FIMD_ECOLI
ID FIMD_ECOLI Reviewed; 878 AA.
AC P30130; Q2M5Z6;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Outer membrane usher protein FimD;
DE Flags: Precursor;
GN Name=fimD; OrderedLocusNames=b4317, JW5780;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1970114; DOI=10.1007/bf00260505;
RA Klemm P., Christiansen G.;
RT "The fimD gene required for cell surface localization of Escherichia coli
RT type 1 fimbriae.";
RL Mol. Gen. Genet. 220:334-338(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF N-TERMINUS.
RX PubMed=12842468; DOI=10.1016/s0022-2836(03)00591-6;
RA Nishiyama M., Vetsch M., Puorger C., Jelesarov I., Glockshuber R.;
RT "Identification and characterization of the chaperone-subunit complex-
RT binding domain from the type 1 pilus assembly platform FimD.";
RL J. Mol. Biol. 330:513-525(2003).
CC -!- FUNCTION: Involved in the export and assembly of FimA fimbrial subunits
CC across the outer membrane.
CC -!- INTERACTION:
CC P30130; P31697: fimC; NbExp=11; IntAct=EBI-554889, EBI-1028005;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
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DR EMBL; X51655; CAA35968.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97213.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77273.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78310.1; -; Genomic_DNA.
DR PIR; S56542; S56542.
DR RefSeq; NP_418737.1; NC_000913.3.
DR RefSeq; WP_000121005.1; NZ_LN832404.1.
DR PDB; 1ZDV; NMR; -; A=70-184.
DR PDB; 1ZDX; NMR; -; A=70-170.
DR PDB; 1ZE3; X-ray; 1.84 A; D=46-170.
DR PDB; 3BWU; X-ray; 1.76 A; D=46-170.
DR PDB; 3OHN; X-ray; 3.01 A; A/B=169-708.
DR PDB; 3RFZ; X-ray; 2.80 A; B/E=46-878.
DR PDB; 4J3O; X-ray; 3.80 A; D=46-878.
DR PDB; 6E14; EM; 4.00 A; D=1-878.
DR PDB; 6E15; EM; 6.20 A; D=1-878.
DR PDB; 7B0X; X-ray; 1.70 A; D=46-170.
DR PDBsum; 1ZDV; -.
DR PDBsum; 1ZDX; -.
DR PDBsum; 1ZE3; -.
DR PDBsum; 3BWU; -.
DR PDBsum; 3OHN; -.
DR PDBsum; 3RFZ; -.
DR PDBsum; 4J3O; -.
DR PDBsum; 6E14; -.
DR PDBsum; 6E15; -.
DR PDBsum; 7B0X; -.
DR AlphaFoldDB; P30130; -.
DR BMRB; P30130; -.
DR SMR; P30130; -.
DR BioGRID; 4262748; 186.
DR DIP; DIP-9612N; -.
DR IntAct; P30130; 7.
DR MINT; P30130; -.
DR STRING; 511145.b4317; -.
DR TCDB; 1.B.11.3.9; the outer membrane fimbrial usher porin (fup) family.
DR PaxDb; P30130; -.
DR PRIDE; P30130; -.
DR EnsemblBacteria; AAC77273; AAC77273; b4317.
DR EnsemblBacteria; BAE78310; BAE78310; BAE78310.
DR GeneID; 948844; -.
DR KEGG; ecj:JW5780; -.
DR KEGG; eco:b4317; -.
DR PATRIC; fig|1411691.4.peg.2375; -.
DR EchoBASE; EB0307; -.
DR eggNOG; COG3188; Bacteria.
DR HOGENOM; CLU_009120_3_1_6; -.
DR InParanoid; P30130; -.
DR OMA; VPQAFMG; -.
DR PhylomeDB; P30130; -.
DR BioCyc; EcoCyc:EG10311-MON; -.
DR EvolutionaryTrace; P30130; -.
DR PRO; PR:P30130; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015473; F:fimbrial usher porin activity; IDA:EcoCyc.
DR GO; GO:0009297; P:pilus assembly; IDA:EcoCyc.
DR Gene3D; 2.60.40.2070; -; 1.
DR Gene3D; 2.60.40.2610; -; 1.
DR Gene3D; 3.10.20.410; -; 1.
DR InterPro; IPR000015; Fimb_usher.
DR InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR InterPro; IPR042186; FimD_plug_dom.
DR InterPro; IPR025949; PapC-like_C.
DR InterPro; IPR043142; PapC-like_C_sf.
DR InterPro; IPR025885; PapC_N.
DR InterPro; IPR037224; PapC_N_sf.
DR PANTHER; PTHR30451; PTHR30451; 1.
DR Pfam; PF13953; PapC_C; 1.
DR Pfam; PF13954; PapC_N; 1.
DR Pfam; PF00577; Usher; 1.
DR SUPFAM; SSF141729; SSF141729; 1.
DR PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Direct protein sequencing;
KW Disulfide bond; Fimbrium biogenesis; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane beta strand; Transport.
FT SIGNAL 1..45
FT /evidence="ECO:0000269|PubMed:12842468"
FT CHAIN 46..878
FT /note="Outer membrane usher protein FimD"
FT /id="PRO_0000009312"
FT DISULFID 855..877
FT /evidence="ECO:0000255"
FT CONFLICT 143..148
FT /note="QDATAH -> RTLLRT (in Ref. 1; CAA35968)"
FT /evidence="ECO:0000305"
FT CONFLICT 352..402
FT /note="DGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLH
FT -> VLAARRFLPYPIRTFPLLNVKAYSLFHYGRRIPYESRSRKKPAFSRVHYSN (in
FT Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 658..670
FT /note="GGDGNSGSTGYAT -> EAMEIAEVQATP (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 723..742
FT /note="APGAKDAKVENQTGVRTDWR -> RLAQKMQKSKTRRGAYRLA (in Ref.
FT 1; CAA35968)"
FT /evidence="ECO:0000305"
FT HELIX 51..53
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 64..68
FT /evidence="ECO:0007829|PDB:7B0X"
FT STRAND 75..84
FT /evidence="ECO:0007829|PDB:7B0X"
FT STRAND 87..98
FT /evidence="ECO:0007829|PDB:7B0X"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 111..115
FT /evidence="ECO:0007829|PDB:7B0X"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 138..141
FT /evidence="ECO:0007829|PDB:7B0X"
FT TURN 142..144
FT /evidence="ECO:0007829|PDB:1ZE3"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:7B0X"
FT TURN 151..154
FT /evidence="ECO:0007829|PDB:7B0X"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 162..164
FT /evidence="ECO:0007829|PDB:7B0X"
FT HELIX 175..177
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 184..198
FT /evidence="ECO:0007829|PDB:3RFZ"
FT TURN 199..201
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 202..217
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 220..231
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 243..254
FT /evidence="ECO:0007829|PDB:3RFZ"
FT TURN 255..258
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 259..267
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 270..273
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 277..285
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 287..289
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 292..294
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 301..315
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 318..325
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 327..332
FT /evidence="ECO:0007829|PDB:3RFZ"
FT TURN 338..341
FT /evidence="ECO:0007829|PDB:3OHN"
FT STRAND 344..349
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 351..353
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 355..360
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 374..383
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 394..403
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 405..407
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 408..417
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 420..432
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 435..447
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 453..465
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 471..482
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 489..493
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 494..496
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 525..536
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 538..554
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 560..570
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 573..583
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 585..587
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 591..602
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 603..605
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 613..616
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 617..625
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 627..629
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 631..642
FT /evidence="ECO:0007829|PDB:3RFZ"
FT TURN 643..646
FT /evidence="ECO:0007829|PDB:3OHN"
FT STRAND 647..658
FT /evidence="ECO:0007829|PDB:3RFZ"
FT TURN 659..661
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 664..674
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 679..686
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 691..704
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 707..712
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 716..722
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 743..748
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 756..760
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 762..764
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 769..772
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 774..778
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 785..789
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 792..804
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 814..821
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 823..825
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 828..830
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 831..837
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 839..847
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 856..860
FT /evidence="ECO:0007829|PDB:3RFZ"
FT HELIX 863..865
FT /evidence="ECO:0007829|PDB:3RFZ"
FT STRAND 871..875
FT /evidence="ECO:0007829|PDB:3RFZ"
SQ SEQUENCE 878 AA; 96482 MW; B2B86CC44FAB7B0F CRC64;
MSYLNLRLYQ RNTQCLHIRK HRLAGFFVRL VVACAFAAQA PLSSADLYFN PRFLADDPQA
VADLSRFENG QELPPGTYRV DIYLNNGYMA TRDVTFNTGD SEQGIVPCLT RAQLASMGLN
TASVAGMNLL ADDACVPLTT MVQDATAHLD VGQQRLNLTI PQAFMSNRAR GYIPPELWDP
GINAGLLNYN FSGNSVQNRI GGNSHYAYLN LQSGLNIGAW RLRDNTTWSY NSSDRSSGSK
NKWQHINTWL ERDIIPLRSR LTLGDGYTQG DIFDGINFRG AQLASDDNML PDSQRGFAPV
IHGIARGTAQ VTIKQNGYDI YNSTVPPGPF TINDIYAAGN SGDLQVTIKE ADGSTQIFTV
PYSSVPLLQR EGHTRYSITA GEYRSGNAQQ EKTRFFQSTL LHGLPAGWTI YGGTQLADRY
RAFNFGIGKN MGALGALSVD MTQANSTLPD DSQHDGQSVR FLYNKSLNES GTNIQLVGYR
YSTSGYFNFA DTTYSRMNGY NIETQDGVIQ VKPKFTDYYN LAYNKRGKLQ LTVTQQLGRT
STLYLSGSHQ TYWGTSNVDE QFQAGLNTAF EDINWTLSYS LTKNAWQKGR DQMLALNVNI
PFSHWLRSDS KSQWRHASAS YSMSHDLNGR MTNLAGVYGT LLEDNNLSYS VQTGYAGGGD
GNSGSTGYAT LNYRGGYGNA NIGYSHSDDI KQLYYGVSGG VLAHANGVTL GQPLNDTVVL
VKAPGAKDAK VENQTGVRTD WRGYAVLPYA TEYRENRVAL DTNTLADNVD LDNAVANVVP
TRGAIVRAEF KARVGIKLLM TLTHNNKPLP FGAMVTSESS QSSGIVADNG QVYLSGMPLA
GKVQVKWGEE ENAHCVANYQ LPPESQQQLL TQLSAECR
