FIXG_RHIME
ID FIXG_RHIME Reviewed; 524 AA.
AC P18396;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Nitrogen fixation protein FixG;
GN Name=fixG; OrderedLocusNames=RA0661; ORFNames=SMa1211;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymA (megaplasmid 1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=2536685; DOI=10.1128/jb.171.2.929-939.1989;
RA Kahn D., David M., Domergue O., Daveran M.-L., Ghai J., Hirsch P.R.,
RA Batut J.;
RT "Rhizobium meliloti fixGHI sequence predicts involvement of a specific
RT cation pump in symbiotic nitrogen fixation.";
RL J. Bacteriol. 171:929-939(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481432; DOI=10.1073/pnas.161294798;
RA Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA Long S.R.;
RT "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT meliloti pSymA megaplasmid.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: FixG is predicted to be involved in a redox process. The four
CC proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound
CC complex coupling the FixI cation pump with a redox process catalyzed by
CC FixG.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
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DR EMBL; Z21854; CAA79905.1; -; Genomic_DNA.
DR EMBL; AE006469; AAK65319.1; -; Genomic_DNA.
DR PIR; A32052; A32052.
DR PIR; E95344; E95344.
DR RefSeq; NP_435907.1; NC_003037.1.
DR RefSeq; WP_010967640.1; NC_003037.1.
DR AlphaFoldDB; P18396; -.
DR SMR; P18396; -.
DR EnsemblBacteria; AAK65319; AAK65319; SMa1211.
DR GeneID; 61599429; -.
DR KEGG; sme:SMa1211; -.
DR PATRIC; fig|266834.11.peg.681; -.
DR HOGENOM; CLU_032118_0_0_5; -.
DR OMA; PQEFFFV; -.
DR Proteomes; UP000001976; Plasmid pSymA.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1060.10; -; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR014116; Cyt_c_oxidase_cbb3_FixG.
DR InterPro; IPR032879; FixG_C.
DR InterPro; IPR009051; Helical_ferredxn.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF13746; Fer4_18; 1.
DR Pfam; PF12801; Fer4_5; 1.
DR Pfam; PF11614; FixG_C; 1.
DR TIGRFAMs; TIGR02745; ccoG_rdxA_fixG; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 1.
PE 4: Predicted;
KW 4Fe-4S; Cell membrane; Electron transport; Iron; Iron-sulfur; Membrane;
KW Metal-binding; Nitrogen fixation; Plasmid; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..524
FT /note="Nitrogen fixation protein FixG"
FT /id="PRO_0000159236"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 272..299
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 280
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 283
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 286
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 290
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 304
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 307
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 310
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 314
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 524 AA; 58304 MW; 1B0151AF063F28F0 CRC64;
MLHQPKTKAT VGRLDAETVN AARVRGPLYE KRRKIFPKRA EGRFRRFKWL VMLVTLGIYY
LTPWIRWDRG AHAPDQAVLI DLASRRFYFF FIEIWPQEFF FVAGLLVMAG FGLFLVTSAV
GRAWCGYACP QTVWVDLFLV VERFIEGDRN ARMRLDAGPW SLDKIRKRVA KHAIWVAIGV
ATGGAWIFYF ADAPSLMSSL VALDAPPVAY TTIGILTATT YVFGGLMREQ VCTYMCPWPR
IQAAMLDENS LVVTYNDWRG EPRSRHAKKA AAAGEVVGDC VDCNACVAVC PMGIDIRDGQ
QLECITCALC IDACDGVMDK LGRERGLISY ATLSDYAANM ALATSGGTAA IDPSRVRNAH
GAFRDKVRHL NWRIVFRPRV LVYFGVWATV GFGLLFGLLA RDRLELNVLH DRNPQFVVES
DGSVRNGYMV KLLNMIPEQR TISLTIEGMP AATMRVAGQA TGDGRRVTIG VEPDKVTPLK
VFVTLPKGRF AEAEEGFSLI AEDPSSHERD VYQANFNLPG AAGR
