FII_LAMBD
ID FII_LAMBD Reviewed; 117 AA.
AC P03714;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 29-SEP-2021, entry version 112.
DE RecName: Full=Head-tail connector protein FII {ECO:0000303|PubMed:20660769};
DE AltName: Full=Head-tail joining protein {ECO:0000303|PubMed:12083526};
DE AltName: Full=Minor capsid protein FII;
GN Name=FII; OrderedLocusNames=lambdap10;
OS Escherichia phage lambda (Bacteriophage lambda).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus.
OX NCBI_TaxID=10710;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT "Nucleotide sequence of bacteriophage lambda DNA.";
RL J. Mol. Biol. 162:729-773(1982).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=5022189; DOI=10.1016/0022-2836(72)90082-4;
RA Casjens S., Horn T., Kaiser A.D.;
RT "Head assembly steps controlled by genes F and W in bacteriophage lambda.";
RL J. Mol. Biol. 64:551-563(1972).
RN [3]
RP FUNCTION.
RX PubMed=4413519; DOI=10.1016/0042-6822(74)90238-4;
RA Boklage C.E., Wong E.C., Bode V.C.;
RT "Functional abnormality of lambda phage particles from complemented FII-
RT mutant lysates.";
RL Virology 61:22-28(1974).
RN [4]
RP FUNCTION.
RX PubMed=4616093; DOI=10.1016/0022-2836(74)90252-6;
RA Casjens S.;
RT "Bacteriophage lambda FII gene protein: role in head assembly.";
RL J. Mol. Biol. 90:1-20(1974).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=12083526; DOI=10.1016/s0022-2836(02)00276-0;
RA Maxwell K.L., Yee A.A., Arrowsmith C.H., Gold M., Davidson A.R.;
RT "The solution structure of the bacteriophage lambda head-tail joining
RT protein, gpFII.";
RL J. Mol. Biol. 318:1395-1404(2002).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=20660769; DOI=10.1073/pnas.1005822107;
RA Cardarelli L., Pell L.G., Neudecker P., Pirani N., Liu A., Baker L.A.,
RA Rubinstein J.L., Maxwell K.L., Davidson A.R.;
RT "Phages have adapted the same protein fold to fulfill multiple functions in
RT virion assembly.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:14384-14389(2010).
CC -!- FUNCTION: Plays a role in virion assembly by joining the head and the
CC tail at the last step of morphogenesis. Six FII proteins probably bind
CC protein W that closes the DNA-filled capsid, as well as the U tail
CC terminator protein of the pre-assembled tail.
CC {ECO:0000269|PubMed:4413519, ECO:0000269|PubMed:4616093,
CC ECO:0000269|PubMed:5022189}.
CC -!- SUBUNIT: May bind head-to-tail joining protein W and protein U.
CC {ECO:0000269|PubMed:5022189}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:5022189}. Host
CC cytoplasm {ECO:0000269|PubMed:5022189}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-23 is the initiator.
CC {ECO:0000305}.
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DR EMBL; J02459; AAA96542.1; -; Genomic_DNA.
DR PIR; I94614; VHBPFL.
DR RefSeq; NP_040589.1; NC_001416.1.
DR PDB; 1K0H; NMR; -; A=1-117.
DR PDB; 2KX4; NMR; -; A=1-117.
DR PDBsum; 1K0H; -.
DR PDBsum; 2KX4; -.
DR SMR; P03714; -.
DR GeneID; 2703484; -.
DR KEGG; vg:2703484; -.
DR EvolutionaryTrace; P03714; -.
DR Proteomes; UP000001711; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019068; P:virion assembly; IEA:InterPro.
DR DisProt; DP01762; -.
DR InterPro; IPR008018; Phage_tail_attach_FII.
DR Pfam; PF05354; Phage_attach; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host cytoplasm; Late protein; Reference proteome;
KW Viral capsid assembly; Viral release from host cell; Virion.
FT CHAIN 1..117
FT /note="Head-tail connector protein FII"
FT /id="PRO_0000077599"
FT STRAND 5..7
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 9..15
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:1K0H"
FT TURN 32..34
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 37..44
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 64..71
FT /evidence="ECO:0007829|PDB:1K0H"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 80..83
FT /evidence="ECO:0007829|PDB:1K0H"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 95..97
FT /evidence="ECO:0007829|PDB:1K0H"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:2KX4"
SQ SEQUENCE 117 AA; 12761 MW; E70088E7B17A5A2B CRC64;
MADFDNLFDA AIARADETIR GYMGTSATIT SGEQSGAVIR GVFDDPENIS YAGQGVRVEG
SSPSLFVRTD EVRQLRRGDT LTIGEENFWV DRVSPDDGGS CHLWLGRGVP PAVNRRR
