AKIR2_PIG
ID AKIR2_PIG Reviewed; 203 AA.
AC A0A287BDC1; F1S0E7; K7NCI8;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=Akirin-2 {ECO:0000305};
GN Name=AKIRIN2 {ECO:0000312|VGNC:VGNC:103023};
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=22537061; DOI=10.1080/10495398.2011.652326;
RA Chen X., Huang Z., Jia G., Wu X., Wu C.;
RT "Molecular cloning, tissue distribution, and functional analysis of porcine
RT Akirin2.";
RL Anim. Biotechnol. 23:124-131(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Guo Y.-J., Liu G.-Z., Wang C.-M., Wang Y.-Y., Zhu H.-S., Wang L.-F.,
RA Zhong K., Lu W.-F., Han L.-Q., Yang G.-Q., Li H.-J., Wang Y.-L.,
RA Yang G.-Y.;
RT "Cloning and prokaryotic expression of Sus scrofa Akirin2 gene and affinity
RT purification of its fused Protein.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=25686036; DOI=10.3390/ijms16023996;
RA Chen X., Luo Y., Zhou B., Huang Z., Jia G., Liu G., Zhao H., Yang Z.,
RA Zhang R.;
RT "Effect of porcine Akirin2 on skeletal myosin heavy chain isoform
RT expression.";
RL Int. J. Mol. Sci. 16:3996-4006(2015).
RN [5]
RP FUNCTION.
RX PubMed=28327665; DOI=10.1038/srep45156;
RA Chen X., Luo Y., Huang Z., Jia G., Liu G., Zhao H.;
RT "Akirin2 regulates proliferation and differentiation of porcine skeletal
RT muscle satellite cells via ERK1/2 and NFATc1 signaling pathways.";
RL Sci. Rep. 7:45156-45156(2017).
CC -!- FUNCTION: Molecular adapter that acts as a bridge between a variety of
CC multiprotein complexes, and which is involved in embryonic development,
CC immunity, myogenesis and brain development (By similarity). Plays a key
CC role in nuclear protein degradation by promoting import of proteasomes
CC into the nucleus: directly binds to fully assembled 20S proteasomes at
CC one end and to nuclear import receptor IPO9 at the other end, bridging
CC them together and mediating the import of pre-assembled proteasome
CC complexes through the nuclear pore (By similarity). Involved in innate
CC immunity by regulating the production of interleukin-6 (IL6) downstream
CC of Toll-like receptor (TLR): acts by bridging the NF-kappa-B inhibitor
CC NFKBIZ and the SWI/SNF complex, leading to promote induction of IL6 (By
CC similarity). Also involved in adaptive immunity by promoting B-cell
CC activation (By similarity). Involved in brain development: required for
CC the survival and proliferation of cerebral cortical progenitor cells
CC (By similarity). Involved in myogenesis: required for skeletal muscle
CC formation and skeletal development, possibly by regulating expression
CC of muscle differentiation factors (PubMed:25686036, PubMed:28327665).
CC {ECO:0000250|UniProtKB:B1AXD8, ECO:0000250|UniProtKB:Q53H80,
CC ECO:0000269|PubMed:25686036, ECO:0000269|PubMed:28327665}.
CC -!- SUBUNIT: Homodimer. Interacts with IPO9; the interaction is direct.
CC Associates with 20S and 26S proteasomes (By similarity). Interacts with
CC SMARCD1; promoting SWI/SNF complex recruitment. Interacts with NFKBIZ
CC (By similarity). Interacts with YWHAB (By similarity).
CC {ECO:0000250|UniProtKB:B1AXD8, ECO:0000250|UniProtKB:Q25C79,
CC ECO:0000250|UniProtKB:Q53H80}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B1AXD8}. Cytoplasm
CC {ECO:0000250|UniProtKB:B1AXD8}. Membrane
CC {ECO:0000250|UniProtKB:B1AXD8}. Note=Present mainly in the nuclear
CC fraction, and at much lower level in the cytoplasmic and membrane
CC fractions. {ECO:0000250|UniProtKB:B1AXD8}.
CC -!- TISSUE SPECIFICITY: Widely expressed (PubMed:22537061). Most abundant
CC in the lung, followed by the skeletal muscle, heart, liver, fat,
CC thymus, lymph node, small intestine, kidney and spleen
CC (PubMed:22537061). In skeletal muscle, expressed at higher level in
CC fast extensor digitorum longus (EDL) and longissimus lumborum (LL)
CC muscles than in slow soleus (SOL) muscles (PubMed:25686036).
CC {ECO:0000269|PubMed:22537061, ECO:0000269|PubMed:25686036}.
CC -!- SIMILARITY: Belongs to the akirin family. {ECO:0000305}.
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DR EMBL; JN227885; AER35882.1; -; mRNA.
DR EMBL; KC140110; AGA94528.1; -; mRNA.
DR EMBL; AEMK02000001; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001265681.1; NM_001278752.1.
DR STRING; 9823.ENSSSCP00000004651; -.
DR PaxDb; A0A287BDC1; -.
DR Ensembl; ENSSSCT00000004762; ENSSSCP00000004651; ENSSSCG00000004307.
DR Ensembl; ENSSSCT00015050447; ENSSSCP00015020126; ENSSSCG00015037910.
DR Ensembl; ENSSSCT00025087601; ENSSSCP00025038183; ENSSSCG00025063838.
DR Ensembl; ENSSSCT00030092315; ENSSSCP00030042479; ENSSSCG00030066045.
DR Ensembl; ENSSSCT00035045193; ENSSSCP00035018077; ENSSSCG00035034100.
DR Ensembl; ENSSSCT00040101685; ENSSSCP00040045819; ENSSSCG00040073652.
DR Ensembl; ENSSSCT00045048408; ENSSSCP00045033655; ENSSSCG00045028345.
DR Ensembl; ENSSSCT00050094710; ENSSSCP00050040829; ENSSSCG00050069421.
DR Ensembl; ENSSSCT00055006100; ENSSSCP00055004785; ENSSSCG00055003144.
DR Ensembl; ENSSSCT00060070825; ENSSSCP00060030557; ENSSSCG00060052031.
DR Ensembl; ENSSSCT00065081977; ENSSSCP00065035704; ENSSSCG00065059848.
DR Ensembl; ENSSSCT00070049327; ENSSSCP00070041657; ENSSSCG00070024713.
DR GeneID; 100519991; -.
DR KEGG; ssc:100519991; -.
DR CTD; 55122; -.
DR VGNC; VGNC:103023; AKIRIN2.
DR eggNOG; KOG4330; Eukaryota.
DR GeneTree; ENSGT00940000156096; -.
DR HOGENOM; CLU_119227_0_0_1; -.
DR InParanoid; A0A287BDC1; -.
DR OMA; RRCAPIM; -.
DR OrthoDB; 1420469at2759; -.
DR TreeFam; TF317123; -.
DR Proteomes; UP000008227; Chromosome 1.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000004307; Expressed in oocyte and 45 other tissues.
DR ExpressionAtlas; A0A287BDC1; baseline and differential.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISS:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0021987; P:cerebral cortex development; ISS:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0071630; P:nuclear protein quality control by the ubiquitin-proteasome system; ISS:UniProtKB.
DR GO; GO:0002821; P:positive regulation of adaptive immune response; ISS:UniProtKB.
DR GO; GO:0045089; P:positive regulation of innate immune response; ISS:UniProtKB.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0031144; P:proteasome localization; ISS:UniProtKB.
DR GO; GO:0006606; P:protein import into nucleus; ISS:UniProtKB.
DR GO; GO:0051147; P:regulation of muscle cell differentiation; IMP:UniProtKB.
DR InterPro; IPR024132; Akirin.
DR PANTHER; PTHR13293; PTHR13293; 1.
PE 2: Evidence at transcript level;
KW Adaptive immunity; Cytoplasm; Developmental protein; Immunity;
KW Innate immunity; Membrane; Nucleus; Phosphoprotein; Protein transport;
KW Reference proteome; Repressor; Transcription; Transcription regulation;
KW Transport.
FT CHAIN 1..203
FT /note="Akirin-2"
FT /id="PRO_0000455440"
FT MOTIF 22..27
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:Q53H80"
FT MOTIF 200..203
FT /note="SYVS motif"
FT /evidence="ECO:0000250|UniProtKB:Q53H80"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q53H80"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q53H80"
FT MOD_RES 57
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:B1AXD8"
FT CONFLICT 18
FT /note="S -> N (in Ref. 1; AER35882)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 203 AA; 22510 MW; 7B832871D06677E2 CRC64;
MACGATLKRT LDFDPLLSPA SPKRRRCAPL SAPTSAAASP SSAAAATAAS FSAAAASPQK
YLRMEPSPFG DVSSRLTTEQ ILYNIKQEYK RMQKRRHLET SFQQTDPCCT SDAQPHAFLL
SGPASPGTPS ATSSPLKKEQ PLFTLRQVGM ICERLLKERE EKVREEYEEI LNTKLAEQYD
AFVKFTHDQI MRRYGEQPAS YVS
