang Q., Huang X., Yao J., Du B., Sun M.X.;
RT "OsFIE2 plays an essential role in the regulation of rice vegetative and
RT reproductive development.";
RL New Phytol. 201:66-79(2014).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They act via the
CC methylation of histones, rendering chromatin heritably changed in its
CC expressibility (PubMed:23505380, PubMed:24020752). Involved in the
CC regulation of seed endosperm development, grain filling and seed
CC dormancy. FIE2-containing PcG complex in seed endosperm regulates the
CC expression of various transcription factors by trimethylation on
CC histone H3 'Lys-27' (H3K27me3) of target genes. Involved in the overall
CC expression regulation of a large number of nutrient metabolism genes
CC (PubMed:23505380). Involved in the regulation of seed endosperm
CC development. Involved in the regulation of vegetative development,
CC particulary in stem cell maintenance in the root system, where it
CC maintains the suppression of key differentiation regulators
CC (PubMed:24020752). {ECO:0000269|PubMed:23505380,
CC ECO:0000269|PubMed:24020752}.
CC -!- SUBUNIT: Interacts with EZ1 (Ref.1, PubMed:23150632, PubMed:23505380).
CC Component of the polycomb repressive complex 2 (PRC2), composed of the
CC core PRC2 components EMF2B, EZ1 and CLF. PRC2 methylates 'Lys-27'
CC residues of histone H3 (H3K27me3), leading to transcriptional
CC repression of the affected target gene (PubMed:23505380).
CC {ECO:0000269|PubMed:23150632, ECO:0000269|PubMed:23505380,
CC ECO:0000269|Ref.1}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:19825651,
CC ECO:0000269|PubMed:22429805}.
CC -!- MISCELLANEOUS: Plants silencing FIE2 have small and partially filled
CC seeds, and exhibit seed germination before seed maturation
CC (PubMed:23505380). Plant silencing FIE2 exhibit aberrant pleiotropic
CC phenotypes in vegetative and reproductive organ generation, and
CC derepression of some developmental regulators, such as MADS3 and OSH5
CC genes (PubMed:24020752). {ECO:0000269|PubMed:23505380,
CC ECO:0000269|PubMed:24020752}.
CC -!- SIMILARITY: Belongs to the WD repeat ESC family. {ECO:0000305}.
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DR EMBL; AY456262; AAS13489.1; -; mRNA.
DR EMBL; AP003896; BAD03073.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF22867.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT03750.1; -; Genomic_DNA.
DR EMBL; CM000145; EEE68010.1; -; Genomic_DNA.
DR EMBL; AK111761; BAG99405.1; -; mRNA.
DR RefSeq; XP_015649884.1; XM_015794398.1.
DR RefSeq; XP_015649885.1; XM_015794399.1.
DR AlphaFoldDB; Q6ZJX0; -.
DR SMR; Q6ZJX0; -.
DR STRING; 4530.OS08T0137100-01; -.
DR PaxDb; Q6ZJX0; -.
DR PRIDE; Q6ZJX0; -.
DR EnsemblPlants; Os08t0137100-01; Os08t0137100-01; Os08g0137100.
DR GeneID; 4344620; -.
DR Gramene; Os08t0137100-01; Os08t0137100-01; Os08g0137100.
DR eggNOG; KOG1034; Eukaryota.
DR HOGENOM; CLU_032683_2_0_1; -.
DR InParanoid; Q6ZJX0; -.
DR OMA; WSYDSVT; -.
DR OrthoDB; 1191277at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0005677; C:chromatin silencing complex; IMP:UniProtKB.
DR GO; GO:0035098; C:ESC/E(Z) complex; IBA:GO_Central.
DR GO; GO:0031519; C:PcG protein complex; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009960; P:endosperm development; IMP:UniProtKB.
DR GO; GO:0031507; P:heterochromatin assembly; IMP:UniProtKB.
DR GO; GO:0070734; P:histone H3-K27 methylation; IMP:UniProtKB.
DR GO; GO:0010231; P:maintenance of seed dormancy; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0090696; P:post-embryonic plant organ development; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Developmental protein; Differentiation;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW WD repeat.
FT CHAIN 1..376
FT /note="Polycomb group protein FIE1"
FT /id="PRO_0000444462"
FT REPEAT 85..127
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 130..170
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 176..216
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 242..279
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 291..332
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 339..376
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT CONFLICT 50
FT /note="F -> V (in Ref. 1; AAS13489)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 42036 MW; 1B0697B9A57A322F CRC64;
MAKLGPGQGL GCEAAVGLLA PSRKREYKAC NKLTEGKRPL YAIGFNFLDF HYYEVFATVG
GNRVTTYSCL KDGNFAILQA YIDEDKDESF YTLSWACDLD GTPLLVAAGS NGIIRVINCA
TEKLLKTFVG HGDSINEIRT QALKPSLIIS ASKDESVRLW NVHTGICILI FAGAGGHRNE
VLSVDFHPSD IYRIASCGMD NTVKIWSMKE FWPYVEQSFT WTDLPSKFPT KYVQFPVLVA
VVHSNYVDCT RWLGDFILSK SVDNEIVLWE PKTKEQSPGE GSIDILQKYP VPECDIWFIK
FSCDFHFNQL AIGNREGKVF VWEVQSSPPV LTARLTNPQC KSAIRQTAVS FDGSTILACS
EDGSIWRWDE VDHPKA
