FIMP_MOUSE
ID FIMP_MOUSE Reviewed; 111 AA.
AC E9Q9R3; Q8CEL4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Fertilization-influencing membrane protein {ECO:0000303|PubMed:32295885};
DE Flags: Precursor;
GN Name=Fimp {ECO:0000303|PubMed:32295885};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, FUNCTION
RP (ISOFOFRM 1), DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=32295885; DOI=10.1073/pnas.1917060117;
RA Fujihara Y., Lu Y., Noda T., Oji A., Larasati T., Kojima-Kita K., Yu Z.,
RA Matzuk R.M., Matzuk M.M., Ikawa M.;
RT "Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail
RT to fuse with oocytes in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:9393-9400(2020).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: [Isoform 1]: Plays a role in sperm-oocyte fusion process
CC during fertilization. {ECO:0000269|PubMed:32295885}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane
CC {ECO:0000305|PubMed:32295885}; Single-pass type I membrane protein
CC {ECO:0000255}. Note=Localized to the equatorial segment of the sperm
CC head. {ECO:0000269|PubMed:32295885}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Secreted
CC {ECO:0000305|PubMed:32295885}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=E9Q9R3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=E9Q9R3-2; Sequence=VSP_060728, VSP_060729;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:32295885}.
CC -!- DISRUPTION PHENOTYPE: Deficient mice have no overt developmental
CC abnormalities. Furthermore, no deleterious effects on testicular
CC histology and sperm morphology are observed. However male mice are
CC severely subfertile, their spermatozoa show impaired ability to fuse
CC with the oocytes. {ECO:0000269|PubMed:32295885}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH48622.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC25600.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK019625; BAC25600.1; ALT_INIT; mRNA.
DR EMBL; AC124505; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC048622; AAH48622.1; ALT_INIT; mRNA.
DR CCDS; CCDS52402.1; -. [E9Q9R3-1]
DR RefSeq; NP_898954.2; NM_183131.2. [E9Q9R3-1]
DR AlphaFoldDB; E9Q9R3; -.
DR STRING; 10090.ENSMUSP00000049614; -.
DR PhosphoSitePlus; E9Q9R3; -.
DR PaxDb; E9Q9R3; -.
DR ProteomicsDB; 307977; -. [E9Q9R3-1]
DR Ensembl; ENSMUST00000061695; ENSMUSP00000049614; ENSMUSG00000045989. [E9Q9R3-1]
DR GeneID; 78118; -.
DR KEGG; mmu:78118; -.
DR UCSC; uc009jta.1; mouse. [E9Q9R3-1]
DR MGI; MGI:1925368; 4930451I11Rik.
DR VEuPathDB; HostDB:ENSMUSG00000045989; -.
DR eggNOG; ENOG502RVJG; Eukaryota.
DR GeneTree; ENSGT00390000002780; -.
DR HOGENOM; CLU_170753_0_0_1; -.
DR InParanoid; E9Q9R3; -.
DR OrthoDB; 1465806at2759; -.
DR TreeFam; TF338342; -.
DR BioGRID-ORCS; 78118; 2 hits in 71 CRISPR screens.
DR ChiTaRS; 4930451I11Rik; mouse.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; E9Q9R3; protein.
DR Bgee; ENSMUSG00000045989; Expressed in morula and 16 other tissues.
DR ExpressionAtlas; E9Q9R3; baseline and differential.
DR Genevisible; E9Q9R3; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009566; P:fertilization; IMP:UniProtKB.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IMP:UniProtKB.
DR InterPro; IPR038813; FIMP.
DR PANTHER; PTHR38648; PTHR38648; 1.
DR Pfam; PF17672; FIMP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Fertilization; Membrane;
KW Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..111
FT /note="Fertilization-influencing membrane protein"
FT /evidence="ECO:0000255"
FT /id="PRO_5003244526"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 76..92
FT /note="DSSPGLFQNILVGTLVV -> GTSRKGPNEISSQSQLR (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:32295885"
FT /id="VSP_060728"
FT VAR_SEQ 93..111
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:32295885"
FT /id="VSP_060729"
SQ SEQUENCE 111 AA; 12466 MW; 0B98DF82D643BCFF CRC64;
MKLWLWVAVG VWMLMAELGT IETAPRRDGT RPSVSGARPQ QVVNRLFFDY PDSDRASLLA
VARFIGEKPI TFVKTDSSPG LFQNILVGTL VVAFFFLLFQ FCLHVNFQKG A
