ID   FIXB_RHIME              Reviewed;         353 AA.
AC   P09819;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Protein FixB;
GN   Name=fixB; OrderedLocusNames=RA0446; ORFNames=SMa0819;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=3029021; DOI=10.1128/jb.169.3.1127-1136.1987;
RA   Earl C.D., Ronson C.W., Ausubel F.M.;
RT   "Genetic and structural analysis of the Rhizobium meliloti fixA, fixB,
RT   fixC, and fixX genes.";
RL   J. Bacteriol. 169:1127-1136(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC       fixation.
CC   -!- SUBUNIT: FixA and FixB form a heterodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC       {ECO:0000305}.
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DR   EMBL; M15546; AAA21769.1; -; Genomic_DNA.
DR   EMBL; AE006469; AAK65104.1; -; Genomic_DNA.
DR   PIR; B26952; B26952.
DR   PIR; F95317; F95317.
DR   RefSeq; NP_435692.1; NC_003037.1.
DR   AlphaFoldDB; P09819; -.
DR   SMR; P09819; -.
DR   EnsemblBacteria; AAK65104; AAK65104; SMa0819.
DR   KEGG; sme:SMa0819; -.
DR   PATRIC; fig|266834.11.peg.459; -.
DR   HOGENOM; CLU_034178_1_1_5; -.
DR   OMA; YKHVWVF; -.
DR   Proteomes; UP000001976; Plasmid pSymA.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd01715; ETF_alpha; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR001308; ETF_a/FixB.
DR   InterPro; IPR033947; ETF_alpha_N.
DR   InterPro; IPR014731; ETF_asu_C.
DR   InterPro; IPR018206; ETF_asu_C_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR43153; PTHR43153; 1.
DR   Pfam; PF01012; ETF; 1.
DR   Pfam; PF00766; ETF_alpha; 1.
DR   PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR   SMART; SM00893; ETF; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   PROSITE; PS00696; ETF_ALPHA; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Nitrogen fixation; Plasmid;
KW   Reference proteome; Transport.
FT   CHAIN           1..353
FT                   /note="Protein FixB"
FT                   /id="PRO_0000167858"
FT   BINDING         283..311
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   353 AA;  37787 MW;  8142B04B4BC08546 CRC64;
     MKKGLPKQFQ DYRNVWVFIE LEHGQVHPVS IELLGEGRKL ADKLGVHLAG VVIGPPGGQG
     TANAIADAFA YGADLSYLVE SPLLAHYRNE PFTKALTDLV LANKPEILLL GATTLGRDLA
     GSVATTLKTG LTADCTELNV DSDGSLAATR PTFGGSLLCT IYTLKCRPQM ATVRPSVMAT
     PQRVNRPTGS IIRHDLKMLE EEIATKVLAF FSDCDSTIAN LAYADVVVAG GLGLGAVQNL
     QLLKDLARTL GGDFGCSRPL VQKGWMPFDR QIGQTGNTIR PKLYIAAGIS GAVQHRVGVE
     GSDLIVAINT DPNAPIFDFA HLGVVADAIS FLPALTEVFT KRLEPRNLEK FVQ