ID   FIG01_BOARA             Reviewed;          68 AA.
AC   A0A2L2DDE6;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-APR-2018, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Figainin 1 {ECO:0000303|PubMed:32967114};
DE   AltName: Full=Br24 {ECO:0000303|PubMed:32967114};
DE   Flags: Precursor;
OS   Boana raniceps (Chaco tree frog) (Hyla roeschmanni).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Hylinae; Cophomantini;
OC   Boana.
OX   NCBI_TaxID=192750 {ECO:0000312|EMBL:AVG44202.1};
RN   [1] {ECO:0000312|EMBL:AVG44202.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Barbosa E.A., Campos P.F., Andrade A.C., Bloch C.;
RT   "Response of Hypsiboas raniceps to abiotic and biotic stresses: gene
RT   expression and MALDI-mass spectrometry imaging analysis of skin peptides.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 50-67, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION AT LYS-67.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:32967114};
RX   PubMed=32967114; DOI=10.3390/antibiotics9090625;
RA   Santana C.J.C., Magalhaes A.C.M., Dos Santos Junior A.C.M., Ricart C.A.O.,
RA   Lima B.D., Alvares A.D.C.M., Freitas S.M., Pires O.R. Jr., Fontes W.,
RA   Castro M.S.;
RT   "Figainin 1, a Novel Amphibian Skin Peptide with Antimicrobial and
RT   Antiproliferative Properties.";
RL   Antibiotics 9:0-0(2020).
CC   -!- FUNCTION: Antimicrobial peptide that displays antibacterial and
CC       antiprotozoal activity (PubMed:32967114). Exhibits antibacterial
CC       activity against the Gram-positive bacteria S.epidermidis ATCC 12228
CC       (MIC=2 uM), E.casseliflavus ATCC 700327 (MIC=16 uM), S.aureus ATCC
CC       25923 (MIC=4 uM) and E.faecalis ATCC 29212 (MIC=8 uM), and the Gram-
CC       negative bacteria E.coli ATCC 25922 (MIC=16 uM) and K.pneumoniae ATCC
CC       13883 (MIC=4 uM) (PubMed:32967114). Displays antiprotozoal activity
CC       against the epimastigote form of T.cruzi (IC(50)=15.9 uM)
CC       (PubMed:32967114). Does not show antimicrobial activity against the
CC       Gram-negative bacterium P.aeruginosa ATCC 27853, or the fungi
CC       C.albicans ATCC 90028 and C.parapsilosis ATCC 22019 (PubMed:32967114).
CC       Shows high cytolytic activity against human erythrocytes (HC(50)=10
CC       uM), and displays anti-proliferative effects against various cancer
CC       cell lines including MCF-7 breast cancer cells (IC(50)=13.7 uM), HeLa
CC       cervical adenocarcinoma cells (IC(50)=11.1 uM) and B16F10 murine
CC       melanoma cells (IC(50)=10.5 uM) (PubMed:32967114).
CC       {ECO:0000269|PubMed:32967114}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32967114}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:32967114}.
CC   -!- MASS SPECTROMETRY: Mass=1915.2; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:32967114};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       {ECO:0000305}.
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DR   EMBL; KY748198; AVG44202.1; -; mRNA.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0042832; P:defense response to protozoan; IDA:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Cytolysis; Direct protein sequencing;
KW   Hemolysis; Immunity; Innate immunity; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..47
FT                   /evidence="ECO:0000269|PubMed:32967114"
FT                   /id="PRO_0000455243"
FT   PEPTIDE         50..67
FT                   /note="Figainin 1"
FT                   /evidence="ECO:0000269|PubMed:32967114"
FT                   /id="PRO_0000455244"
FT   REGION          23..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         67
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:32967114"
SQ   SEQUENCE   68 AA;  7640 MW;  7900396B456D9E2D CRC64;
     MAFLKKSLFL VLFLGLVSLS IGEEEKREEE EKNEEGANQE ENAENKEKRF IGTLIPLALG
     ALTKLFKG