AKIP1_HUMAN
ID AKIP1_HUMAN Reviewed; 210 AA.
AC Q9NQ31; Q8NBS2; Q8TAC6; Q8TAD3; Q8TAE0;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=A-kinase-interacting protein 1;
DE AltName: Full=Breast cancer-associated gene 3 protein;
DE AltName: Full=PKA-interacting protein;
DE AltName: Full=Proline-rich protein BCA3;
GN Name=AKIP1; Synonyms=BCA3, C11orf17;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RC TISSUE=Lymph node;
RX PubMed=12527432; DOI=10.1016/s0167-4781(02)00562-6;
RA Kitching R., Li H., Wong M.J., Kanaganayakam S., Kahn H., Seth A.;
RT "Characterization of a novel human breast cancer associated gene (BCA3)
RT encoding an alternatively spliced proline-rich protein.";
RL Biochim. Biophys. Acta 1625:116-121(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RX PubMed=11528127; DOI=10.1159/000056999;
RA Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A.,
RA Zabel B., Hankeln T., Schmidt E.R.;
RT "Comparative genomic sequencing reveals a strikingly similar architecture
RT of a conserved syntenic region on human chromosome 11p15.3 (including gene
RT ST5) and mouse chromosome 7.";
RL Cytogenet. Cell Genet. 93:284-290(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT LYS-23.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT LYS-23.
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION WITH PRKACA.
RX PubMed=15630084; DOI=10.1073/pnas.0408608102;
RA Sastri M., Barraclough D.M., Carmichael P.T., Taylor S.S.;
RT "A-kinase-interacting protein localizes protein kinase A in the nucleus.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:349-354(2005).
RN [6]
RP FUNCTION, INTERACTION WITH RELA, AND SUBCELLULAR LOCATION.
RX PubMed=18178962; DOI=10.1074/jbc.m710285200;
RA Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.;
RT "AKIP1 enhances NF-kappaB-dependent gene expression by promoting the
RT nuclear retention and phosphorylation of p65.";
RL J. Biol. Chem. 283:7834-7843(2008).
RN [7]
RP FUNCTION.
RX PubMed=20562110; DOI=10.1074/jbc.m110.116566;
RA Gao N., Hibi Y., Cueno M., Asamitsu K., Okamoto T.;
RT "A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of
RT PKA in NF-kappaB signaling.";
RL J. Biol. Chem. 285:28097-28104(2010).
CC -!- FUNCTION: Enhances NF-kappa-B transcriptional activity by regulating
CC the nuclear localization of the NF-kappa-B subunit RELA and promoting
CC the phosphorylation of RELA by PRKACA. Regulates the effect of the
CC cAMP-dependent protein kinase signaling pathway on the NF-kappa-B
CC activation cascade. {ECO:0000269|PubMed:18178962,
CC ECO:0000269|PubMed:20562110}.
CC -!- SUBUNIT: Interacts with PRKACA and RELA. {ECO:0000269|PubMed:15630084,
CC ECO:0000269|PubMed:18178962}.
CC -!- INTERACTION:
CC Q9NQ31; P22607: FGFR3; NbExp=3; IntAct=EBI-517035, EBI-348399;
CC Q9NQ31; Q14192: FHL2; NbExp=3; IntAct=EBI-517035, EBI-701903;
CC Q9NQ31; P06396: GSN; NbExp=3; IntAct=EBI-517035, EBI-351506;
CC Q9NQ31; P17612: PRKACA; NbExp=4; IntAct=EBI-517035, EBI-476586;
CC Q9NQ31; Q9Y649; NbExp=3; IntAct=EBI-517035, EBI-25900580;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15630084,
CC ECO:0000269|PubMed:18178962}. Note=Locates to punctate spots.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=1a;
CC IsoId=Q9NQ31-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NQ31-2; Sequence=VSP_013276;
CC Name=3; Synonyms=1b;
CC IsoId=Q9NQ31-3; Sequence=VSP_013275;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in adult heart and at
CC lower levels in brain, testis, ovary and skeletal muscle. Up-regulated
CC in some breast cancer cell lines. Isoform 1 and isoform 3 are expressed
CC in fetal brain. {ECO:0000269|PubMed:12527432,
CC ECO:0000269|PubMed:15630084}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF493783; AAM12862.1; -; mRNA.
DR EMBL; AF493784; AAM12863.1; -; mRNA.
DR EMBL; AF493785; AAM12864.1; -; mRNA.
DR EMBL; AF493786; AAM12865.1; -; Genomic_DNA.
DR EMBL; AF493786; AAM12866.1; -; Genomic_DNA.
DR EMBL; AF493786; AAM12867.1; -; Genomic_DNA.
DR EMBL; AF512007; AAM34785.1; -; mRNA.
DR EMBL; AJ400877; CAB92290.1; -; Genomic_DNA.
DR EMBL; AK075308; BAC11537.1; -; mRNA.
DR EMBL; BC030996; AAH30996.1; -; mRNA.
DR CCDS; CCDS55743.1; -. [Q9NQ31-2]
DR CCDS; CCDS55744.1; -. [Q9NQ31-3]
DR CCDS; CCDS7793.1; -. [Q9NQ31-1]
DR RefSeq; NP_001193575.1; NM_001206646.1. [Q9NQ31-3]
DR RefSeq; NP_001193576.1; NM_001206647.1. [Q9NQ31-2]
DR RefSeq; NP_001193577.1; NM_001206648.1.
DR RefSeq; NP_065693.2; NM_020642.3. [Q9NQ31-1]
DR RefSeq; XP_016873500.1; XM_017018011.1. [Q9NQ31-1]
DR AlphaFoldDB; Q9NQ31; -.
DR BioGRID; 121180; 21.
DR IntAct; Q9NQ31; 13.
DR MINT; Q9NQ31; -.
DR STRING; 9606.ENSP00000310459; -.
DR iPTMnet; Q9NQ31; -.
DR PhosphoSitePlus; Q9NQ31; -.
DR BioMuta; AKIP1; -.
DR EPD; Q9NQ31; -.
DR MassIVE; Q9NQ31; -.
DR MaxQB; Q9NQ31; -.
DR PaxDb; Q9NQ31; -.
DR PeptideAtlas; Q9NQ31; -.
DR PRIDE; Q9NQ31; -.
DR ProteomicsDB; 82063; -. [Q9NQ31-1]
DR ProteomicsDB; 82064; -. [Q9NQ31-2]
DR ProteomicsDB; 82065; -. [Q9NQ31-3]
DR Antibodypedia; 24164; 241 antibodies from 26 providers.
DR DNASU; 56672; -.
DR Ensembl; ENST00000299576.9; ENSP00000299576.5; ENSG00000166452.12. [Q9NQ31-3]
DR Ensembl; ENST00000309357.8; ENSP00000310644.4; ENSG00000166452.12. [Q9NQ31-2]
DR Ensembl; ENST00000309377.9; ENSP00000310459.4; ENSG00000166452.12. [Q9NQ31-1]
DR Ensembl; ENST00000396648.6; ENSP00000379885.2; ENSG00000166452.12. [Q9NQ31-3]
DR Ensembl; ENST00000534147.5; ENSP00000431331.1; ENSG00000166452.12. [Q9NQ31-1]
DR GeneID; 56672; -.
DR KEGG; hsa:56672; -.
DR MANE-Select; ENST00000309377.9; ENSP00000310459.4; NM_020642.4; NP_065693.2.
DR UCSC; uc001mgx.4; human. [Q9NQ31-1]
DR CTD; 56672; -.
DR DisGeNET; 56672; -.
DR GeneCards; AKIP1; -.
DR HGNC; HGNC:1170; AKIP1.
DR HPA; ENSG00000166452; Low tissue specificity.
DR MIM; 609191; gene.
DR neXtProt; NX_Q9NQ31; -.
DR OpenTargets; ENSG00000166452; -.
DR PharmGKB; PA25484; -.
DR VEuPathDB; HostDB:ENSG00000166452; -.
DR eggNOG; ENOG502S6M5; Eukaryota.
DR GeneTree; ENSGT00390000017064; -.
DR HOGENOM; CLU_126629_1_0_1; -.
DR InParanoid; Q9NQ31; -.
DR OMA; DYASSQC; -.
DR OrthoDB; 1398399at2759; -.
DR PhylomeDB; Q9NQ31; -.
DR TreeFam; TF337318; -.
DR PathwayCommons; Q9NQ31; -.
DR SignaLink; Q9NQ31; -.
DR BioGRID-ORCS; 56672; 18 hits in 1081 CRISPR screens.
DR ChiTaRS; AKIP1; human.
DR GenomeRNAi; 56672; -.
DR Pharos; Q9NQ31; Tbio.
DR PRO; PR:Q9NQ31; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9NQ31; protein.
DR Bgee; ENSG00000166452; Expressed in oocyte and 176 other tissues.
DR ExpressionAtlas; Q9NQ31; baseline and differential.
DR Genevisible; Q9NQ31; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:1901222; P:regulation of NIK/NF-kappaB signaling; IEA:InterPro.
DR GO; GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Ensembl.
DR InterPro; IPR033214; AKIP1.
DR PANTHER; PTHR14330; PTHR14330; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Proto-oncogene; Reference proteome.
FT CHAIN 1..210
FT /note="A-kinase-interacting protein 1"
FT /id="PRO_0000064857"
FT REGION 58..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..161
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 75..101
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12527432"
FT /id="VSP_013275"
FT VAR_SEQ 137..163
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12527432"
FT /id="VSP_013276"
FT VARIANT 23
FT /note="R -> K (in dbSNP:rs1133833)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_021565"
FT VARIANT 132
FT /note="I -> T (in dbSNP:rs35131475)"
FT /id="VAR_050688"
FT CONFLICT 41
FT /note="A -> T (in Ref. 4; BAC11537)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="F -> S (in Ref. 4; BAC11537)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 210 AA; 23114 MW; F00AA1F166C37967 CRC64;
MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE
KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC GKYYSSVPEE GGATHVYRYH
RGESKLHMCL DIGNGQRKDR KKTSLGPGGS YQISEHAPEA SQPAENISKD LYIEVYPGTY
SVTVGSNDLT KKTHVVAVDS GQSVDLVFPV
