ID   FIXB_AZOC5              Reviewed;         369 AA.
AC   P26483; A8IIT7;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Protein FixB;
GN   Name=fixB; OrderedLocusNames=AZC_3448;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1850088; DOI=10.1007/bf00261695;
RA   Arigoni F., Kaminski P.A., Hennecke H., Elmerich C.;
RT   "Nucleotide sequence of the fixABC region of Azorhizobium caulinodans
RT   ORS571: similarity of the fixB product with eukaryotic flavoproteins,
RT   characterization of fixX, and identification of nifW.";
RL   Mol. Gen. Genet. 225:514-520(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC   13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC       fixation.
CC   -!- SUBUNIT: FixA and FixB form a heterodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC       {ECO:0000305}.
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DR   EMBL; X55450; CAA39092.1; -; Genomic_DNA.
DR   EMBL; AP009384; BAF89446.1; -; Genomic_DNA.
DR   PIR; S14071; S14071.
DR   RefSeq; WP_012171971.1; NC_009937.1.
DR   AlphaFoldDB; P26483; -.
DR   SMR; P26483; -.
DR   STRING; 438753.AZC_3448; -.
DR   PRIDE; P26483; -.
DR   EnsemblBacteria; BAF89446; BAF89446; AZC_3448.
DR   KEGG; azc:AZC_3448; -.
DR   eggNOG; COG2025; Bacteria.
DR   HOGENOM; CLU_034178_1_1_5; -.
DR   OMA; YKHVWVF; -.
DR   OrthoDB; 1400253at2; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd01715; ETF_alpha; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR001308; ETF_a/FixB.
DR   InterPro; IPR033947; ETF_alpha_N.
DR   InterPro; IPR014731; ETF_asu_C.
DR   InterPro; IPR018206; ETF_asu_C_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR43153; PTHR43153; 1.
DR   Pfam; PF01012; ETF; 1.
DR   Pfam; PF00766; ETF_alpha; 1.
DR   PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR   SMART; SM00893; ETF; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   PROSITE; PS00696; ETF_ALPHA; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Nitrogen fixation;
KW   Reference proteome; Transport.
FT   CHAIN           1..369
FT                   /note="Protein FixB"
FT                   /id="PRO_0000167855"
FT   BINDING         299..327
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        14
FT                   /note="S -> T (in Ref. 1; CAA39092)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        261..262
FT                   /note="QL -> HV (in Ref. 1; CAA39092)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   369 AA;  39703 MW;  F4CD1913E228F190 CRC64;
     MSEPTKAPAA SAGSRAATKK ELPEHFKAYK HVWVFIEQER GQVHPVSWEL MGAGRKLADK
     LKVELAAIVL GPDDEATQHA AAEAFSYGAD LTYVVADPLL TDYRNEAYTK ALTDVVNTYK
     PEILLLGATT LGRDLAGSVA TTLLTGLTAD CTELDVDADG SLAATRPTFG GSLLCTIYTL
     NYRPQMATVR PRVMPMPERE EKPIGRIITH PLGMVEDDIV TKILSFLPDR DSAKSNLAYA
     DVVVAGGLGL GSPENFQLVR QLAGVLGAEY GCSRPLVQKG WVTSDRQIGQ TGKTIRPKLY
     IAAGISGAIQ HRVGVEGADM IVAINTDKNA PIFDFAHVGI VTDAIRLLPA LTEAFRARLS
     PHSRDRIAS