FIS2C_ARATH
ID FIS2C_ARATH Reviewed; 755 AA.
AC P0DKJ8; Q0QMM9; Q9ZNT9; Q9ZQP0;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=Polycomb group protein FERTILIZATION-INDEPENDENT SEED 2 {ECO:0000303|PubMed:10962025};
GN Name=FIS2 {ECO:0000303|PubMed:10962025};
GN OrderedLocusNames=At2g35670 {ECO:0000312|Araport:AT2G35670};
GN ORFNames=T20F21.14 {ECO:0000312|EMBL:AAD15448.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10962025; DOI=10.1073/pnas.170292997;
RA Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.;
RT "Expression and parent-of-origin effects for FIS2, MEA, and FIE in the
RT endosperm and embryo of developing Arabidopsis seeds.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000).
RN [4]
RP FUNCTION.
RX PubMed=15151989; DOI=10.1242/dev.01168;
RA Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E.,
RA Grossniklaus U., Berger F.;
RT "Identification of new members of fertilisation independent seed Polycomb
RT group pathway involved in the control of seed development in Arabidopsis
RT thaliana.";
RL Development 131:2971-2981(2004).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They probably act
CC via the methylation of histones, rendering chromatin heritably changed
CC in its expressibility. Required to prevent the proliferation of the
CC central cell by repressing unknown target genes before fertilization.
CC Regulates the anteroposterior organization of the endosperm.
CC {ECO:0000269|PubMed:15151989}.
CC -!- SUBUNIT: Probably indirectly associated with FIE and/or MEA. In plants,
CC PcG complexes are probably composed of a member of the EZ family (CLF
CC or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2).
CC {ECO:0000250|UniProtKB:Q8W5B1}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10962025}.
CC -!- TISSUE SPECIFICITY: Weakly expressed. Expressed in late siliques.
CC {ECO:0000269|PubMed:10962025}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically. Expressed in
CC the central cell before fertilization, and in the endosperm after
CC fertilization, then decreases before the time of endosperm
CC cellularization but continues in the chalazal cyst.
CC {ECO:0000269|PubMed:10962025}.
CC -!- SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
CC {ECO:0000305}.
CC -!- CAUTION: In cv. Landsberg erecta (AC P0DKJ7), the sequence differs from
CC that shown due to an insertion in the genomic sequence that adds 60
CC residues after the Asn-564. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD15448.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006068; AAD15448.2; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09143.1; -; Genomic_DNA.
DR PIR; E84771; E84771.
DR RefSeq; NP_565815.2; NM_129122.3.
DR AlphaFoldDB; P0DKJ8; -.
DR STRING; 3702.AT2G35670.1; -.
DR iPTMnet; P0DKJ8; -.
DR PaxDb; P0DKJ8; -.
DR PRIDE; P0DKJ8; -.
DR EnsemblPlants; AT2G35670.1; AT2G35670.1; AT2G35670.
DR GeneID; 818136; -.
DR Gramene; AT2G35670.1; AT2G35670.1; AT2G35670.
DR KEGG; ath:AT2G35670; -.
DR Araport; AT2G35670; -.
DR eggNOG; KOG2350; Eukaryota.
DR OrthoDB; 516359at2759; -.
DR PhylomeDB; P0DKJ8; -.
DR PRO; PR:P0DKJ8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P0DKJ8; baseline and differential.
DR Genevisible; Q9ZNT9; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR019135; Polycomb_protein_VEFS-Box.
DR Pfam; PF09733; VEFS-Box; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..755
FT /note="Polycomb group protein FERTILIZATION-INDEPENDENT
FT SEED 2"
FT /id="PRO_0000047836"
FT REPEAT 245..266
FT /note="A-1"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 267..283
FT /note="B-1"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 284..306
FT /note="A-2"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 307..329
FT /note="A-3"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 330..351
FT /note="A-4"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 352..373
FT /note="A-5"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 374..390
FT /note="B-2"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 405..421
FT /note="B-3"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 422..443
FT /note="A-6"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 444..460
FT /note="B-4"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 461..483
FT /note="A-7"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 484..504
FT /note="A-8"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 505..521
FT /note="B-5"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 522..544
FT /note="A-9"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 545..565
FT /note="A-10"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REPEAT 566..582
FT /note="B-6"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT ZN_FING 136..157
FT /note="C2H2-type"
FT /evidence="ECO:0000255"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..565
FT /note="10 X approximate repeat A"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REGION 267..582
FT /note="6 X approximate repeat B"
FT /evidence="ECO:0000250|UniProtKB:P0DKJ7"
FT REGION 276..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 590..725
FT /note="VEFS-box"
FT /evidence="ECO:0000255"
FT COMPBIAS 245..263
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..331
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..363
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 364..382
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 461..475
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 485..515
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 552..571
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 755 AA; 85396 MW; 8A0A75A3043F5BE4 CRC64;
MARKSIRGKE VVMVSDDDDD DDDDVDDDKN IIKCVKPLTV YKNLETPTDS DDNDDDDDDD
VDVDENIIKY IKPVAVYKKL ETRSKNNPYF LRRSLKYIIQ AKKKKKSNSG GKIRFNYRDV
SNKMTLKAEV VENFSCPFCL IPCGGHEGLQ LHLKSSHDAF KFEFYRAEKD HGPEVDVSVK
SDTIKFGVLK DDVGNPQLSP LTFCSKNRNQ RRQRDDSNNV KKLNVLLMEL DLDDLPRGTE
NDSTHVNDDN VSSPPRAHSS EKISDILTTT QLAIAESSEP KVPHVNDGNV SSPPRAHSSA
EKNESTHVND DDDVSSPPRA HSLEKNESTH VNEDNISSPP KAHSSKKNES THMNDEDVSF
PPRTRSSKET SDILTTTQPA IVEPSEPKVR RVSRRKQLYA KRYKARETQP AIAESSEPKV
LHVNDENVSS PPEAHSLEKA SDILTTTQPA IAESSEPKVP HVNDENVSST PRAHSSKKNK
STRKNVDNVP SPPKTRSSKK TSDILTTTQP TIAESSEPKV RHVNDDNVSS TPRAHSSKKN
KSTRKNDDNI PSPPKTRSSK KTSNILATTQ PAKAEPSEPK VTRVSRRKEL HAERCEAKRL
ERLKGRQFYH SQTMQPITFE QVMSNEDSEN ETDDYALDIS ERLRLERLVG VSKEEKRYMY
LWNIFVRKQR VIADGHVPWA CEEFAKLHKE EMKNSSSFDW WWRMFRIKLW NNGLICAKTF
HKCTTILLSN SDEAGQFTSG SAANANNQQS MEVDE
