ID   FIXA_ECOLI              Reviewed;         256 AA.
AC   P60566; P31573; P75625; P76901; Q8XA29;
DT   15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2004, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Protein FixA;
GN   Name=fixA; Synonyms=yaaQ; OrderedLocusNames=b0041, JW0040;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O44:K74;
RX   PubMed=7473063; DOI=10.1002/jobm.3620350404;
RA   Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A.;
RT   "The fix Escherichia coli region contains four genes related to carnitine
RT   metabolism.";
RL   J. Basic Microbiol. 35:217-227(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA   Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA   Mizobuchi K., Nakata A.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT   2.4 min region.";
RL   Nucleic Acids Res. 20:3305-3308(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 80.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION.
RC   STRAIN=K12 / BW25113;
RX   PubMed=12081978; DOI=10.1128/jb.184.14.4044-4047.2002;
RA   Walt A., Kahn M.L.;
RT   "The fixA and fixB genes are necessary for anaerobic carnitine reduction in
RT   Escherichia coli.";
RL   J. Bacteriol. 184:4044-4047(2002).
CC   -!- FUNCTION: Required for anaerobic carnitine reduction. May bring
CC       reductant to CaiA. {ECO:0000269|PubMed:12081978}.
CC   -!- PATHWAY: Amine and polyamine metabolism; carnitine metabolism.
CC   -!- SUBUNIT: Heterodimer of FixA and FixB. {ECO:0000305}.
CC   -!- INTERACTION:
CC       P60566; P0A9U1: ybhF; NbExp=3; IntAct=EBI-1113126, EBI-547696;
CC   -!- SIMILARITY: Belongs to the ETF beta-subunit/FixA family. {ECO:0000305}.
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DR   EMBL; X71977; CAA50797.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73152.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAB96610.2; -; Genomic_DNA.
DR   PIR; A64725; A64725.
DR   RefSeq; NP_414583.2; NC_000913.3.
DR   RefSeq; WP_000692204.1; NZ_STEB01000010.1.
DR   AlphaFoldDB; P60566; -.
DR   SMR; P60566; -.
DR   BioGRID; 4262207; 10.
DR   BioGRID; 851643; 3.
DR   IntAct; P60566; 8.
DR   STRING; 511145.b0041; -.
DR   PaxDb; P60566; -.
DR   PRIDE; P60566; -.
DR   EnsemblBacteria; AAC73152; AAC73152; b0041.
DR   EnsemblBacteria; BAB96610; BAB96610; BAB96610.
DR   GeneID; 58460821; -.
DR   GeneID; 947316; -.
DR   KEGG; ecj:JW0040; -.
DR   KEGG; eco:b0041; -.
DR   PATRIC; fig|1411691.4.peg.2242; -.
DR   EchoBASE; EB1523; -.
DR   eggNOG; COG2086; Bacteria.
DR   HOGENOM; CLU_060196_2_2_6; -.
DR   InParanoid; P60566; -.
DR   OMA; ADLNEWD; -.
DR   PhylomeDB; P60566; -.
DR   BioCyc; EcoCyc:EG11562-MON; -.
DR   UniPathway; UPA00117; -.
DR   PRO; PR:P60566; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0042413; P:carnitine catabolic process; IMP:EcoCyc.
DR   GO; GO:0009437; P:carnitine metabolic process; IMP:EcoliWiki.
DR   CDD; cd01714; ETF_beta; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_01055; FixA; 1.
DR   InterPro; IPR000049; ET-Flavoprotein_bsu_CS.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR012255; ETF_b.
DR   InterPro; IPR033948; ETF_beta_N.
DR   InterPro; IPR023463; FixA.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR21294; PTHR21294; 1.
DR   Pfam; PF01012; ETF; 1.
DR   PIRSF; PIRSF000090; Beta-ETF; 1.
DR   SMART; SM00893; ETF; 1.
DR   PROSITE; PS01065; ETF_BETA; 1.
PE   1: Evidence at protein level;
KW   Electron transport; Reference proteome; Transport.
FT   CHAIN           1..256
FT                   /note="Protein FixA"
FT                   /id="PRO_0000167892"
FT   CONFLICT        216..217
FT                   /note="EA -> VD (in Ref. 1; CAA50797)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   256 AA;  27144 MW;  1954E0469748D310 CRC64;
     MKIITCYKCV PDEQDIAVNN ADGSLDFSKA DAKISQYDLN AIEAACQLKQ QAAEAQVTAL
     SVGGKALTNA KGRKDVLSRG PDELIVVIDD QFEQALPQQT ASALAAAAQK AGFDLILCGD
     GSSDLYAQQV GLLVGEILNI PAVNGVSKII SLTADTLTVE RELEDETETL SIPLPAVVAV
     STDINSPQIP SMKAILGAAK KPVQVWSAAD IGFNAEAAWS EQQVAAPKQR ERQRIVIEGD
     GEEQIAAFAE NLRKVI