ID   FIME_ECOL6              Reviewed;         198 AA.
AC   P0ADH8; P04741;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Type 1 fimbriae regulatory protein FimE;
GN   Name=fimE; OrderedLocusNames=c5392;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: FimE is one of the 2 regulatory proteins which control the
CC       phase variation of type 1 fimbriae in E.coli. These proteins mediate
CC       the periodic inversion of a 300bp DNA segment that harbors the promoter
CC       for the fimbrial structural gene, fimA. FimE switches fimA off (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN83814.1; -; Genomic_DNA.
DR   RefSeq; WP_000044711.1; NC_004431.1.
DR   AlphaFoldDB; P0ADH8; -.
DR   SMR; P0ADH8; -.
DR   STRING; 199310.c5392; -.
DR   EnsemblBacteria; AAN83814; AAN83814; c5392.
DR   GeneID; 66671804; -.
DR   KEGG; ecc:c5392; -.
DR   eggNOG; COG4974; Bacteria.
DR   HOGENOM; CLU_027562_39_0_6; -.
DR   OMA; KYLGHQT; -.
DR   BioCyc; ECOL199310:C5392-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Fimbrium biogenesis; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..198
FT                   /note="Type 1 fimbriae regulatory protein FimE"
FT                   /id="PRO_0000197542"
FT   DOMAIN          2..184
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        41
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        136
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        171
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   198 AA;  23116 MW;  A33139B0378CB37D CRC64;
     MSKRRYLTGK EVQAMMQAVC YGATGARDYC LILLAYRHGM RISELLDLHY QDLDLNEGRI
     NIRRLKNGFS TVHPLRFDER EAVERWTQER ANWKGADRTD AIFISRRGSR LSRQQAYRII
     RDAGIEAGTV TQTHPHMLRH ACGYELAERG ADTRLIQDYL GHRNIRHTVR YTASNAARFA
     GLWERNNLIN EKLKREEV