ID   FINO3_ECOLX             Reviewed;         186 AA.
AC   P29367;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Fertility inhibition protein;
DE   AltName: Full=Conjugal transfer repressor;
GN   Name=finO;
OS   Escherichia coli.
OG   Plasmid R6-5.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1916281; DOI=10.1016/0378-1119(91)90469-r;
RA   Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.;
RT   "Sequence and conservation of genes at the distal end of the transfer
RT   region on plasmids F and R6-5.";
RL   Gene 104:85-90(1991).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   PubMed=10876242; DOI=10.1038/76790;
RA   Ghetu A.F., Gubbins M.J., Frost L.S., Glover J.N.;
RT   "Crystal structure of the bacterial conjugation repressor finO.";
RL   Nat. Struct. Biol. 7:565-569(2000).
CC   -!- FUNCTION: One of the components on the FinOP fertility inhibition
CC       complex, which inhibits the expression of traJ gene, which in turn
CC       regulates the expression of some 20 transfer genes. The transfer genes
CC       are responsible for the process, called conjugal transfer, in which DNA
CC       is transmitted from one bacterial host to another. RNA-binding that
CC       interacts with the traJ mRNA and its antisense RNA, finP, stabilizing
CC       finP against endonucleolytic degradation and facilitating sense-
CC       antisense RNA recognition (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FinO family. {ECO:0000305}.
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DR   EMBL; M38048; AAA98316.1; -; Genomic_DNA.
DR   RefSeq; WP_000139323.1; NZ_WSWZ01000049.1.
DR   RefSeq; YP_002456228.1; NC_011812.1.
DR   RefSeq; YP_006952274.1; NC_019057.1.
DR   RefSeq; YP_006953358.1; NC_019071.1.
DR   RefSeq; YP_006953456.1; NC_019072.1.
DR   RefSeq; YP_006953983.1; NC_019090.1.
DR   RefSeq; YP_006954301.1; NC_019095.1.
DR   RefSeq; YP_007447581.1; NC_020278.2.
DR   PDB; 1DVO; X-ray; 2.00 A; A=33-184.
DR   PDBsum; 1DVO; -.
DR   AlphaFoldDB; P29367; -.
DR   SMR; P29367; -.
DR   EvolutionaryTrace; P29367; -.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1710.10; -; 1.
DR   InterPro; IPR021065; Fertility_inhibition_FinO_N.
DR   InterPro; IPR016103; ProQ/FinO.
DR   InterPro; IPR036442; ProQ/FinO_sf.
DR   Pfam; PF12602; FinO_N; 1.
DR   Pfam; PF04352; ProQ; 1.
DR   SMART; SM00945; ProQ; 1.
DR   SUPFAM; SSF48657; SSF48657; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Conjugation; Plasmid; Repressor; RNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..186
FT                   /note="Fertility inhibition protein"
FT                   /id="PRO_0000068352"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..21
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           35..64
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           65..68
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           73..80
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   TURN            81..83
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           85..87
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           100..110
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           117..128
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           131..136
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   STRAND          142..144
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   STRAND          150..153
FT                   /evidence="ECO:0007829|PDB:1DVO"
FT   HELIX           156..183
FT                   /evidence="ECO:0007829|PDB:1DVO"
SQ   SEQUENCE   186 AA;  21280 MW;  D263B2BE06E2805D CRC64;
     MTEQKRPVLT LKRKTEGETP TRSRKTIINV TTPPKWKVKK QKLAEKAARE AELTAKKAQA
     RQALSIYLNL PTLDEAVNTL KPWWPGLFDG DTPRLLACGI RDVLLEDVAQ RNIPLSHKKL
     RRALKAITRS ESYLCAMKAG ACRYDTEGYV TEHISQEEEV YAAERLDKIR RQNRIKAELQ
     AVLDEQ