FIS2L_ARATH
ID FIS2L_ARATH Reviewed; 813 AA.
AC P0DKJ7; Q0QMM9; Q9ZNT9; Q9ZQP0;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=Polycomb group protein FERTILIZATION-INDEPENDENT SEED 2 {ECO:0000303|PubMed:16924116};
GN Name=FIS2 {ECO:0000303|PubMed:16924116};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=16924116; DOI=10.1073/pnas.0605551103;
RA Wang D., Tyson M.D., Jackson S.S., Yadegari R.;
RT "Partially redundant functions of two SET-domain polycomb-group proteins in
RT controlling initiation of seed development in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:13244-13249(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 87-813, AND MUTAGENESIS OF
RP 758-TRP--GLU-813.
RC STRAIN=cv. Landsberg erecta; TISSUE=Silique;
RX PubMed=9874812; DOI=10.1073/pnas.96.1.296;
RA Luo M., Bilodeau P., Koltunow A., Dennis E.S., Peacock W.J., Chaudhury A.;
RT "Genes controlling fertilization-independent seed development in
RT Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:296-301(1999).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10962025; DOI=10.1073/pnas.170292997;
RA Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.;
RT "Expression and parent-of-origin effects for FIS2, MEA, and FIE in the
RT endosperm and embryo of developing Arabidopsis seeds.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000).
RN [4]
RP FUNCTION.
RX PubMed=15151989; DOI=10.1242/dev.01168;
RA Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E.,
RA Grossniklaus U., Berger F.;
RT "Identification of new members of fertilisation independent seed Polycomb
RT group pathway involved in the control of seed development in Arabidopsis
RT thaliana.";
RL Development 131:2971-2981(2004).
CC -!- FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming
CC multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development. PcG proteins are not required to initiate repression, but
CC to maintain it during later stages of development. They probably act
CC via the methylation of histones, rendering chromatin heritably changed
CC in its expressibility. Required to prevent the proliferation of the
CC central cell by repressing unknown target genes before fertilization.
CC Regulates the anteroposterior organization of the endosperm.
CC {ECO:0000269|PubMed:15151989}.
CC -!- SUBUNIT: Probably indirectly associated with FIE and/or MEA. In plants,
CC PcG complexes are probably composed of a member of the EZ family (CLF
CC or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2).
CC {ECO:0000250|UniProtKB:Q8W5B1}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10962025}.
CC -!- TISSUE SPECIFICITY: Weakly expressed. Expressed in late siliques.
CC {ECO:0000269|PubMed:10962025}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically. Expressed in
CC the central cell before fertilization, and in the endosperm after
CC fertilization, then decreases before the time of endosperm
CC cellularization but continues in the chalazal cyst.
CC {ECO:0000269|PubMed:10962025}.
CC -!- SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
CC {ECO:0000305}.
CC -!- CAUTION: In cv. Columbia (AC P0DKJ8), the sequence differs from that
CC shown due to a deletion in the genomic sequence that remove 60 residues
CC after the Asn-562. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD09104.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAD09105.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ275158; ABB84250.1; -; mRNA.
DR EMBL; AF096095; AAD09104.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF096096; AAD09105.1; ALT_INIT; mRNA.
DR PIR; E84771; E84771.
DR AlphaFoldDB; P0DKJ7; -.
DR PRIDE; P0DKJ7; -.
DR ExpressionAtlas; P0DKJ7; baseline and differential.
DR Genevisible; Q9ZNT9; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR019135; Polycomb_protein_VEFS-Box.
DR Pfam; PF09733; VEFS-Box; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Metal-binding; Nucleus; Repeat; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..813
FT /note="Polycomb group protein FERTILIZATION-INDEPENDENT
FT SEED 2"
FT /id="PRO_0000435364"
FT REPEAT 243..264
FT /note="A-1"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 265..281
FT /note="B-1"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 282..304
FT /note="A-2"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 305..327
FT /note="A-3"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 328..349
FT /note="A-4"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 350..371
FT /note="A-5"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 372..388
FT /note="B-2"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 403..419
FT /note="B-3"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 420..441
FT /note="A-6"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 442..458
FT /note="B-4"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 459..481
FT /note="A-7"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 482..502
FT /note="A-8"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 503..519
FT /note="B-5"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 520..542
FT /note="A-9"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 543..563
FT /note="A-10"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 564..579
FT /note="B-6"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 580..601
FT /note="A-11"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 602..623
FT /note="A-12"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REPEAT 624..640
FT /note="B-7"
FT /evidence="ECO:0000269|PubMed:9874812"
FT ZN_FING 134..155
FT /note="C2H2-type"
FT /evidence="ECO:0000255"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 243..542
FT /note="12 X approximate repeat A"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REGION 265..640
FT /note="7 X approximate repeat B"
FT /evidence="ECO:0000269|PubMed:9874812"
FT REGION 274..648
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 648..783
FT /note="VEFS-box"
FT /evidence="ECO:0000255"
FT COMPBIAS 243..261
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 343..361
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..473
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..513
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 550..569
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 577..591
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 613..628
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 758..813
FT /note="Missing: In fis2-4; induces atrophy of partially
FT developing seeds beyond the globular stage."
FT /evidence="ECO:0000269|PubMed:9874812"
FT CONFLICT 533
FT /note="H -> N (in Ref. 1; ABB84250)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 813 AA; 91702 MW; 2A38DDB3F99232BE CRC64;
MARKSIRGKE VVMVSDDDDD DDDVDDDKNI IKCVKPLTVY KNLETPTDSD DNDDDDDDVD
VDENIIKYIK PVAVYKKLET RSKNNPYFLR RSLKYIIQAK KKKKSNSGGK IRFNYRDVSN
KMTLKAEVVE NFSCPFCLIP CGGHEGLQLH LKSSHDAFKF EFYRAEKDHG PEVDVSVKSD
TIKFGVLKDD VGNPQLSPLT FCSKNRNQRR QRDDSNNVKK LNVLLMELDL DDLPRGTEND
STHVNDDNVS SPPRAHSSEK ISDILTTTQL AIAESSEPKV PHVNDGNVSS PPRAHSSAEK
NESTHVNDDD DVSSPPRAHS LEKNESTHVN EDNISSPPKA HSSKKNESTH MNDEDVSFPP
RTRSSKETSD ILTTTQPAIV EPSEPKVRRG SRRKQLYAKR YKARETQPAI AESSEPKVLH
VNDENVSSPP EAHSLEKASD ILTTTQPAIA ESSEPKVPHV NDENVSSTPR AHSSKKNKST
RKNVDNVPSP PKTRSSKKTS DILTTTQPTI AESSEPKVRH VNDDNVSSTP RAHSSKKNKS
TRKNDDNIPS PPKTRSSKKT SNILTRTQPA IAESEPKVPH VNDDKVSSTP RAHSSKKNKS
THKKDDNASL PPKTRSSKKT SDILATTQPA KAEPSEPKVT RVSRRKELHA ERCEAKRLER
LKGRQFYHSQ TMQPMTFEQV MSNEDSENET DDYALDISER LRLERLVGVS KEEKRYMYLW
NIFVRKQRVI ADGHVPWACE EFAKLHKEEM KNSSSFDWWW RMFRIKLWNN GLICAKTFHK
CTTILLSNSD EAGQFTSGSA ANANNQQSME VDE
