ID   FIXB_BRADU              Reviewed;         369 AA.
AC   P10449;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Protein FixB;
GN   Name=fixB; OrderedLocusNames=blr1773;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 3I1b110;
RX   PubMed=8599534; DOI=10.1007/bf01692858;
RA   Weidenhaupt M., Rossi P., Beck C., Fischer H.-M., Hennecke H.;
RT   "Bradyrhizobium japonicum possesses two discrete sets of electron transfer
RT   flavoprotein genes: fixA, fixB and etfS, etfL.";
RL   Arch. Microbiol. 165:169-178(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=11157954; DOI=10.1128/jb.183.4.1405-1412.2001;
RA   Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R.,
RA   Hennecke H.;
RT   "Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA
RT   region of the Bradyrhizobium japonicum chromosome.";
RL   J. Bacteriol. 183:1405-1412(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
RC   STRAIN=USDA 110spc4;
RX   PubMed=2503674; DOI=10.1111/j.1365-2958.1989.tb01803.x;
RA   Gubler M., Zurcher T., Hennecke H.;
RT   "The Bradyrhizobium japonicum fixBCX operon: identification of fixX and of
RT   a 5' mRNA region affecting the level of the fixBCX transcript.";
RL   Mol. Microbiol. 3:141-148(1989).
CC   -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC       fixation.
CC   -!- SUBUNIT: FixA and FixB form a heterodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC       {ECO:0000305}.
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DR   EMBL; U32227; AAB00903.1; -; Genomic_DNA.
DR   EMBL; AF322012; AAG60757.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47038.1; -; Genomic_DNA.
DR   EMBL; X13143; CAA31538.1; -; Genomic_DNA.
DR   PIR; S04183; S04183.
DR   RefSeq; NP_768413.1; NC_004463.1.
DR   RefSeq; WP_011084582.1; NZ_CP011360.1.
DR   AlphaFoldDB; P10449; -.
DR   SMR; P10449; -.
DR   STRING; 224911.27350026; -.
DR   EnsemblBacteria; BAC47038; BAC47038; BAC47038.
DR   GeneID; 64021612; -.
DR   KEGG; bja:blr1773; -.
DR   PATRIC; fig|224911.44.peg.1241; -.
DR   eggNOG; COG2025; Bacteria.
DR   HOGENOM; CLU_034178_1_1_5; -.
DR   InParanoid; P10449; -.
DR   OMA; YKHVWVF; -.
DR   PhylomeDB; P10449; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IBA:GO_Central.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd01715; ETF_alpha; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR001308; ETF_a/FixB.
DR   InterPro; IPR033947; ETF_alpha_N.
DR   InterPro; IPR014731; ETF_asu_C.
DR   InterPro; IPR018206; ETF_asu_C_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR43153; PTHR43153; 1.
DR   Pfam; PF01012; ETF; 1.
DR   Pfam; PF00766; ETF_alpha; 1.
DR   PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR   SMART; SM00893; ETF; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   PROSITE; PS00696; ETF_ALPHA; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Nitrogen fixation;
KW   Reference proteome; Transport.
FT   CHAIN           1..369
FT                   /note="Protein FixB"
FT                   /id="PRO_0000167857"
FT   BINDING         299..327
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   369 AA;  39527 MW;  F4C273384769553B CRC64;
     MSKVSKTAMP AGAGRGAAKK ELPEHFKSYK HVWVFVELER GQVHPVSWEL MGTGRGLADR
     LKVNLAAVVV GPEGQHTRNA ALEAFCYGAD LAYLVSDNVL SDYRNESYTK ALTELVNTYK
     PEILLLGATT LGRDLAGSVA TNLSTGLTAD CTTLDVDADG SLAATRPTFG GSLLCTIYTL
     NYRPQMATVR PRVMPMPARV MRDAARIVVH PLGLVEDDIV TKVLSFLPDR DAETSTLAYA
     DVVVAGGLGL GSPENFRLVR ELAALLGAEY GCSRPLVQKG WVTSDRQIGQ TGKTIRPKLY
     IAAGISGAIQ HRVGVEGADL IVAVNTDKNA PIFDFAHLAI VSDAMQLLPA LTAAFRARLL
     PDSRARIAV