ID   FIXB_SINFN              Reviewed;         369 AA.
AC   Q53209;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Protein FixB;
GN   Name=fixB; OrderedLocusNames=NGR_a01230; ORFNames=y4vA;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8796346; DOI=10.1101/gr.6.7.590;
RA   Freiberg C., Perret X., Broughton W.J., Rosenthal A.;
RT   "Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234
RT   using dye terminators and a thermostable 'sequenase': a beginning.";
RL   Genome Res. 6:590-600(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC       fixation.
CC   -!- SUBUNIT: FixA and FixB form a heterodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC       {ECO:0000305}.
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DR   EMBL; Z68203; CAA92416.1; -; Genomic_DNA.
DR   EMBL; U00090; AAB91889.1; -; Genomic_DNA.
DR   RefSeq; NP_444102.1; NC_000914.2.
DR   RefSeq; WP_010875161.1; NC_000914.2.
DR   AlphaFoldDB; Q53209; -.
DR   SMR; Q53209; -.
DR   STRING; 394.NGR_a01230; -.
DR   EnsemblBacteria; AAB91889; AAB91889; NGR_a01230.
DR   KEGG; rhi:NGR_a01230; -.
DR   PATRIC; fig|394.7.peg.107; -.
DR   eggNOG; COG2025; Bacteria.
DR   HOGENOM; CLU_034178_1_1_5; -.
DR   OMA; LCKKHRP; -.
DR   OrthoDB; 1400253at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd01715; ETF_alpha; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR001308; ETF_a/FixB.
DR   InterPro; IPR033947; ETF_alpha_N.
DR   InterPro; IPR014731; ETF_asu_C.
DR   InterPro; IPR018206; ETF_asu_C_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR43153; PTHR43153; 1.
DR   Pfam; PF01012; ETF; 1.
DR   Pfam; PF00766; ETF_alpha; 1.
DR   PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR   SMART; SM00893; ETF; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   PROSITE; PS00696; ETF_ALPHA; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Nitrogen fixation; Plasmid;
KW   Reference proteome; Transport.
FT   CHAIN           1..369
FT                   /note="Protein FixB"
FT                   /id="PRO_0000167859"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         299..327
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   369 AA;  39851 MW;  C4B55DB56FA1C08A CRC64;
     MGTRKSKASP PAQARASMKK DLPEQFRDYR HVWVFMELER GHVHPVSFEL LGEGRRLADQ
     LGVQLAGIIL GPPGDSTLHA AAEAFAYGAD LVYLVEEPLL TDYRNEPFTK ALTDLVITHK
     PEILLLGATT LGRDLAGSVA TTLLTGLTAD CTGLDVDEDG SLAATRPTFG GSLLCTIYTL
     NCRPQMATVR PRVMRMPQRS NKPIGRVIRH DWRMLEEDIV TKVVGFLSDD RSGNANLAYA
     DVVVAGGLGL SNAENLLLIK DLALTIGGDY GCSRPLVQKG WMPADRQIGQ TGKTIRPKLY
     IAAGISGAVQ HRVGVEGADL IVAINTDPHA PIFDFAHVGV VTDAVSFLPA LTEAFTKRLA
     PANRLRVVN