FIE_ARATH
ID FIE_ARATH Reviewed; 369 AA.
AC Q9LT47; Q9XF44;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2003, sequence version 2.
DT 25-MAY-2022, entry version 163.
DE RecName: Full=Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM;
DE AltName: Full=Protein FERTILIZATION-INDEPENDENT SEED 3;
GN Name=FIE; Synonyms=FIS3; OrderedLocusNames=At3g20740; ORFNames=MOE17.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS
RP OF 365-ASP--LYS-369.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=10072400; DOI=10.2307/3870869;
RA Ohad N., Yadegari R., Margossian L., Hannon M., Michaeli D., Harada J.J.,
RA Goldberg R.B., Fischer R.L.;
RT "Mutations in FIE, a WD polycomb group gene, allow endosperm development
RT without fertilization.";
RL Plant Cell 11:407-416(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP INTERACTION WITH MEA, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11114524; DOI=10.1016/s0960-9822(00)00839-3;
RA Spillane C., MacDougall C., Stock C., Koehler C., Vielle-Calzada J.-P.,
RA Nunes S.M., Grossniklaus U., Goodrich J.;
RT "Interaction of the Arabidopsis polycomb group proteins FIE and MEA
RT mediates their common phenotypes.";
RL Curr. Biol. 10:1535-1538(2000).
RN [7]
RP INTERACTION WITH MEA, AND TISSUE SPECIFICITY.
RX PubMed=10962025; DOI=10.1073/pnas.170292997;
RA Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.;
RT "Expression and parent-of-origin effects for FIS2, MEA, and FIE in the
RT endosperm and embryo of developing Arabidopsis seeds.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000).
RN [8]
RP INTERACTION WITH MEA, AND TISSUE SPECIFICITY.
RX PubMed=11148284; DOI=10.2307/3871235;
RA Yadegari R., Kinoshita T., Lotan O., Cohen G., Katz A., Choi Y., Katz A.,
RA Nakashima K., Harada J.J., Goldberg R.B., Fischer R.L., Ohad N.;
RT "Mutations in the FIE and MEA genes that encode interacting polycomb
RT proteins cause parent-of-origin effects on seed development by distinct
RT mechanisms.";
RL Plant Cell 12:2367-2382(2000).
RN [9]
RP FUNCTION.
RX PubMed=11250158; DOI=10.1016/s0960-9822(01)00072-0;
RA Soerensen M.B., Chaudhury A.M., Robert H., Bancharel E., Berger F.;
RT "Polycomb group genes control pattern formation in plant seed.";
RL Curr. Biol. 11:277-281(2001).
RN [10]
RP FUNCTION.
RX PubMed=12815071; DOI=10.1101/gad.257403;
RA Koehler C., Hennig L., Spillane C., Pien S., Gruissem W., Grossniklaus U.;
RT "The Polycomb-group protein MEDEA regulates seed development by controlling
RT expression of the MADS-box gene PHERES1.";
RL Genes Dev. 17:1540-1553(2003).
RN [11]
RP FUNCTION.
RX PubMed=15151989; DOI=10.1242/dev.01168;
RA Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E.,
RA Grossniklaus U., Berger F.;
RT "Identification of new members of fertilisation independent seed Polycomb
RT group pathway involved in the control of seed development in Arabidopsis
RT thaliana.";
RL Development 131:2971-2981(2004).
RN [12]
RP INTERACTION WITH CLF.
RX PubMed=14871310; DOI=10.1111/j.1365-313x.2003.01996.x;
RA Katz A., Oliva M., Mosquna A., Hakim O., Ohad N.;
RT "FIE and CURLY LEAF polycomb proteins interact in the regulation of
RT homeobox gene expression during sporophyte development.";
RL Plant J. 37:707-719(2004).
RN [13]
RP SUBUNIT, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18854416; DOI=10.1073/pnas.0808687105;
RA De Lucia F., Crevillen P., Jones A.M.E., Greb T., Dean C.;
RT "A PHD-polycomb repressive complex 2 triggers the epigenetic silencing of
RT FLC during vernalization.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:16831-16836(2008).
RN [14]
RP INTERACTION WITH ALP1.
RX PubMed=26642436; DOI=10.1371/journal.pgen.1005660;
RA Liang S.C., Hartwig B., Perera P., Mora-Garcia S., de Leau E., Thornton H.,
RA de Lima Alves F., de Alves F.L., Rappsilber J., Rapsilber J., Yang S.,
RA James G.V., Schneeberger K., Finnegan E.J., Turck F., Goodrich J.;
RT "Kicking against the PRCs - A domesticated transposase antagonises
RT silencing mediated by polycomb group proteins and is an accessory component
RT of polycomb repressive complex 2.";
RL PLoS Genet. 11:E1005660-E1005660(2015).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They probably act
CC via the methylation of histones, rendering chromatin heritably changed
CC in its expressibility. Required to prevent the proliferation of the
CC central cell by repressing unknown target genes before fertilization.
CC Probably also involved in floral repression mechanism established
CC during early plant development. Regulates the anteroposterior
CC organization of the endosperm. Interacts with the promoter and
CC represses the transcription of genes such as PHE1, that are paternally
CC active and maternally silenced. {ECO:0000269|PubMed:10072400,
CC ECO:0000269|PubMed:11250158, ECO:0000269|PubMed:12815071,
CC ECO:0000269|PubMed:15151989}.
CC -!- SUBUNIT: Interacts directly with MEA. These two proteins are probably
CC indirectly associated with FIS2. In plants, PcG complexes are probably
CC composed of a member of the EZ family (CLF or MEA), FIE, and a member
CC of the VEFS family (FIS2, VRN2 or EMF2). Component of the plant
CC homeodomain / polycomb repressive complex 2 (PHD-PRC2) large complex
CC during prolonged cold, composed of core PRC2 components (VRN2, EZA1,
CC FIE and MSI1), and three related PHD finger proteins (VIL1, VIL2 and
CC VIN3) that mediates histone H3 trimethylation on 'Lys-27' (H3K27me3).
CC Binds to ALP1 (PubMed:26642436). {ECO:0000269|PubMed:10962025,
CC ECO:0000269|PubMed:11114524, ECO:0000269|PubMed:11148284,
CC ECO:0000269|PubMed:14871310, ECO:0000269|PubMed:18854416,
CC ECO:0000269|PubMed:26642436}.
CC -!- INTERACTION:
CC Q9LT47; P93831: CLF; NbExp=4; IntAct=EBI-307146, EBI-307155;
CC Q9LT47; O65312: MEA; NbExp=12; IntAct=EBI-307146, EBI-632832;
CC Q9LT47; O22467: MSI1; NbExp=6; IntAct=EBI-307146, EBI-632891;
CC Q9LT47; Q9LKZ3: RBR1; NbExp=2; IntAct=EBI-307146, EBI-398590;
CC Q9LT47; Q8W5B1: VRN2; NbExp=3; IntAct=EBI-307146, EBI-2128880;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11114524}.
CC Note=Excluded from the nucleolus.
CC -!- TISSUE SPECIFICITY: Expressed in cauline leaves, root and stems. In the
CC male reproductive organ, it is expressed in the developing anther; and
CC is abundant in microspore mother cells, in microsporocytes and in the
CC tapetum, but is absent from vascular bundles, the connective tissue and
CC the filament. It is also absent from pollen grains at subsequent
CC developmental stages. In the developing female reproductive organs, it
CC is highly expressed in all cells of the young ovules primordium before
CC archesporial differentiation. Then, it is highly expressed in the ovule
CC sporophytic tissue and the megaspore mother cell before meiosis, but is
CC absent from placenta or the developing carpel. Then, it decreases.
CC {ECO:0000269|PubMed:10072400, ECO:0000269|PubMed:10962025,
CC ECO:0000269|PubMed:11114524, ECO:0000269|PubMed:11148284}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically. Expressed in
CC both egg and central cell before fertilization, and in the embryo and
CC endosperm after fertilization.
CC -!- SIMILARITY: Belongs to the WD repeat ESC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02481.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF129516; AAD23584.1; -; mRNA.
DR EMBL; AB025629; BAB02481.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76418.1; -; Genomic_DNA.
DR EMBL; AK117114; BAC41793.1; -; mRNA.
DR EMBL; BT005326; AAO63390.1; -; mRNA.
DR RefSeq; NP_188710.1; NM_112965.1.
DR AlphaFoldDB; Q9LT47; -.
DR SMR; Q9LT47; -.
DR BioGRID; 6954; 11.
DR DIP; DIP-31378N; -.
DR IntAct; Q9LT47; 11.
DR STRING; 3702.AT3G20740.1; -.
DR iPTMnet; Q9LT47; -.
DR PaxDb; Q9LT47; -.
DR PRIDE; Q9LT47; -.
DR ProteomicsDB; 230511; -.
DR EnsemblPlants; AT3G20740.1; AT3G20740.1; AT3G20740.
DR GeneID; 821622; -.
DR Gramene; AT3G20740.1; AT3G20740.1; AT3G20740.
DR KEGG; ath:AT3G20740; -.
DR Araport; AT3G20740; -.
DR TAIR; locus:2091876; AT3G20740.
DR eggNOG; KOG1034; Eukaryota.
DR HOGENOM; CLU_032683_2_0_1; -.
DR InParanoid; Q9LT47; -.
DR OMA; WSYDSVT; -.
DR OrthoDB; 1191277at2759; -.
DR PhylomeDB; Q9LT47; -.
DR PRO; PR:Q9LT47; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LT47; baseline and differential.
DR Genevisible; Q9LT47; AT.
DR GO; GO:0005677; C:chromatin silencing complex; IDA:UniProtKB.
DR GO; GO:0035098; C:ESC/E(Z) complex; IBA:GO_Central.
DR GO; GO:0043078; C:polar nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0016571; P:histone methylation; IMP:TAIR.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:2000014; P:regulation of endosperm development; IMP:TAIR.
DR GO; GO:0006349; P:regulation of gene expression by genomic imprinting; IMP:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0010048; P:vernalization response; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Developmental protein; Nucleus; Reference proteome;
KW Repeat; Repressor; Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..369
FT /note="Polycomb group protein FERTILIZATION-INDEPENDENT
FT ENDOSPERM"
FT /id="PRO_0000050977"
FT REPEAT 31..73
FT /note="WD 1"
FT REPEAT 81..123
FT /note="WD 2"
FT REPEAT 126..166
FT /note="WD 3"
FT REPEAT 172..212
FT /note="WD 4"
FT REPEAT 238..275
FT /note="WD 5"
FT REPEAT 287..328
FT /note="WD 6"
FT REPEAT 335..368
FT /note="WD 7"
FT MUTAGEN 365..369
FT /note="Missing: In fie-6; induces endosperm proliferation
FT without fertilization. Abolishes interaction with MEA."
FT /evidence="ECO:0000269|PubMed:10072400"
SQ SEQUENCE 369 AA; 41257 MW; EDF0989C02B4FD4A CRC64;
MSKITLGNES IVGSLTPSNK KSYKVTNRIQ EGKKPLYAVV FNFLDARFFD VFVTAGGNRI
TLYNCLGDGA ISALQSYADE DKEESFYTVS WACGVNGNPY VAAGGVKGII RVIDVNSETI
HKSLVGHGDS VNEIRTQPLK PQLVITASKD ESVRLWNVET GICILIFAGA GGHRYEVLSV
DFHPSDIYRF ASCGMDTTIK IWSMKEFWTY VEKSFTWTDD PSKFPTKFVQ FPVFTASIHT
NYVDCNRWFG DFILSKSVDN EILLWEPQLK ENSPGEGASD VLLRYPVPMC DIWFIKFSCD
LHLSSVAIGN QEGKVYVWDL KSCPPVLITK LSHNQSKSVI RQTAMSVDGS TILACCEDGT
IWRWDVITK
