ID   FINO_ECOLI              Reviewed;         186 AA.
AC   P22707;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Fertility inhibition protein;
DE   AltName: Full=Conjugal transfer repressor;
GN   Name=finO;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2164585; DOI=10.1016/0022-2836(90)90145-c;
RA   Yoshioka Y., Fujita Y., Ohtsubo E.;
RT   "Nucleotide sequence of the promoter-distal region of the tra operon of
RT   plasmid R100, including traI (DNA helicase I) and traD genes.";
RL   J. Mol. Biol. 214:39-53(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1916281; DOI=10.1016/0378-1119(91)90469-r;
RA   Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.;
RT   "Sequence and conservation of genes at the distal end of the transfer
RT   region on plasmids F and R6-5.";
RL   Gene 104:85-90(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 23-169.
RC   STRAIN=ECOR 71;
RX   PubMed=8807284; DOI=10.1093/genetics/143.3.1091;
RA   Boyd E.F., Hill C.W., Rich S.M., Hartl D.L.;
RT   "Mosaic structure of plasmids from natural populations of Escherichia
RT   coli.";
RL   Genetics 143:1091-1100(1996).
CC   -!- FUNCTION: One of the components on the FinOP fertility inhibition
CC       complex, which inhibits the expression of traJ gene, which in turn
CC       regulates the expression of some 20 transfer genes. The transfer genes
CC       are responsible for the process, called conjugal transfer, in which DNA
CC       is transmitted from one bacterial host to another. RNA-binding that
CC       interacts with the traJ mRNA and its antisense RNA, finP, stabilizing
CC       finP against endonucleolytic degradation and facilitating sense-
CC       antisense RNA recognition.
CC   -!- SIMILARITY: Belongs to the FinO family. {ECO:0000305}.
CC   -!- CAUTION: In strain K12 / CR63, finO is interrupted by an IS3 element.
CC       {ECO:0000305}.
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DR   EMBL; X57429; CAA40676.1; -; Genomic_DNA.
DR   EMBL; M38047; AAA98092.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44176.1; -; Genomic_DNA.
DR   EMBL; AP001918; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U50705; AAC44289.1; -; Genomic_DNA.
DR   PIR; JQ1339; JQ1339.
DR   AlphaFoldDB; P22707; -.
DR   SMR; P22707; -.
DR   PRIDE; P22707; -.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1710.10; -; 1.
DR   InterPro; IPR021065; Fertility_inhibition_FinO_N.
DR   InterPro; IPR016103; ProQ/FinO.
DR   InterPro; IPR036442; ProQ/FinO_sf.
DR   Pfam; PF12602; FinO_N; 1.
DR   Pfam; PF04352; ProQ; 1.
DR   SMART; SM00945; ProQ; 1.
DR   SUPFAM; SSF48657; SSF48657; 1.
PE   3: Inferred from homology;
KW   Conjugation; Plasmid; Repressor; RNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..186
FT                   /note="Fertility inhibition protein"
FT                   /id="PRO_0000068349"
FT   CONFLICT        132
FT                   /note="S -> SS (in Ref. 1; CAA40676)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   186 AA;  21222 MW;  F79605BBB87438D2 CRC64;
     MTEQKRPVLT LKRKTEGETL VRSRKTIINV TTPPKWKVKK QKLAEKAARE AELAAKKAQA
     RQALSIYLNL PTLDDAVNTL KPWWPGLFDG DTPRLLACGI RDVLLEDVAQ RNIPLSHKKL
     RRALKAITRS ESYLCAMKAG ACRYDTEGYV TEHISQEEEA YAAERLDKIR RQNRIKAELQ
     AVLDEK