ID   FIXC_ECOLI              Reviewed;         428 AA.
AC   P68644; P31575; P75626;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Protein FixC;
GN   Name=fixC; Synonyms=yaaS; OrderedLocusNames=b0043, JW0042;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA   Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA   Mizobuchi K., Nakata A.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT   2.4 min region.";
RL   Nucleic Acids Res. 20:3305-3308(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 64;
RP   280 AND 310.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-188.
RC   STRAIN=O44:K74;
RX   PubMed=7473063; DOI=10.1002/jobm.3620350404;
RA   Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A.;
RT   "The fix Escherichia coli region contains four genes related to carnitine
RT   metabolism.";
RL   J. Basic Microbiol. 35:217-227(1995).
RN   [5]
RP   PROBABLE FUNCTION.
RC   STRAIN=K12 / BW25113;
RX   PubMed=12081978; DOI=10.1128/jb.184.14.4044-4047.2002;
RA   Walt A., Kahn M.L.;
RT   "The fixA and fixB genes are necessary for anaerobic carnitine reduction in
RT   Escherichia coli.";
RL   J. Bacteriol. 184:4044-4047(2002).
CC   -!- FUNCTION: Could be part of an electron transfer system required for
CC       anaerobic carnitine reduction.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the ETF-QO/FixC family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC73154.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAB96611.2; -; Genomic_DNA.
DR   EMBL; X71977; CAA50799.1; -; Genomic_DNA.
DR   PIR; C64725; C64725.
DR   RefSeq; NP_414585.1; NC_000913.3.
DR   RefSeq; WP_001287715.1; NZ_STEB01000010.1.
DR   AlphaFoldDB; P68644; -.
DR   SMR; P68644; -.
DR   BioGRID; 4262204; 7.
DR   BioGRID; 853217; 1.
DR   IntAct; P68644; 8.
DR   STRING; 511145.b0043; -.
DR   PaxDb; P68644; -.
DR   PRIDE; P68644; -.
DR   EnsemblBacteria; AAC73154; AAC73154; b0043.
DR   EnsemblBacteria; BAB96611; BAB96611; BAB96611.
DR   GeneID; 66671667; -.
DR   GeneID; 948958; -.
DR   KEGG; ecj:JW0042; -.
DR   KEGG; eco:b0043; -.
DR   PATRIC; fig|1411691.4.peg.2240; -.
DR   EchoBASE; EB1525; -.
DR   eggNOG; COG0644; Bacteria.
DR   HOGENOM; CLU_050977_0_0_6; -.
DR   InParanoid; P68644; -.
DR   OMA; GEWAVRI; -.
DR   PhylomeDB; P68644; -.
DR   BioCyc; EcoCyc:EG11564-MON; -.
DR   PRO; PR:P68644; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009437; P:carnitine metabolic process; IEP:EcoliWiki.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR039651; FixC-like.
DR   PANTHER; PTHR43624; PTHR43624; 1.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Oxidoreductase; Reference proteome;
KW   Transport.
FT   CHAIN           1..428
FT                   /note="Protein FixC"
FT                   /id="PRO_0000200690"
FT   CONFLICT        1..3
FT                   /note="MSE -> MT (in Ref. 4)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        64
FT                   /note="D -> E (in Ref. 1; CAA50799)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   428 AA;  45703 MW;  4D089EE9A30C7FBC CRC64;
     MSEDIFDAII VGAGLAGSVA ALVLAREGAQ VLVIERGNSA GAKNVTGGRL YAHSLEHIIP
     GFADSAPVER LITHEKLAFM TEKSAMTMDY CNGDETSPSQ RSYSVLRSKF DAWLMEQAEE
     AGAQLITGIR VDNLVQRDGK VVGVEADGDV IEAKTVILAD GVNSILAEKL GMAKRVKPTD
     VAVGVKELIE LPKSVIEDRF QLQGNQGAAC LFAGSPTDGL MGGGFLYTNE NTLSLGLVCG
     LHHLHDAKKS VPQMLEDFKQ HPAVAPLIAG GKLVEYSAHV VPEAGINMLP ELVGDGVLIA
     GDAAGMCMNL GFTIRGMDLA IAAGEAAAKT VLSAMKSDDF SKQKLAEYRQ HLESGPLRDM
     RMYQKLPAFL DNPRMFSGYP ELAVGVARDL FTIDGSAPEL MRKKILRHGK KVGFINLIKD
     GMKGVTVL