FIMA1_ECOLI
ID FIMA1_ECOLI Reviewed; 182 AA.
AC P04128; Q2M5Z9;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Type-1 fimbrial protein, A chain;
DE AltName: Full=Type-1A pilin;
DE Flags: Precursor;
GN Name=fimA; Synonyms=pilA; OrderedLocusNames=b4314, JW4277;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2858471; DOI=10.1128/jb.162.1.454-457.1985;
RA Orndorff P.E., Falkow S.;
RT "Nucleotide sequence of pilA, the gene encoding the structural component of
RT type 1 pili in Escherichia coli.";
RL J. Bacteriol. 162:454-457(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6147250; DOI=10.1111/j.1432-1033.1984.tb08386.x;
RA Klemm P.;
RT "The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli.
RT Nucleotide sequence and primary structure of the protein.";
RL Eur. J. Biochem. 143:395-399(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Fimbriae (also called pili), polar filaments radiating from
CC the surface of the bacterium to a length of 0.5-1.5 micrometers and
CC numbering 100-300 per cell, enable bacteria to colonize the epithelium
CC of specific host organs.
CC -!- INTERACTION:
CC P04128; P04128: fimA; NbExp=2; IntAct=EBI-15697528, EBI-15697528;
CC -!- SUBCELLULAR LOCATION: Fimbrium.
CC -!- SIMILARITY: Belongs to the fimbrial protein family. {ECO:0000305}.
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DR EMBL; X00981; CAA25489.1; -; Genomic_DNA.
DR EMBL; M27603; AAA24389.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97210.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77270.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78307.1; -; Genomic_DNA.
DR PIR; S56539; YQECT1.
DR RefSeq; NP_418734.1; NC_000913.3.
DR RefSeq; WP_000695564.1; NZ_LN832404.1.
DR PDB; 2JTY; NMR; -; A=24-182.
DR PDB; 2M5G; NMR; -; A=24-182.
DR PDB; 2N7H; NMR; -; A/B/C/D/E/F=24-182.
DR PDB; 3SQB; X-ray; 3.20 A; B/D/F/H=37-182.
DR PDB; 4DWH; X-ray; 2.50 A; B/D=41-182.
DR PDB; 5NKT; X-ray; 1.50 A; A=24-182.
DR PDB; 5OH0; EM; 4.20 A; A/B/C/D/E/F=24-182.
DR PDB; 6C53; EM; 4.20 A; A/B/C/D/E/F/G/H/I/J/K=24-182.
DR PDB; 6R74; X-ray; 1.81 A; A=42-182.
DR PDB; 6R7E; X-ray; 1.79 A; A=31-182.
DR PDB; 6S09; X-ray; 1.50 A; A/B/C/D/E/F/G/H=42-182.
DR PDB; 6SWH; X-ray; 2.80 A; C/F=37-182.
DR PDB; 6Y7S; EM; 2.80 A; A/B/C/D/E/F=1-182.
DR PDBsum; 2JTY; -.
DR PDBsum; 2M5G; -.
DR PDBsum; 2N7H; -.
DR PDBsum; 3SQB; -.
DR PDBsum; 4DWH; -.
DR PDBsum; 5NKT; -.
DR PDBsum; 5OH0; -.
DR PDBsum; 6C53; -.
DR PDBsum; 6R74; -.
DR PDBsum; 6R7E; -.
DR PDBsum; 6S09; -.
DR PDBsum; 6SWH; -.
DR PDBsum; 6Y7S; -.
DR AlphaFoldDB; P04128; -.
DR BMRB; P04128; -.
DR SMR; P04128; -.
DR BioGRID; 4262746; 9.
DR DIP; DIP-9609N; -.
DR IntAct; P04128; 2.
DR STRING; 511145.b4314; -.
DR jPOST; P04128; -.
DR PaxDb; P04128; -.
DR PRIDE; P04128; -.
DR EnsemblBacteria; AAC77270; AAC77270; b4314.
DR EnsemblBacteria; BAE78307; BAE78307; BAE78307.
DR GeneID; 948838; -.
DR KEGG; ecj:JW4277; -.
DR KEGG; eco:b4314; -.
DR PATRIC; fig|1411691.4.peg.2378; -.
DR EchoBASE; EB0304; -.
DR eggNOG; COG3539; Bacteria.
DR HOGENOM; CLU_088965_0_0_6; -.
DR InParanoid; P04128; -.
DR OMA; QLNDCDS; -.
DR PhylomeDB; P04128; -.
DR BioCyc; EcoCyc:EG10308-MON; -.
DR EvolutionaryTrace; P04128; -.
DR PRO; PR:P04128; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009289; C:pilus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0007155; P:cell adhesion; IDA:EcoCyc.
DR GO; GO:0043709; P:cell adhesion involved in single-species biofilm formation; IBA:GO_Central.
DR Gene3D; 2.60.40.1090; -; 1.
DR InterPro; IPR000259; Adhesion_dom_fimbrial.
DR InterPro; IPR036937; Adhesion_dom_fimbrial_sf.
DR InterPro; IPR008966; Adhesion_dom_sf.
DR Pfam; PF00419; Fimbrial; 1.
DR SUPFAM; SSF49401; SSF49401; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Fimbrium; Reference proteome; Signal.
FT SIGNAL 1..23
FT CHAIN 24..182
FT /note="Type-1 fimbrial protein, A chain"
FT /id="PRO_0000009170"
FT DISULFID 44..84
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="A -> T (in Ref. 1; AAA24389)"
FT /evidence="ECO:0000305"
FT CONFLICT 163
FT /note="Missing (in Ref. 2; CAA25489)"
FT /evidence="ECO:0000305"
FT STRAND 31..37
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:5NKT"
FT TURN 48..51
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 52..58
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:6S09"
FT HELIX 62..65
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 67..71
FT /evidence="ECO:0007829|PDB:4DWH"
FT STRAND 75..84
FT /evidence="ECO:0007829|PDB:5NKT"
FT TURN 86..88
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 90..97
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:6S09"
FT TURN 115..117
FT /evidence="ECO:0007829|PDB:6Y7S"
FT STRAND 120..127
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 131..134
FT /evidence="ECO:0007829|PDB:2N7H"
FT STRAND 137..141
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 149..167
FT /evidence="ECO:0007829|PDB:5NKT"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:6SWH"
FT STRAND 172..181
FT /evidence="ECO:0007829|PDB:5NKT"
SQ SEQUENCE 182 AA; 18111 MW; 35B2016BDD21A2C1 CRC64;
MKIKTLAIVV LSALSLSSTA ALAAATTVNG GTVHFKGEVV NAACAVDAGS VDQTVQLGQV
RTASLAQEGA TSSAVGFNIQ LNDCDTNVAS KAAVAFLGTA IDAGHTNVLA LQSSAAGSAT
NVGVQILDRT GAALTLDGAT FSSETTLNNG TNTIPFQARY FATGAATPGA ANADATFKVQ
YQ
