ID   FIS1_TUBBO              Reviewed;         155 AA.
AC   Q6WRS2;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Mitochondrial fission 1 protein;
GN   Name=FIS1;
OS   Tuber borchii (White truffle).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC   Pezizales; Tuberaceae; Tuber.
OX   NCBI_TaxID=42251;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=ATCC 96540;
RX   PubMed=12910371; DOI=10.1007/s00294-003-0430-3;
RA   Guidi C., Zeppa S., Barbieri E., Zambonelli A., Polidori E., Potenza L.,
RA   Stocchi V.;
RT   "A putative mitochondrial fission gene from the ectomycorrhizal ascomycete
RT   Tuber borchii Vittad.: cloning, characterisation and phylogeny.";
RL   Curr. Genet. 44:148-154(2003).
CC   -!- FUNCTION: Has a role in mitochondrial fission. Has a role in outer
CC       membrane fission but not matrix separation (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed during fruit body ripening.
CC       Levels increase during fruit body stages 1, 2 and 3, the stages of
CC       ascospore formation and maturation. Levels decrease during mycelium
CC       aging. {ECO:0000269|PubMed:12910371}.
CC   -!- DOMAIN: The C-terminus is required for mitochondrial localization,
CC       while the N-terminus is necessary for mitochondrial fission.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FIS1 family. {ECO:0000305}.
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DR   EMBL; AY273171; AAQ17209.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6WRS2; -.
DR   SMR; Q6WRS2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000266; P:mitochondrial fission; IEA:InterPro.
DR   CDD; cd12212; Fis1; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR016543; Fis1.
DR   InterPro; IPR033745; Fis1_cytosol.
DR   InterPro; IPR028061; Fis1_TPR_C.
DR   InterPro; IPR028058; Fis1_TPR_N.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR13247; PTHR13247; 1.
DR   Pfam; PF14853; Fis1_TPR_C; 1.
DR   Pfam; PF14852; Fis1_TPR_N; 1.
DR   PIRSF; PIRSF008835; TPR_repeat_11_Fis1; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion outer membrane; Repeat; TPR repeat;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..155
FT                   /note="Mitochondrial fission 1 protein"
FT                   /id="PRO_0000256190"
FT   TOPO_DOM        1..127
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        149..155
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REPEAT          75..108
FT                   /note="TPR"
SQ   SEQUENCE   155 AA;  17596 MW;  574FDB09F2E9D112 CRC64;
     MPSDNLPHAV DAESPLKEAE LQVLRNQYEK EGQFVGVQTK FNYAWGLIKS DKRPEQQMGV
     RLLTDIFRDH TERRRECLYY LALGNYKLGN YAEARRYNDR LLENEPANLQ SQSLRGLIDD
     KVAKEGMLGV AIISGVALAA GLVGSALWRG MSRRR