FIT_ARATH
ID FIT_ARATH Reviewed; 318 AA.
AC Q0V7X4; Q8S3E9; Q9ZUV8;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR;
DE AltName: Full=Basic helix-loop-helix protein 29;
DE Short=AtbHLH29;
DE Short=bHLH 29;
DE AltName: Full=FER-LIKE REGULATOR OF IRON UPTAKE;
DE AltName: Full=Transcription factor EN 43;
DE AltName: Full=Transcription factor Fe-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1;
DE AltName: Full=bHLH transcription factor bHLH029;
GN Name=FIT; Synonyms=BHLH29, EN43, FIT1, FRU; OrderedLocusNames=At2g28160;
GN ORFNames=F24D13.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY IRON DEFICIENCY.
RX PubMed=15556641; DOI=10.1016/j.febslet.2004.10.062;
RA Jakoby M., Wang H.-Y., Reidt W., Weisshaar B., Bauer P.;
RT "FRU (BHLH029) is required for induction of iron mobilization genes in
RT Arabidopsis thaliana.";
RL FEBS Lett. 577:528-534(2004).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY IRON DEFICIENCY.
RX PubMed=15539473; DOI=10.1105/tpc.104.024315;
RA Colangelo E.P., Guerinot M.L.;
RT "The essential basic helix-loop-helix protein FIT1 is required for the iron
RT deficiency response.";
RL Plant Cell 16:3400-3412(2004).
RN [9]
RP REVIEW, AND INDUCTION BY IRON DEFICIENCY.
RX PubMed=15531708; DOI=10.1104/pp.104.047233;
RA Bauer P., Thiel T., Klatte M., Bereczky Z., Brumbarova T., Hell R.,
RA Grosse I.;
RT "Analysis of sequence, map position, and gene expression reveals conserved
RT essential genes for iron uptake in Arabidopsis and tomato.";
RL Plant Physiol. 136:4169-4183(2004).
RN [10]
RP FUNCTION.
RX PubMed=16117851; DOI=10.1038/sj.cr.7290331;
RA Yuan Y.X., Zhang J., Wang D.W., Ling H.Q.;
RT "AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER
RT involved in controlling iron acquisition in strategy I plants.";
RL Cell Res. 15:613-621(2005).
RN [11]
RP NOMENCLATURE.
RX PubMed=17466530; DOI=10.1016/j.plaphy.2007.03.006;
RA Bauer P., Ling H.-Q., Guerinot M.L.;
RT "FIT, the FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR in
RT Arabidopsis.";
RL Plant Physiol. Biochem. 45:260-261(2007).
RN [12]
RP INDUCTION BY CYTOKININS.
RX PubMed=18397377; DOI=10.1111/j.1365-313x.2008.03502.x;
RA Seguela M., Briat J.-F., Vert G., Curie C.;
RT "Cytokinins negatively regulate the root iron uptake machinery in
RT Arabidopsis through a growth-dependent pathway.";
RL Plant J. 55:289-300(2008).
CC -!- FUNCTION: Transcription factor. Essential protein involved in iron
CC uptake responses. Regulates FRO2 at the level of mRNA accumulation and
CC IRT1 at the level of protein accumulation. Confers enhanced iron
CC mobilization responses at low iron supply.
CC {ECO:0000269|PubMed:15539473, ECO:0000269|PubMed:15556641,
CC ECO:0000269|PubMed:16117851}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q0V7X4; Q9SI38: ANR1; NbExp=3; IntAct=EBI-1640543, EBI-622096;
CC Q0V7X4; Q9ZVB5: BHLH100; NbExp=3; IntAct=EBI-1640543, EBI-15198769;
CC Q0V7X4; Q9FYE6: BHLH101; NbExp=4; IntAct=EBI-1640543, EBI-15195485;
CC Q0V7X4; Q1PF17: BHLH18; NbExp=3; IntAct=EBI-1640543, EBI-15195549;
CC Q0V7X4; Q56XR0: BHLH71; NbExp=3; IntAct=EBI-1640543, EBI-4432361;
CC Q0V7X4; Q9M1K1: ORG2; NbExp=5; IntAct=EBI-1640543, EBI-1640553;
CC Q0V7X4; Q9M1K0: ORG3; NbExp=5; IntAct=EBI-1640543, EBI-1640573;
CC Q0V7X4; F4JCN9: PRE4; NbExp=4; IntAct=EBI-1640543, EBI-15192623;
CC Q0V7X4; Q93ZE2: TGA7; NbExp=3; IntAct=EBI-1640543, EBI-541400;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and inflorescence, and to a
CC lower extent, in leaves and stems. In roots, confined to the outer cell
CC layers, specifically in the differentiation zone. Also detected in the
CC endodermis and inner tissues of the central cylinder.
CC {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:15539473,
CC ECO:0000269|PubMed:15556641}.
CC -!- INDUCTION: In roots by iron deficiency. Repressed by cytokinins.
CC Induced by cold, UV, ethylene (ACC), jasmonic acid (JA), flagellin, and
CC salicylic acid (SA) treatments. {ECO:0000269|PubMed:12679534,
CC ECO:0000269|PubMed:15531708, ECO:0000269|PubMed:15539473,
CC ECO:0000269|PubMed:15556641, ECO:0000269|PubMed:18397377}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC98450.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF488570; AAM10938.1; -; mRNA.
DR EMBL; AC005851; AAC98450.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08086.1; -; Genomic_DNA.
DR EMBL; BT026446; ABH04553.1; -; mRNA.
DR PIR; E84681; E84681.
DR RefSeq; NP_850114.1; NM_179783.2.
DR AlphaFoldDB; Q0V7X4; -.
DR SMR; Q0V7X4; -.
DR BioGRID; 2712; 28.
DR IntAct; Q0V7X4; 24.
DR STRING; 3702.AT2G28160.1; -.
DR PaxDb; Q0V7X4; -.
DR PRIDE; Q0V7X4; -.
DR EnsemblPlants; AT2G28160.1; AT2G28160.1; AT2G28160.
DR GeneID; 817362; -.
DR Gramene; AT2G28160.1; AT2G28160.1; AT2G28160.
DR KEGG; ath:AT2G28160; -.
DR Araport; AT2G28160; -.
DR TAIR; locus:2046198; AT2G28160.
DR eggNOG; ENOG502QSJP; Eukaryota.
DR HOGENOM; CLU_864384_0_0_1; -.
DR InParanoid; Q0V7X4; -.
DR OMA; CHEVDIN; -.
DR OrthoDB; 1155429at2759; -.
DR PhylomeDB; Q0V7X4; -.
DR PRO; PR:Q0V7X4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0V7X4; baseline and differential.
DR Genevisible; Q0V7X4; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034756; P:regulation of iron ion transport; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010039; P:response to iron ion; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..318
FT /note="Transcription factor FER-LIKE IRON DEFICIENCY-
FT INDUCED TRANSCRIPTION FACTOR"
FT /id="PRO_0000358844"
FT DOMAIN 127..176
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 90..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 55
FT /note="S -> C (in Ref. 1; AAM10938)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="M -> V (in Ref. 1; AAM10938)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="K -> E (in Ref. 1; AAM10938)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="K -> R (in Ref. 1; AAM10938)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 318 AA; 35520 MW; 153B7D71E11A7619 CRC64;
MEGRVNALSN INDLELHNFL VDPNFDQFIN LIRGDHQTID ENPVLDFDLG PLQNSPCFID
ENQFIPTPVD DLFDELPDLD SNVAESFRSF DGDSVRAGGE EDEEDYNDGD DSSATTTNND
GTRKTKTDRS RTLISERRRR GRMKDKLYAL RSLVPNITKM DKASIVGDAV LYVQELQSQA
KKLKSDIAGL EASLNSTGGY QEHAPDAQKT QPFRGINPPA SKKIIQMDVI QVEEKGFYVR
LVCNKGEGVA PSLYKSLESL TSFQVQNSNL SSPSPDTYLL TYTLDGTCFE QSLNLPNLKL
WITGSLLNQG FEFIKSFT
