FIME_SHIFL
ID FIME_SHIFL Reviewed; 198 AA.
AC P0ADH9; P04741;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Type 1 fimbriae regulatory protein FimE;
GN Name=fimE; OrderedLocusNames=SF4209, S4466;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: FimE is one of the 2 regulatory proteins which control the
CC phase variation of type 1 fimbriae in E.coli. These proteins mediate
CC the periodic inversion of a 300bp DNA segment that harbors the promoter
CC for the fimbrial structural gene, fimA. FimE switches fimA off (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; AE005674; AAN45629.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP19415.1; -; Genomic_DNA.
DR RefSeq; NP_709922.1; NC_004337.2.
DR RefSeq; WP_000044711.1; NZ_UIQL01000124.1.
DR AlphaFoldDB; P0ADH9; -.
DR SMR; P0ADH9; -.
DR STRING; 198214.SF4209; -.
DR EnsemblBacteria; AAN45629; AAN45629; SF4209.
DR EnsemblBacteria; AAP19415; AAP19415; S4466.
DR GeneID; 1026397; -.
DR GeneID; 66671804; -.
DR KEGG; sfl:SF4209; -.
DR KEGG; sfx:S4466; -.
DR PATRIC; fig|198214.7.peg.4965; -.
DR HOGENOM; CLU_027562_39_0_6; -.
DR OMA; KYLGHQT; -.
DR OrthoDB; 745068at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR Gene3D; 1.10.443.10; -; 1.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; Fimbrium biogenesis;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..198
FT /note="Type 1 fimbriae regulatory protein FimE"
FT /id="PRO_0000197543"
FT DOMAIN 2..184
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 41
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 136
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 139
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 171
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 198 AA; 23116 MW; A33139B0378CB37D CRC64;
MSKRRYLTGK EVQAMMQAVC YGATGARDYC LILLAYRHGM RISELLDLHY QDLDLNEGRI
NIRRLKNGFS TVHPLRFDER EAVERWTQER ANWKGADRTD AIFISRRGSR LSRQQAYRII
RDAGIEAGTV TQTHPHMLRH ACGYELAERG ADTRLIQDYL GHRNIRHTVR YTASNAARFA
GLWERNNLIN EKLKREEV
