ID   FIXJ_AZOC5              Reviewed;         211 AA.
AC   P26487; A8I019;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Transcriptional regulatory protein FixJ;
GN   Name=fixJ; OrderedLocusNames=AZC_4655;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2046550; DOI=10.1111/j.1365-2958.1991.tb00738.x;
RA   Kaminski P.A., Elmerich C.;
RT   "Involvement of fixLJ in the regulation of nitrogen fixation in
RT   Azorhizobium caulinodans.";
RL   Mol. Microbiol. 5:665-673(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC   13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: FixJ, when activated by FixL, induces the expression of both
CC       nifA, required for activation of classical nif and fix genes, and fixK,
CC       required for fixN activation.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by FixL. {ECO:0000305}.
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DR   EMBL; X56658; CAA39980.1; -; Genomic_DNA.
DR   EMBL; AP009384; BAF90653.1; -; Genomic_DNA.
DR   PIR; S15167; S15167.
DR   RefSeq; WP_012173174.1; NC_009937.1.
DR   AlphaFoldDB; P26487; -.
DR   SMR; P26487; -.
DR   STRING; 438753.AZC_4655; -.
DR   EnsemblBacteria; BAF90653; BAF90653; AZC_4655.
DR   KEGG; azc:AZC_4655; -.
DR   eggNOG; COG4566; Bacteria.
DR   HOGENOM; CLU_000445_90_4_5; -.
DR   OMA; KMNKHIA; -.
DR   OrthoDB; 930905at2; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Magnesium; Metal-binding;
KW   Nitrogen fixation; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..211
FT                   /note="Transcriptional regulatory protein FixJ"
FT                   /id="PRO_0000081100"
FT   DOMAIN          6..120
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          136..201
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        160..179
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   BINDING         11
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         57
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         55
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   211 AA;  22283 MW;  E0F06B3EA4CD827D CRC64;
     MPESLPVHVI DDDDAVRESL AFLLESSGLA VTQHTSAAAF LDAGVPLDRG CIVTDVRMPG
     ISGLELLKEL NARGAHMAVI VMTGHGDVPL AVEAMKLGAA DFLEKPFDDA AIIAAVRASL
     GRSAEQGRQE DARSEVGKRI AGLSQRERQV LECLVNGLAN KTIAYDLGIS PRTVEVYRAN
     VMTKMKAASL PELVRMALLA GVAPADDATP T